Biological effects of carbon ion beams with various LETs on budding yeast Saccharomyces cerevisiae

Matuo, Youichirou; Izumi, Yoshinobu; Furusawa, Yoshiya; Shimizu, Kikuo

doi:10.1016/j.mrfmmm.2017.10.003

Cited by 12 publications

(17 citation statements)

References 30 publications

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“…The sequence analysis of mutation in ura3 gene showed γ-ray/carbon ion induced transversions (66.7/68.7 %), transitions (20.0/13.7 %) and frameshifts (13.3/17.6 %) [8]. Transversions were mainly GC-TA and GC-CG and all of the transitions were GC-AT.…”

Section: Discussionmentioning

confidence: 99%

“…It seems that the sample size of ura3-mutants was not sufficient. It is very interesting that the spectrum of substitutions induced by heavy ions was sequence dependent and that there were hotspots [8]. Yeast mutation sites were localized near linker regions of nucleosomes, whereas mutations induced by γ-rays were located uniformly throughout the gene.…”

Section: Discussionmentioning

confidence: 99%

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Genetic Effects After Heavy Ion Irradiation of Haploid and Diploid Yeast Cells

Koltovaya¹,

Lyubimova²,

Zhuchkina³

2019

RadJ

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We have investigated the biological effects induced by different accelerated ions ( 4 He, 11 B, 12 C, 15 N, and 20 Ne) with different energies and linear energy transfers (LETs) and determined their relative biological effectiveness (RBE) for lethal damage and gene mutations. In particular, base pair substitution induction by ionizing radiation in haploid and diploid yeast Saccharomyces cerevisiae has been studied. We have detected the GC-AT transition in the haploid strain and the AT-TA transversion in the diploid strain. The RBE dependence on LET for lethal mutations is described by a curve with a local maximum at LET of about 100 keV/μm. It is shown that the mutation frequency increases with increasing the dose up to 1000 Gy for diploid cells irradiated by different ions. A decrease in RBE with increasing LET has been observed for diploid cells. However, for haploid cells irradiated at doses of up to 100 Gy, the curves seem to have a plateau. The RBE dependence on LET for haploid cells is different and also has a plateau. But for substitution induction in haploid cells, an ion beam with a high LET (177 keV/μm) is less mutagenic than the one with a low LET (44-127 keV/μm). Therefore, we have obtained different biological effects of accelerated ions for haploid and diploid cells.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genetic Effects After Heavy Ion Irradiation of Haploid and Diploid Yeast Cells

Koltovaya¹,

Lyubimova²,

Zhuchkina³

2019

RadJ

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“…(3) The LET used in the present study was 40 Kev/μ, which produced several promising mutants. In previous studies, heavy‐ion beams with different LET have been found to exert varied biological effects on plants and microbial strains . Hence, the effects of heavy‐ion beams with different LETs on S. fungicidicus must be further examined, because the LET value might affect double‐strand breaks induction efficiency in irradiated cells .…”

Section: Discussionmentioning

confidence: 99%

Heavy‐ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus

Liu

et al. 2018

Engineering in Life Sciences

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To improve fermentative production of enduracidin, heavy‐ion beams generated by the Heavy Ion Research Facility in Lanzhou (HIRFL), China, were employed for the first time to generate mutations in Streptomyces fungicidicus. Initial screening detected 44 positive mutants with larger inhibition zone, which were subsequently tested based on flask fermentation. Finally, 20 mutants showed 20% increase in enduracidin production, when compared with the original strain. Among them, enduracidin production by the three mutants (M13, M30, and M34) was significantly higher than that by the original strain. In particular, mutant M30 exhibited highest enduracidin production, which was 114% higher than that obtained with the original strain. Following culture optimization, the maximal enduracidin yield obtained by M30 reached 918.5 mg/L in 10 days, which was 34% higher than that noted in the control.

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“…The rad52 strain was hypersensitive to the high-LET carbon ion beam (107 keV/µm) ( Figure 1). In our previous study, the rad52 strain also showed hypersensitivity to a carbon ion beam with a relatively low LET (13 keV/µm) [20]. It has been shown that DSBs are mainly repaired by HR or the non-homologous end joining (NHEJ) pathway.…”

Section: Survival Ratementioning

confidence: 97%

“…The URA3 gene region of the mutants was amplified by PCR using Ex Taq polymerase (Takara Bio, Otsu, Japan). The PCR protocol was as described previously [20]. The nucleotide sequences were determined using an ABI2300 system (Applied Biosystems, Foster City, CA, USA).…”

Section: Sequence Analysismentioning

confidence: 99%

Molecular Analysis of Carbon Ion-Induced Mutations in DNA Repair-Deficient Strains of Saccharomyces cerevisiae

Matuo

Izumi

Sakamoto

et al. 2019

QuBS

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Mutations caused by ion beams have been well-studied in plants, including ornamental flowers, rice, and algae. It has been shown that ion beams have several significantly interesting features, such as a high biological effect and unique mutation spectrum, which is in contrast to low linear energy transfer (LET) radiation such as gamma rays. In this study, the effects of double strand breaks and 8-oxo-2 -deoxyguanosine (8-oxodG) caused by ion-beam irradiation were examined. We irradiated repair-gene-inactive strains rad52, ogg1, and msh2 using carbon ion beams, analyzed the lethality and mutagenicity, and characterized the mutations. High-LET carbon ion-beam radiation was found to cause oxidative base damage, such as 8-oxodG, which can lead to mutations. The present observations suggested that nucleotide incorporation of oxidative damage gave only a limited effect on cell viability and genome fidelity. The ion-beam mutations occurred predominantly in 5 -ACA-3 sequences, and we detected a hotspot at around +79 to +98 in URA3 in wild-type and mutant lines, suggesting the presence of a mutation-susceptible region.

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Biological effects of carbon ion beams with various LETs on budding yeast Saccharomyces cerevisiae

Cited by 12 publications

References 30 publications

Genetic Effects After Heavy Ion Irradiation of Haploid and Diploid Yeast Cells

Genetic Effects After Heavy Ion Irradiation of Haploid and Diploid Yeast Cells

Heavy‐ion mutagenesis significantly enhances enduracidin production by Streptomyces fungicidicus

Molecular Analysis of Carbon Ion-Induced Mutations in DNA Repair-Deficient Strains of Saccharomyces cerevisiae

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