Biological Effect of Licochalcone C on the Regulation of PI3K/Akt/eNOS and NF-κB/iNOS/NO Signaling Pathways in H9c2 Cells in Response to LPS Stimulation

Abstract

Polyphenols compounds are a group molecules present in many plants. They have antioxidant properties and can also be helpful in the management of sepsis. Licochalcone C (LicoC), a constituent of Glycyrrhiza glabra, has various biological and pharmacological properties. In saying this, the effect of LicoC on the inflammatory response that characterizes septic myocardial dysfunction is poorly understood. The aim of this study was to determine whether LicoC exhibits anti-inflammatory properties on H9c2 cells that are stimulated with lipopolysaccharide. Our results have shown that LicoC treatment represses nuclear factor-κB (NF-κB) translocation and several downstream molecules, such as inducible nitric oxide synthase (iNOS), intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1). Moreover, LicoC has upregulated the phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt)/endothelial nitric oxide synthase (eNOS) signaling pathway. Finally, 2-(4-Morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one hydrochloride (LY294002), a specific PI3K inhibitor, blocked the protective effects of LicoC. These findings indicate that LicoC plays a pivotal role in cardiac dysfunction in sepsis-induced inflammation.

Licochalcone C induced apoptosis in human oral squamous cell carcinoma cells by regulation of the JAK2/STAT3 signaling pathway

“…31 Among these reported polyphenols, LCC has been reported to exhibit various pharmacological effects, such as the PTP1B enzyme inhibitory activity, 32 attenuation of inflammatory response, 7 cardio-protective effect, 7,33 and apoptosis, 10 but their mechanisms of action remain elusive. Furthermore, according to Franceschelli et al, 34 LCC did not show cytotoxic activity on cardiomyocyte cell line, H9c2 cells even at a concentration 250 μM. We investigated whether LCC could suppress cell growth and induce apoptosis through the regulation of the JAK2/ STAT3 pathway in OSCC cell lines (HN22 and HSC4).…”

Myricetin attenuated LPS induced cardiac injury in vivo and in vitro

“…The high expression level of iNOS from both macrophage and cardiomyocytes could lead to the systemic and cardiac dysfunction in sepsis (Franceschelli et al, ; Ndongson‐Dongmo et al, ). That might be partly related to increased production of superoxide and the resulting increased level of oxidative stress (Neri, Riezzo, Pomara, Schiavone, & Turillazzi, ).…”

Sodium butyrate ameliorates lipopolysaccharide‐induced cow mammary epithelial cells from oxidative stress damage and apoptosis

“…PI3K and its downstream effector, the serine/threonine kinase AKT/PKB, play a role in renal cellular survival or death . An LPS‐mediated‐AKT pathway was reported in different cell types, such as fibroblasts and neuroblastoma cells, which inhibits proapoptotic proteins, such as Bax . It was also demonstrated that NaB exerts antiapoptotic effects via the PI3K/AKT pathway on serum deprivation‐induced apoptosis in hepatic ischemia/reperfusion injured mice .…”