Biological effect of varying peptide binding affinity 
to the <i>BoLA-DRB3*2703</i> allele

Alizadeh, Zahra; Karrow, Niel A.; Mallard, Bonnie A.

doi:10.1051/gse:2003016

Cited by 6 publications

(10 citation statements)

References 27 publications

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“…The present work confirms the existence of correlations between susceptibility to disease and a specific MHC haplotype reported in animal and man [1,5,10].…”

Section: Discussionsupporting

confidence: 91%

“…1). The CESFLQKN marker sequence which we observed could induce spatial modifications in the binding groove pockets leading to alterations in the binding efficiency of some peptides [1,10]. It is possible that these peptides are exposed to pathogen proteases and are either not recognized or poorly recognized by the T cell receptor.…”

Section: Discussionmentioning

confidence: 85%

“…PCR-RFLP genotyping of this BoLA class II haplotype of susceptibility classically involves three successive restriction digestions (RsaI, BstYI and HaeIII) as described by the official BoLA nomenclature reference group 1 . To improve the efficiency of such a genotyping, we developed a PCR amplification of specific alleles (PASA) to quickly and specifically identify this marker, using a forward PASA inner primer (5 -GCG GGC GGG GTT CCT GGA GAG ATC-34) located in the exon 2 region spanning the E (28) and S (30) marker amino acid positions (Fig.…”

Section: Marker Typingmentioning

confidence: 99%

“…The reaction is specific when three primers are incorporated: the two classical Bod 31 and Bod 32 outer primers 1 (Fig. 2). …”

Section: Marker Typingmentioning

confidence: 99%

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Selection assisted by a BoLA-DR/DQ haplotype against susceptibility to bovine dermatophilosis

et al. 2003

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-Bovine dermatophilosis is a severe skin infection of tropical ruminants inducing a severe loss in productivity and a 15% mortality rate. This disease is caused by the actinomycete bacterium Dermatophilus congolensis associated with the tick Amblyomma variegatum. Currently there are no prospects for a vaccine, and acaricide or antibiotic control is hampered by the development of chemoresistance. Animal breeders have observed that dermatophilosis susceptibility seems to be determined genetically, and we previously identified a BoLA-DRB3-DQB class II haplotype marker for high (R 2 = 0.96) susceptibility to the disease. With this marker, we developed a successful eugenic selection procedure for zebu Brahman cattle in Martinique (FWI). Over a period of five years, a marked reduction in disease prevalence, from 0.76 to 0.02 was achieved, and this low level has been maintained over the last two years. The selection procedure, based on a genetic marker system targeting the highly polymorphic BoLA locus, eliminates only those individuals which are at the highest risk of contracting the disease. In the present work, we discuss the properties of this system, including the "heterozygote advantage" and the "frequency dependence" theories, and examine their involvement in the biological mechanisms at the host/pathogen interface. We speculate on the exact role of the MHC molecules in the control of the disease, how the natural selection pressure imposed by the pathogens selectively maintains MHC diversity, and how our results can be practically applied for integrated control of dermatophilosis in developing countries.

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“…The present work confirms the existence of correlations between susceptibility to disease and a specific MHC haplotype reported in animal and man [1,5,10].…”

Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 85%

Section: Marker Typingmentioning

confidence: 99%

“…The reaction is specific when three primers are incorporated: the two classical Bod 31 and Bod 32 outer primers 1 (Fig. 2). …”

Section: Marker Typingmentioning

confidence: 99%

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Selection assisted by a BoLA-DR/DQ haplotype against susceptibility to bovine dermatophilosis

et al. 2003

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“…However, he explains, by quoting Alizadeh et al (23) that this cannot conclude that the information in the expression of CMH genes, does not improve the mechanisms involved in the resistance to diseases or that it is not one of the many tools that may be used to improve animal health and indirectly productivity in these particular populations.…”

Section: Resultsmentioning

confidence: 99%

Association of gene BoLA DRB3.2 with production traits in a dairy herd of Antioquia, Colombia

Lopéz-Herrera

2014

Rev MVZ Córdoba

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Objective. To determine the associations of BoLA DRB3.2 alleles present in Holstein and BON x Holstein cattle to production and milk quality traits in a dairy herd of Antioquia, Colombia. Materials and methods. Ninety-one cows, 66 Holstein and 25 BxH, were genotyped for the BoLA DRB3.2 gene, through PCR-RFLP technique. Furthermore, the association of the alleles of the gene BoLA DRB3.2 with milk yield (PL305), fat yield (PG305), protein yield (PP305) fat percentage (PGRA) and protein percentage (PPRO) were determined, using a general linear model. Results. Twenty-seven BoLA DRB3.2 alleles were identified; the most frequent alleles in Holstein were: BoLA DRB3.2*23, 22, and 24 with frequencies of: 0.159, 0.129, and 0.106, respectively and the most frequent alleles in BxH were: BoLA DRB3.2*23, 24 and 20 with frequencies of: 0.20, 0.140, and 0.120, respectively. Associations of BoLA DRB3.2 alleles with production and milk quality traits were also determined. In Holstein cows the BoLA DRB3.2*36 allele was associated with low PL305 (p≤0.01), high PGRA in multiparous cows (p≤0.05) and high PG305 in primiparous cows (p≤0.01). The BoLA DRB3.2*33 allele was associated with increased in the PPRO in multiparous cows (p≤0.01). In BXH cows only the BoLA DRB3*19 allele was associated with high PGRA (p≤0.05). Conclusions. The gene BoLA DRB3.2 shows high polymorphism in both groups; Holstein and BxH and some of its allelic variants were associated with production and milk quality traits.

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Breadth of the CD4+ T cell response to Anaplasma marginale VirB9-1, VirB9-2 and VirB10 and MHC class II DR and DQ restriction elements

et al. 2012

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MHC class II molecules influence antigen-specific CD4+ T-lymphocyte responses primed by immunization and infection. CD4+ T-cell responses are important for controlling infection by many bacterial pathogens including Anaplasma marginale, and are observed in cattle immunized with the protective A. marginale outer membrane (OM) vaccine. Immunogenic proteins that comprise the protective OM vaccine include type IV secretion system (T4SS) proteins VirB9-1, VirB9-2, and VirB10, candidates for inclusion in a multi-epitope vaccine. Our goal was to determine the breadth of the VirB9-1, VirB9-2, and VirB10 T-cell response and MHC class II restriction elements in six cattle with different MHC class II haplotypes, defined by DRB3, DQA, and DQB allele combinations for each animal. Overlapping peptides spanning each T4SS protein were tested in T-cell proliferation assays with autologous antigen presenting cells (APC) and artificial APC expressing combinations of bovine DR and DQ molecules. Twenty immunostimulatory peptides were identified; three representing two or more epitopes in VirB9-1, ten representing eight or more epitopes in VirB9-2, and seven representing seven or more epitopes in VirB10. Of eight DRA/DRB3 molecules, four presented 15 peptides, which was biased as DRA/DRB3*1201 presented ten and DRA/DRB3*1101 presented four peptides. Four DQA/DQB molecules composed of two intrahaplotype and two interhaplotype pairs presented seven peptides, of which five were uniquely presented by DQ molecules. In addition,three functional mixed isotype (DQA/DRB3) restriction elements were identified. The immunogenicity and broad MHC class II presentation of multiple VirB9-1, VirB9-2, and VirB10 peptide epitopes justify their testing as a multi-epitope vaccine against A. marginale.

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Biological effect of varying peptide binding affinity to the BoLA-DRB32703* allele

Cited by 6 publications

References 27 publications

Selection assisted by a BoLA-DR/DQ haplotype against susceptibility to bovine dermatophilosis

Selection assisted by a BoLA-DR/DQ haplotype against susceptibility to bovine dermatophilosis

Association of gene BoLA DRB3.2 with production traits in a dairy herd of Antioquia, Colombia

Breadth of the CD4+ T cell response to Anaplasma marginale VirB9-1, VirB9-2 and VirB10 and MHC class II DR and DQ restriction elements

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Biological effect of varying peptide binding affinity to the BoLA-DRB3*2703 allele

Cited by 6 publications

References 27 publications

Selection assisted by a BoLA-DR/DQ haplotype against susceptibility to bovine dermatophilosis

Selection assisted by a BoLA-DR/DQ haplotype against susceptibility to bovine dermatophilosis

Association of gene BoLA DRB3.2 with production traits in a dairy herd of Antioquia, Colombia

Breadth of the CD4+ T cell response to Anaplasma marginale VirB9-1, VirB9-2 and VirB10 and MHC class II DR and DQ restriction elements

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Biological effect of varying peptide binding affinity to the BoLA-DRB32703* allele