Biological Effects of a Root Conditioning Treatment on Periodontally Affected Teeth - An In Vitro Analysis

Abstract

The aim of this study was to evaluated the root surfaces modifications resulted by application of different chemicals agents, and their influence on the fibrin network and fibroblasts attachment. From 96 anterior mandibular human extracted incisor teeth, 192 dentin blocks of buccal and lingual surface were obtained and randomly divided into 6 groups: Cont-control group, which received no treatment; Root surface scaling and root planing (Srp); Citric acid-Srp; EDTA-Srp; Tetracycline capsule-Srp; Tetracycline gel-Srp. After dentin treatments the specimens were analyzed as follows: 1) demineralization level and residues of the product by scanning electron microscopy (SEM); 2) adhesion of blood components after 20 min of surface treatment by SEM; 3) fibroblast attachment after 24 h by SEM; 4) cell metabolism after 24 h by MTT assay. Data were analyzed using Fisher Exact, One-way ANOVA test followed by Dunn's test, Tukey test and Dunnett test (α=0.05). Citric acid, EDTA and Tetracycline gel resulted in adequate demineralization with no completely smear layer and smear plug removal on root dentin surface. Tetracycline capsule produced great tetracycline residues with several demineralization areas. Tetracycline gel and EDTA groups presented more fibroblast fixation than other experimental groups. The highest mean blood clot adhesion score was observed in roots treated with tetracycline gel. EDTA and Tetracycline gel surface treatment removed the smear layer over dentin surface and promoted adhesion of fibrin network and fibroblast cells attachment.

The difference of saline and sterile water for tetracycline hydrochloride solvents in cementum demineralization

“…The effect of demineralization with sterile water solvent for tetra HCl better than saline solvent, this means that sterile water as a solvent of tetra HCl is more effective for root cementum demineralization. This results was contrast with the findings of the Silva et al 23 study where used saline as the solvent of tetra HCl, who found the residues showed after application on the root surface are probably related to the components present into the capsules, which were not completely dissolved.…”

Establishment of Epithelial Attachment on Titanium Surface Coated with Platelet Activating Peptide

“…When epithelial attachment is lost due to periodontitis around teeth, gingival epithelium can be restored with functional BM-attachment to dental root surfaces when the root surface is scaled to expose fresh cementum, on which platelets can form activated aggregates [ 15 , 16 ]. Platelets provide the comprehensive machinery for a fibrin clot in epithelial healing with the proper apico-basal polarization.…”

The effect varied scanning electron microscopy desiccation techniques has on demineralized dentin

“…Nonetheless, air‐drying desiccation continues to be used in SEM studies of demineralized root surfaces (Bergenholtz & Babay, ; Gamal & Mailhot, ; Silva et al, ; Trombelli et al, ). A comparison of photomicrographs of these reports with other reports which utilized CO 2 desiccation specimen preparation often reveals variations and inconsistencies in expected results.…”