Biological effects in photodynamic treatment combined with electropermeabilization in wild and drug resistant breast cancer cells

Weżgowiec, Joanna; Kulbacka, Julita; Saczko, Jolanta; Rossowska, Joanna; Chodaczek, Grzegorz; Kotulska, Małgorzata

doi:10.1016/j.bioelechem.2018.04.008

Cited by 13 publications

(10 citation statements)

References 29 publications

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“…It was shown that MCF-7/DX cells were more susceptible to electroporation than wild-type breast cancer cells. Similar to our research, increased MDR1, GSTpi, and MRP7 protein levels after electroporation of breast cancer cells were reported [51]. The authors indicated that one of the factors that differ between normal and cancer cells is the fraction and localization of the negatively charged phospholipid PS [52].…”

Section: Discussionsupporting

confidence: 89%

The Impact of Extracellular Ca2+ and Nanosecond Electric Pulses on Sensitive and Drug-Resistant Human Breast and Colon Cancer Cells

Kulbacka

Rembiałkowska

Szewczyk

et al. 2021

Cancers

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(1) Background: Calcium electroporation (CaEP) is based on the application of electrical pulses to permeabilize cells (electroporation) and allow cytotoxic doses of calcium to enter the cell. (2) Methods: In this work, we have used doxorubicin-resistant (DX) and non-resistant models of human breast cancer (MCF-7/DX, MCF-7/WT) and colon cancer cells (LoVo, LoVo/DX), and investigated the susceptibility of the cells to extracellular Ca2+ and electric fields in the 20 ns–900 ns pulse duration range. (3) Results: We have observed that colon cancer cells were less susceptible to PEF than breast cancer cells. An extracellular Ca2+ (2 mM) with PEF was more disruptive for DX-resistant cells. The expression of glycoprotein P (MDR1, P-gp) as a drug resistance marker was detected by the immunofluorescent (CLSM) method and rhodamine-123 efflux as an MDR1 activity. MDR1 expression was not significantly modified by nanosecond electroporation in multidrug-resistant cells, but a combination with calcium ions significantly inhibited MDR1 activity and cell viability. (4) Conclusions: We believe that PEF with calcium ions can reduce drug resistance by inhibiting drug efflux activity. This phenomenon of MDR mechanism disruption seems promising in anticancer protocols.

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Section: Discussionsupporting

confidence: 89%

The Impact of Extracellular Ca2+ and Nanosecond Electric Pulses on Sensitive and Drug-Resistant Human Breast and Colon Cancer Cells

Kulbacka

Rembiałkowska

Szewczyk

et al. 2021

Cancers

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“…In our study the following electroporation parameters were applied: 8 square pulses, 100 µs width, delivered at 1 Hz frequency, which corresponded to ESOPE protocol [34]. Our previous study demonstrated that the application of electric pulses increases cell membrane permeability to propidium iodide in human breast adenocarcinoma cells [14,32,41,42]. The dose of DOX with no cytotoxic effect was determined using a metabolic activity assay.…”

Section: Discussionmentioning

confidence: 99%

Doxorubicin Assisted by Microsecond Electroporation Promotes Irreparable Morphological Alternations in Sensitive and Resistant Human Breast Adenocarcinoma Cells

et al. 2020

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Electroporation increases the transmembrane transport of molecules. The combination of electric pulses with cytostatic compounds is beneficial for cancer treatment. Doxorubicin (DOX) is a commonly used chemotherapeutic anticancer drug. Its fluorescence properties enable the investigation of drug distribution and metabolism. In this study, doxorubicin was enhanced by electroporation to eliminate cancer cells more effectively. The influence of electroporation on the drug uptake was evaluated in two cell lines: MCF-7/WT and MCF-7/DOX. The intracellular localization of doxorubicin and its impact on the intracellular structure organization were examined under a confocal microscope. Cellular effects were examined with the 3(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test that estimates the rate of metabolism in viable cells. The ultrastructure (TEM) of tumor cells subjected to the electric field was analyzed. An enhanced doxorubicin efficacy was observed in MCF-7/DOX cells after combination with electroporation. The response of the resistant cell line was revealed to be more sensitive to electric pulses. Electroporation-based methods may be attractive for cancer treatment in human breast adenocarcinoma, especially with acquired resistance. Electroporation enables a reduction of the effective dose of the drugs and the exposure time in this type of cancer, diminishing side effects of the systemic therapy.

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“…Among many compounds tested, that is, doxorubicin, 36 ruthenium (III) compound, 37 5-fluorouracil, 38 phthalocyanines, 39 photofrin, 40 curcumin, 41 the trans-platinum analogue trans-[PtCl 2 (3-hmpy) 2 ], 42 oxaliplatin, 43 betulinic acid, 44,45 oat b-glucan, 46 cyanine IR-775, 47 catechin, 48 and sodium decahydrodecaborate, 49 only calcium electroporation was extensively studied. [50][51][52][53][54][55][56][57][58] Calcium electroporation effectively causes tumor necrosis, leaving the normal tissue less affected, making it a very promising compound for further clinical evaluation as it does not belong to the antineoplastic drugs, thus precautions related to antineoplastic drugs regarding storage, handling, disposal, and toxicity and mutagenicity are not an issue for calcium electroporation.…”

Section: Recent Advances In Electrochemotherapy 205mentioning

confidence: 99%

Recent Advances in Electrochemotherapy

Čemažar

Serša

2019

Bioelectricity

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Electrochemotherapy is gaining recognition as an effective local therapy that uses systemically or intratumorally injected bleomycin or cisplatin with electroporation as a delivery system that brings drugs into the cells to exert their cytotoxic effects. Preclinical work is still ongoing, testing new drugs, seeking the best treatment combination with other treatment modalities, and exploring new sets of pulses for effective tissue electroporation. The applications of electrochemotherapy are being fully exploited in veterinary oncology, where electrochemotherapy, because of its simple execution, has a relatively good cost-benefit ratio and is used in the treatment of cutaneous tumors. In human oncology, electrochemotherapy is fully recognized as a local therapy for cutaneous tumors and metastases. Its effectiveness is being explored in combination with immunomodulatory drugs. However, the development of electrochemotherapy is directed into the treatment of deepseated tumors with a percutaneous approach. Because of the vast number of reports, this review discusses the articles published in the past 5 years.

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Biological effects in photodynamic treatment combined with electropermeabilization in wild and drug resistant breast cancer cells

Cited by 13 publications

References 29 publications

The Impact of Extracellular Ca2+ and Nanosecond Electric Pulses on Sensitive and Drug-Resistant Human Breast and Colon Cancer Cells

The Impact of Extracellular Ca2+ and Nanosecond Electric Pulses on Sensitive and Drug-Resistant Human Breast and Colon Cancer Cells

Doxorubicin Assisted by Microsecond Electroporation Promotes Irreparable Morphological Alternations in Sensitive and Resistant Human Breast Adenocarcinoma Cells

Recent Advances in Electrochemotherapy

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