Biological control of sheep parasite nematodes by nematode-trapping fungi: in vitro activity and after passage through the gastrointestinal tract

Graminha, Érika Barbosa Neves; Costa, Alvimar José da; Oliveira, Gilson Pereira de; Monteiro, Ana Ruth Macêdo; Palmeira, Solange B. S.

doi:10.1007/s11274-004-4045-8

Cited by 13 publications

(12 citation statements)

References 23 publications

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“…Al igual que con A. oligospora, las características morfoló-gicas de A. musiformis observadas en el presente trabajo son coincidentes con lo encontrado en estudios llevados a cabo en otros países: crecimiento y extensión rápida de la colonia, conidios de 22-44 × 10-14 μm de longitud × 7,5-12,7 μm de ancho, en promedio (20)(21)(22)(23)(24)(25). Así mismo, se detectó que la formación de redes tridimensionales es la principal estructura de atrapamiento de estos dos hongos, resultados coincidentes con registros internacionales (12,22,(26)(27)(28)(29).…”

Section: Discussionunclassified

“…Así mismo, se detectó que la formación de redes tridimensionales es la principal estructura de atrapamiento de estos dos hongos, resultados coincidentes con registros internacionales (12,22,(26)(27)(28)(29).…”

Section: Discussionunclassified

“…Por tanto, la patogenicidad de las diferentes cepas de hongos nematófagos está determinada por la acción de enzimas hidrolíticas, proteasas, colagenasas, quitinasas y otras enzimas, las cuales, se ha demostrado, están influenciadas por la expresión genética que lleva consigo cada hongo (22,37). De esta forma, se determina que la acción y expresión de las diferentes enzimas producidas por los hongos nematófagos inciden en la inmovilización, penetración y degradación de las larvas; por lo tanto, es importante tener en cuenta la variabilidad genética entre individuos de una misma especie (24).…”

Section: Discussionunclassified

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Capacidad predadora in vitro de hongos nematófagos nativos de Cundinamarca sobre nematodos gastrointestinales de bovinos

Lara¹,

Burbano²,

Cárdenas³

et al. 2015

Rev. Med. Vet.

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La dependencia y el uso indiscriminado de antihelmínticos químicos como único método de control de los nematodos gastrointestinales (NGI) de bovinos ocasiona problemas en el medio ambiente, la salud pública y la productividad de la ganadería bovina. Es necesario desarrollar estrategias no químicas de control. Los hongos nematófagos pueden ser una alternativa viable y promisoria para el control de estos endoparásitos. El objetivo de este estudio fue aislar, identificar y evaluar in vitro el potencial nematófago de hongos de Cundinamarca sobre larvas L3 de NGI de bovinos. Se sembraron 60 muestras de suelo de fincas ganaderas en cajas de Petri con medio agar-agua para el atrapamiento de los hongos, y se identificaron mediante claves morfométricas tres cepas del hongo Arthrobotrys oligospora (L1, XVIII y XXI) y una de Arthrobotrys musiformis (XXIV). Se utilizaron 1 × 106 conidios o clamidosporas de cada hongo, los cuales se enfrentaron a 100 larvas de nematodos. El aislamiento XXIV (A. musiformis) demostró mayor capacidad predadora (96,8 %) que los aislamientos (A. oligospora) XVIII, L1 y XXI (69, 68, 71,1 y 87,62 %, respectivamente). No hubo diferencias estadísticamente significativas (p > 0,05) entre las cepas de mayor capacidad predadora. Este es el primer registro de identificación y evaluación in vitro de la capacidad predadora de los hongos A. oligospora y A. musiformis nativos de Cundinamarca. Los resultados sugieren que estos hongos podrían emplearse como agentes biocontroladores de nematodos de bovinos.

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Section: Discussionunclassified

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Capacidad predadora in vitro de hongos nematófagos nativos de Cundinamarca sobre nematodos gastrointestinales de bovinos

Lara¹,

Burbano²,

Cárdenas³

et al. 2015

Rev. Med. Vet.

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“…As the natural enemy of parasitic nematodes, the nematode-trapping fungi have emerged as a promising application with potential in biological control for nematode diseases in domestic animals (Mota et al 2003;Paraud and Chartier 2003;Terrill et al 2004;Graminha et al 2005;Dias et al 2007;Braga et al 2010). Under the induction of nematodes, the nematode-trapping fungi can form the structurally complex mycelia including constricting ring, adhesive net and adhesive knob.…”

Section: Introductionmentioning

confidence: 99%

Expression of serine proteinase P186 of Arthrobotrys oligospora and analysis of its nematode-degrading activity

Zhao

Qiao

Meng

et al. 2015

Antonie van Leeuwenhoek

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The nematode-trapping fungi possess a unique capability of predating and invading nematodes. As a representative nematode-trapping fungus, Arthrobotrys oligospora has been widely used to study the interactions between nematode-trapping fungi and their hosts. Serine proteinase is one of the important virulence factors during process of invasion of the nematode-trapping fungi into nematodes. In this study, using reverse transcription polymerase chain reaction, we amplified the gene sequence of serine proteinase 186 from A. oligospora, cloned it into pPIC9K vector and expressed it in the yeast Pichia pastoris. The expressed recombinant serine proteinase186 (reP186) was purified via Ni-affinity chromatography. The in vitro nematode-degrading activity of reP186 was analyzed. Sodium dodecyl sulfate polyacrylamide gel electrophoresis and Western blot analysis revealed that reP186 with molecular weight of 33 kDa was successfully obtained. ReP186 was capable of degrading a series of protein substrates including casein, gelatin, bovine serum albumin, denatured collagen and nematode cortical layer. The reP186 exhibited the maximal activity at pH 8.0 and 55 °C and was highly sensitive to the inhibitor, phenylmethanesulfonylfluoride. Treatment of Caenorhabditis elegans and Haemonchus contortus with reP186 for 12, 24 and 36 h, respectively, resulted in 62, 88 and 100 % of killing rates for C. elegans, and 52, 65 and 84 % of killing rates for H. contortus, respectively, indicating a relatively strong nematode-degrading bioactivity of reP186.

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“…Alternative measures for the control of helminths include improved nutrition (BRICARELLO et al, 2005;HOUDIJK et al, 2005), homeopathy (CAVALCANTI; ALMEIDA; ZACHARIAS et al, 2007), phytotherapy (CHANDRAWATHANI et al, 2006;CHAGAS et al, 2008), treatment with plant tannins (CENCI et al, 2007;MINHO et al, 2008MINHO et al, , 2010, supplementation with minerals (GONCALVES; ECHEVARRIA, 2004) and the use of nematophagous fungi (GRAMINHA et al, 2001(GRAMINHA et al, , 2005AMARANTE, 2007).…”

Section: Introductionmentioning

confidence: 99%

Supplementation with protected fat to manage gastro-intestinal nematode infections in Santa Ines sheep

Afonso

Costa

Filho³

et al. 2013

Sem. Ci. Agr.

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This experiment aimed to determine the influence of supplementation with protected fat on nematode infections in periparturient sheep or sheep in their final stage of pregnancy. Fifty Santa Ines ewes received 200 g of concentrate/animal/day, mineral salt and water and were grouped according to their weight, hematocrit and the number of eggs per gram of feces (EPG). Two diets were used: a control treatment and a treatment consisting of supplementation with 30 g/animal/day of protected fat in the concentrate. Three monthly evaluations were performed over a total of 84 days of testing. The variables analyzed were weight, body condition, EPG and coprocultures; blood tests were performed for the determination of packed cell volume, hemoglobin concentration and total plasma protein, and leukograms and eosinophil counts were performed. For weight and hemoglobin concentrations, interactions were observed between diet and collection date (P<0.05); body condition, total plasma protein, packed cell volume and total leukocytes did not differ statistically among treatments (P> 0. 05) but did differ by collection day (P <0.05). The EPG and eosinophil counts did not differ statistically by either diet or collection date (P>0.05). The genus Haemonchus was predominant, followed by the genera Cooperia, Trichostrongylus and Oesophagostomum. The protected fat did not decrease EPG and did not improve the blood parameters of infected sheep. Key words: Polyunsaturated fatty acids, nutrition, sheep, Haemonchus ResumoO objetivo do experimento foi verificar a influência da suplementação com gordura protegida na infecção por nematódeos gastrintestinais em ovelhas Santa Ines no periparto ou terço final de gestação. Cinquenta ovelhas Santa Inês receberam 200 g de concentrado/animal/dia, sal mineral e água, sendo divididas de acordo com peso, número de ovos por grama de fezes (OPG) e porcentagem do hematócrito em duas dietas, consideradas como tratamento controle e tratamento com suplementação de 30g/dia/animal de gordura protegida no concentrado. Duas avaliações mensais foram realizadas durante três meses, totalizando 84 dias de experimentação. As variáveis analisadas foram peso e condição corporal, OPG e coprocultura, contagem de leucócitos e eosinófilos, verificação do volume globular, concentração de hemoglobina e dosagem de proteína plasmática total. Em relação as variáveis peso e hemoglobina, houve interação entre as dietas e as coletas (P<0,05); as variáveis condição corporal, proteína plasmática total, volume globular e leucócitos totais não apresentaram diferença estatística entre as dietas (P>0,05) mas sim durante as coletas (P<0,05). As variáveis OPG e eosinófilos não apresentaram diferença estatística (P>0,05). O gênero Haemonchus foi predominante, seguido pelos gêneros Cooperia, Trichostrongylus and Oesophagostomum A suplementação com gordura protegida na quantidade de 30g/animal/dia, não interferiu na diminuição do OPG ou melhora dos parâmetros sanguíneos em infecção parasitária nas ovelhas deste estudo.

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Biological control of sheep parasite nematodes by nematode-trapping fungi: in vitro activity and after passage through the gastrointestinal tract

Cited by 13 publications

References 23 publications

Capacidad predadora in vitro de hongos nematófagos nativos de Cundinamarca sobre nematodos gastrointestinales de bovinos

Capacidad predadora in vitro de hongos nematófagos nativos de Cundinamarca sobre nematodos gastrointestinales de bovinos

Expression of serine proteinase P186 of Arthrobotrys oligospora and analysis of its nematode-degrading activity

Supplementation with protected fat to manage gastro-intestinal nematode infections in Santa Ines sheep

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