Biological detoxification and decolorization enhancement of azo dye by introducing natural electron mediators in MFCs

Li, Tao; Song, Hai-Liang; Xu, Han; Yang, Xiao-Li; Chen, Qiaoling

doi:10.1016/j.jhazmat.2021.125864

Cited by 35 publications

(7 citation statements)

References 63 publications

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“…30 The main possible reason was that some electrons of the anode surface were exploited to reduce the neocarmine dye molecules and break the azo chemical bonds. 31 The enrichment of electrons on the anode surface promoted the fracture of azo bonds, resulting in a high decolorization efficiency. The Fe/N-PCFF MFC obtained high decolorization efficiency, owing to the abundant electrons enriched in the anode surface by the thick and dense biofilm.…”

Section: Resultsmentioning

confidence: 99%

“…One of the reasons was that some parts of azo neocarmine dye molecules were reduced by the azo reductase of obligate or facultative anaerobic bacteria . The main possible reason was that some electrons of the anode surface were exploited to reduce the neocarmine dye molecules and break the azo chemical bonds . The enrichment of electrons on the anode surface promoted the fracture of azo bonds, resulting in a high decolorization efficiency.…”

Section: Resultsmentioning

confidence: 99%

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Fe/N Codoped Paper Carbon Fiber Foam Promoted Extracellular Electron Transfer for a High-Performance Microbial Fuel Cell

Jiang,

Cheng,

et al. 2023

ACS Appl. Eng. Mater.

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Anodes are considered an important component of microbial fuel cells (MFCs) to recover electric energy from organic wastewater. Herein, Fe/N codoped paper carbon fiber foam was developed as an anode for MFCs to enhance electricity generation and wastewater treatment. The interconnected microporous network was beneficial for bacteria colonization and biofilm formation. The N doping enhanced the hydrophilicity to facilitate bacterial proximity. The Fe doping modulation adhered to the microorganisms due to the positive charge of iron ions. The codoped anode promoted the organic matter degradation efficiency and intrinsic electricity generation property based on the optimized thick biofilm. The high electricity output of MFCs was increased by the decreased internal resistance. The assembled MFCs possessed a short start-up time of 29.1 h, high maximum power density of 2270 ± 118 mW·m–2, desirable chemical oxygen demand removal efficiency of 71.7 ± 1.9%, and high decolorization efficiency of 90.3 ± 2.5% at 44 h. Fe/N codoped strategy is proposed to construct anodes for improving the electricity generation property and wastewater treatment efficiency of MFCs.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Fe/N Codoped Paper Carbon Fiber Foam Promoted Extracellular Electron Transfer for a High-Performance Microbial Fuel Cell

Jiang,

Cheng,

et al. 2023

ACS Appl. Eng. Mater.

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“…Redox mediators involvement in bacterial -N=N-bond reductive cleavage under anaerobic condition have been reported (dos Santos et al, 2003; Van der Zee and Cervantes, 2009; Li et al, 2021), however, their preferences in microbial system and their participation in aerobic conditions for the dye decolorization remains vaguely defined. Flavin enzyme cofactors, such as flavin adenine dinucleotide (FAD), flavin adenine mononucleotide (FMN) and riboflavin, along with other quinone compounds, such as Anthraquinone-2, 6-disulfonate (AQDS), Anthraquinone-2-sulfonate (AQS), and lawsone, are known redox mediators.…”

Section: Resultsmentioning

confidence: 99%

Functional characterization of bacterial isolates from dye decolorizing consortia and a step-up metabolic engineering based on NADH-regeneration

Rathod

Archana

2022

Preprint

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Azo dye decolorizing acclimatized decolorizing consortia are enriched microbial sources of potential azoreductase-efficient bioremediation strains. Here, we characterized eight selected consortial members for their azo decolorization and azoreductase profiling. These efficient dye decolorizing bacterial isolates were affiliated to two major phyla viz. Firmicute (genus-Enterococcus) and Proteobacteria (γ-group). Redox-mediators such as AQDS and AQS were found to significantly increase decolorization except for menadione, and IR functional group signatures highlighted the azo bond reduction and degraded metabolites profiles of each strain. Among isolates, Enterococcus sp. L2 was found to be the most effective strain as it could reduce >90mg/L Reactive violet 5R (RV5R) dye in 3h of incubation. Furthermore, strain L2 possesses profound high NADH and NADPH-dependent azoreductase activity which also corroborated with its superior azo decolorization. As per physicochemical parameters, strain L2 showed an optimum decolorization at pH 8, 40 °C and up to 2% w/v salinity. To channelize reducing equivalence (NADH) to further enhance the dye decolorization in NADH-azoreductase efficient Enterococcus sp. L2, we augmented an NADH co-factor regeneration system. Using pMGS100, a Gram-positive expression vector a constitutive heterologous expression of Mycobacterium vaccae encoded NAD+-dependent formate dehydrogenase enhanced NADH pool which led to a significant 3.2 fold increased dye decolorization in Enterococcus sp. L2 harboring pMGS100 fdh along with a positive effect on growth. Ultimately, an augmentation of formate utilization step could further accelerate azo dye decolorization by fulfilling the co-factor (NADH) requirement of azoreductase along with a growth advantage in the non-model azoreductase-efficient environmentally important strain L2.

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“…In the work of Chen, H., et al (2021), a neutral red synthetic dye was used as mediator, achieving a 46% increase in its cell energy efficiency. The author Li, T., et al (2021), applied several natural azo dyes with a maximum energy efficiency of 5%, a coulombic efficiency of 6.45% and associated with the decolorization of toxic dyes, like this work, but with lower efficiency in all aspects.…”

Section: Energy Profile Of Electrochemical Mediation In a Bcmentioning

confidence: 99%

Microbial fuel cell (MFC) performance with pigments bikaverin and congo red as electrochemical mediators for optmization power energy

Silva

Nascimento

Campos-Takaki

2022

RSD

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For decades, non-renewable energy resources have been used indiscriminately, but their slow depletion and extremely harmful impacts on the environment have shifted the focus to sustainable and renewable energy sources. Among the renewable energy sources, biofuel cells are defined as devices that convert chemical energy present in chemical bonds into electrical energy. Biocells are classified into two broad categories of enzymatic fuel cells, which employ enzymes as biocatalysts, and microbial fuel cells, which use microorganisms as biocatalysts. An important requirement in the functioning of a biofuel cell is the transfer of electrons from inside an active site of an enzyme to the outside, as the electrodes being solid cannot penetrate the enzymes. A wide range of molecules can be used as electrochemical mediators, some with high toxicity and many non-toxic fungal substances having an enormous potential to be used as electrochemical mediators. In this work, the fungal pigment bikaverin was compared to the synthetic dye Congo red, in order to obtain the best energy-optimizing molecule in an enzymatic fuel cell. Congo red presented a higher current density of 273 mA.cm-2 compared to bikaverin, 230 mA.cm-2, but because it presents a more stable chronoamperometric graph and does not have high toxicity, the fungal biopigment proved to be the best option for optimization. on the potential of energy generated in an enzymatic fuel cell.

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Biological detoxification and decolorization enhancement of azo dye by introducing natural electron mediators in MFCs

Cited by 35 publications

References 63 publications

Fe/N Codoped Paper Carbon Fiber Foam Promoted Extracellular Electron Transfer for a High-Performance Microbial Fuel Cell

Fe/N Codoped Paper Carbon Fiber Foam Promoted Extracellular Electron Transfer for a High-Performance Microbial Fuel Cell

Functional characterization of bacterial isolates from dye decolorizing consortia and a step-up metabolic engineering based on NADH-regeneration

Microbial fuel cell (MFC) performance with pigments bikaverin and congo red as electrochemical mediators for optmization power energy

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