Biological Control of Root-Knot Nematodes by Organic Acid-Producing Lactobacillus brevis WiKim0069 Isolated from Kimchi

Seo, Hye Jeong; Park, Ae Ran; Kim, Seulbi; Yeon, Jehyeong; Yu, Nan Hee; Ha, Sanghyun; Chang, Ji Yoon; Park, Hae Woong; Kim, Jin-Cheol

doi:10.5423/ppj.oa.08.2019.0225

Cited by 28 publications

(18 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our results demonstrated that two nematicidal sesquiterpenoids ArA and DHArA exist in the methanol extract of A. annua L., which had not been reported before. Natural small molecule organic acids have been well demonstrated as nematicides to control nematodes effectively [15–18] or as a chemical inducer to induce plant resistance against root‐knot nematodes [19] . Particularly, it is reported that the organic acids acetic acid, butyric acid, and hexanoic acid displayed the potent nematicidal activity against M. incognita J2s with EC 50/24 h values of 38.3 μg/mL (0.64 mM), 40.7 μg/mL (0.46 mM) and 41.1 μg/mL (0.35 mM), respectively [18] .…”

Section: Discussionmentioning

confidence: 99%

Bioassay‐Guided Isolation of Nematicidal Artemisinic Acid and Dihydroartemisinic Acid from Artemisia annua L. and Evaluation of Their Activity against Meloidogyne incognita

Wang

Yuan

et al. 2022

Chemistry & Biodiversity

View full text Add to dashboard Cite

The extract of Artemisia annua L. has been shown to possess the nematicidal activity but the potent constituents were unclear. Herein, two nematicidal sesquiterpenoid acids artemisinic acid (ArA) and dihydroartemisinic acid (DHArA) were isolated from the methanol extract of the aerial parts of A. annua L. by the bioassay‐guided isolation method using Meloidogyne incognita second stage juveniles (J2s) as the screening target. The in vitro activity, control efficacy in the pot experiment, and toxic effects of these two natural compounds against M. incognita were evaluated. The in vitro results showed that the EC50/48h values of ArA and DHArA were 0.37 mM and 0.76 mM against J2s, respectively. In the pot experiment, ArA and DHArA at the dose of 5 mg (a.i.)/pot could achieve the same level of control efficacy compared with avermectins at 2 mg (a.i.)/pot. Microscopic observations indicated the obvious toxic symptoms of J2s after ArA‐ and DHArA‐treatment, including the shrinking body, imperfect body wall, and undiscerning organs. The physiological and biochemical studies, together with the toxic symptoms, revealed that ArA and DHArA had great impacts on the membrane system of J2s. Additionally, ArA occurring the α, β‐unsaturated carbonyl was demonstrated to be reactive with glutathione (GSH) and cause the decrease of GSH content in J2s. Taken together, the present study suggests that ArA and DHArA or ArA‐ and DHArA‐based extracts of A. annua L. have a substantial potential to be used as botanical agents for integrated disease management programs against root‐knot nematodes in crop protection.

show abstract

Section: Discussionmentioning

confidence: 99%

Bioassay‐Guided Isolation of Nematicidal Artemisinic Acid and Dihydroartemisinic Acid from Artemisia annua L. and Evaluation of Their Activity against Meloidogyne incognita

Wang

Yuan

et al. 2022

Chemistry & Biodiversity

View full text Add to dashboard Cite

show abstract

“…In contrast, not all of these groups are exclusively related to plants. For instance, acetic acid is produced as secondary plant metabolites [ 53 ] or as culture filtrates of the bacterium, Lactobacillus brevis , strain WiKim0069 [ 54 ]. This acid can damage the cuticle of RKN J 2 , vacuolize the cytoplasm, and degrade the nuclei, causing death [ 54 ].…”

Section: Botanicals As Bionematicidesmentioning

confidence: 99%

“…For instance, acetic acid is produced as secondary plant metabolites [ 53 ] or as culture filtrates of the bacterium, Lactobacillus brevis , strain WiKim0069 [ 54 ]. This acid can damage the cuticle of RKN J 2 , vacuolize the cytoplasm, and degrade the nuclei, causing death [ 54 ]. Numerous organic acids, such as amino, propionic, formic, and butyric acids, can exert toxic effects on PPN species [ 18 ].…”

Section: Botanicals As Bionematicidesmentioning

confidence: 99%

Optimizing Safe Approaches to Manage Plant-Parasitic Nematodes

Abd-Elgawad

2021

Plants

View full text Add to dashboard Cite

Plant-parasitic nematodes (PPNs) infect and cause substantial yield losses of many foods, feed, and fiber crops. Increasing concern over chemical nematicides has increased interest in safe alternative methods to minimize these losses. This review focuses on the use and potential of current methods such as biologicals, botanicals, non-host crops, and related rotations, as well as modern techniques against PPNs in sustainable agroecosystems. To evaluate their potential for control, this review offers overviews of their interactions with other biotic and abiotic factors from the standpoint of PPN management. The positive or negative roles of specific production practices are assessed in the context of integrated pest management. Examples are given to reinforce PPN control and increase crop yields via dual-purpose, sequential, and co-application of agricultural inputs. The involved PPN control mechanisms were reviewed with suggestions to optimize their gains. Using the biologicals would preferably be backed by agricultural conservation practices to face issues related to their reliability, inconsistency, and slow activity against PPNs. These practices may comprise offering supplementary resources, such as adequate organic matter, enhancing their habitat quality via specific soil amendments, and reducing or avoiding negative influences of pesticides. Soil microbiome and planted genotypes should be manipulated in specific nematode-suppressive soils to conserve native biologicals that serve to control PPNs. Culture-dependent techniques may be expanded to use promising microbial groups of the suppressive soils to recycle in their host populations. Other modern techniques for PPN control are discussed to maximize their efficient use.

show abstract

“…The control of RKN disease with organic acids has been studied for many years (Akhtar and Malik 2000;McBride et al 2000). Oxalic acid (OA), an organic acid, has shown nematicidal activity on several nematode species (Jang et al 2016;Seo et al 2019;Yeon et al 2019;Zuckerman et al 1994). The strong acidity of OA disrupts osmoregulation and causes uid accumulation in nematodes, destroying their cells, tissues, and eggs (Jang et al 2016;Seo and Kim 2014).…”

Section: Introductionmentioning

confidence: 99%

Control of A Tomato Plant Root-Knot Nematode By Induced Resistance Of Oxalic Acid Derived From Aspergillus Niger

Yeon

Park

MinKyu-Kang

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

Aspergillus niger F22 producing oxalic acid (OA) as a nematicidal component is currently used as a microbial nematicide. OA is known to induce systemic resistance in plant diseases caused by fungi, bacteria, and viruses, but the induced resistance of OA has not yet been elucidated in plant diseases caused by root-knot nematodes (RKNs). In this study, we investigated the functional mechanism of induced resistance of A. niger F22 formulation (Nemafree, 20% SC) and OA in tomato plant RKN disease caused by Meloidogyne incognita and analyzed their effectiveness against the disease. Foliar spray and soil drench treatments of Nemafree and OA were effective in the management of M. incognita in tomato plant in-pot experiments. When Nemafree and OA were applied 4 days before inoculation of M. incognita eggs, the treatments of Nemafree (4,000-fold dilution) and OA (0.22 mM) reduced root gall formation by more than 50%. The soil drench treatment also effectively suppressed RKN disease in field experiments. Moreover, the treatments of Nemafree and OA enhanced the transcriptional expression of pathogenesis-related 1 gene, plant proteinase inhibitor-II, and polyphenol oxidase genes and improved the production of total phenols, flavonoids, and lignin in the tomato plants infected with M. incognita. These results demonstrate that RKN diseases can be effectively controlled by induced resistance even at low concentrations of Nemafree or OA. Accordingly, our study provides evidence for more economical and efficient application strategies of microbial nematicides that control RKNs under field conditions.

show abstract

Biological Control of Root-Knot Nematodes by Organic Acid-Producing Lactobacillus brevis WiKim0069 Isolated from Kimchi

Cited by 28 publications

References 50 publications

Bioassay‐Guided Isolation of Nematicidal Artemisinic Acid and Dihydroartemisinic Acid from Artemisia annua L. and Evaluation of Their Activity against Meloidogyne incognita

Bioassay‐Guided Isolation of Nematicidal Artemisinic Acid and Dihydroartemisinic Acid from Artemisia annua L. and Evaluation of Their Activity against Meloidogyne incognita

Optimizing Safe Approaches to Manage Plant-Parasitic Nematodes

Control of A Tomato Plant Root-Knot Nematode By Induced Resistance Of Oxalic Acid Derived From Aspergillus Niger

Contact Info

Product

Resources

About