Biological control of Rhizoctonia solani in cowpea plants using yeast

Tenório, Dyana A. de; Medeiros, Érika Valente de; Lima, Cristiano Souza; Silva, Jéssica M. da; Barros, Jamilly Alves de; Neves, Rejane Pereira; Laranjeira, Delson

doi:10.1007/s40858-019-00275-2

Cited by 17 publications

(7 citation statements)

References 27 publications

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“…Bullera spp. have been known to function as biocontrol agents that improve disease resistance and growth in plants [ 56 ]. Therefore, the higher relative abundance of Bullera spp.…”

Section: Discussionmentioning

confidence: 99%

Wild rice harbors more root endophytic fungi than cultivated rice in the F1 offspring after crossbreeding

et al. 2021

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Background Rice, which serves as a staple food for more than half of the world’s population, is grown worldwide. The hybridization of wild and cultivated rice has enabled the incorporation of resistance to varying environmental conditions. Endophytic microbiota are known to be transferred with their host plants. Although some studies have reported on the endophytic microbiota of wild and cultivated rice, the inheritance from wild and cultivated rice accessions in next generations, in terms of endophytic microbiota, has not been examined. Results In the present study, the endophytic microbial community structures of Asian and African wild and cultivated rice species were compared with those of their F1 offspring. High-throughput sequencing data of bacterial 16S rDNA and fungal internal transcribed spacer regions were used to classify the endophytic microbiota of collected samples of rice. Results indicated that when either African or Asian wild rice species were crossed with cultivated rice accessions, the first generation harbored a greater number of root endophytic fungi than the cultivated parent used to make the crosses. Network analysis of the bacterial and fungal operational taxonomic units revealed that Asian and African wild rice species clustered together and exhibited a greater number of significant correlations between fungal taxa than cultivated rice. The core bacterial genus Acidovorax and the core fungal order Pleosporales, and genera Myrothecium and Bullera connected African and Asian wild rice accessions together, and both the wild rice accessions with their F1 offspring. On the other hand, the core bacterial genus Bradyrhizobium and the core fungal genera Dendroclathra linked the African and Asian cultivated rice accessions together. Conclusions This study has theoretical significance for understanding the effect of breeding on the inheritance of endophytic microbiota of rice and identifying beneficial endophytic bacteria and fungi among wild and cultivated rice species, and their F1 offspring.

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“…Bullera spp. have been known to function as biocontrol agents that improve disease resistance and growth in plants [ 56 ]. Therefore, the higher relative abundance of Bullera spp.…”

Section: Discussionmentioning

confidence: 99%

Wild rice harbors more root endophytic fungi than cultivated rice in the F1 offspring after crossbreeding

et al. 2021

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“…The microbiota has been correlated with such a suppressiveness of the soil, suggesting that beneficial microbes may play an important role in the management of Rhizoctonia diseases (Cordovez et al, 2015;Jambhulkar et al, 2015;Schlatter et al, 2017). Nevertheless, despite several experimental proofs (D'Aes et al, 2011;Huang et al, 2012;de Tenório et al, 2019;Wu et al, 2019), there are no examples of large-scale biological control of this pathogen. On the other hand, a spectacular example of biological control is the use of Phlebiopsis gigantea against Heterobasidion spp., which are both Basidiomycetes.…”

Section: Introductionmentioning

confidence: 99%

Basidiomycetes Are Particularly Sensitive to Bacterial Volatile Compounds: Mechanistic Insight Into the Case Study of Pseudomonas protegens Volatilome Against Heterobasidion abietinum

et al. 2021

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Volatile organic compounds (VOCs) play an important role in the communication among organisms, including plants, beneficial or pathogenic microbes, and pests. In vitro, we observed that the growth of seven out of eight Basidiomycete species tested was inhibited by the VOCs of the biocontrol agent Pseudomonas protegens strain CHA0. In the Ascomycota phylum, only some species were sensitive (e.g., Sclerotinia sclerotiorum, Botrytis cinerea, etc.) but others were resistant (e.g., Fusarium oxysporum f. sp. cubense, Verticillium dahliae, etc.). We further discovered that CHA0 as well as other ten beneficial or phytopathogenic bacterial strains were all able to inhibit Heterobasidion abietinum, which was used in this research as a model species. Moreover, such an inhibition occurred only when bacteria grew on media containing digested proteins like peptone or tryptone (e.g., Luria-Bertani agar or LBA). Also, the inhibition co-occurred with a pH increase of the agar medium where the fungus grew. Therefore, biogenic ammonia originating from protein degradation by bacteria was hypothesized to play a major role in fungus inhibition. Indeed, when tested as a synthetic compound, it was highly toxic to H. abietinum (effective concentration 50% or EC50 = 1.18 M; minimum inhibitory concentration or MIC = 2.14 M). Using gas chromatography coupled to mass spectrometry (GC/MS), eight VOCs were found specifically emitted by CHA0 grown on LBA compared to the bacterium grown on potato dextrose agar (PDA). Among them, two compounds were even more toxic than ammonia against H. abietinum: dimethyl trisulfide had EC50 = 0.02 M and MIC = 0.2 M, and 2-ethylhexanol had EC50 = 0.33 M and MIC = 0.77 M. The fungus growth inhibition was the result of severe cellular and sub-cellular alterations of hyphae occurring as early as 15 min of exposure to VOCs, as evidenced by transmission and scanning electron microscopy observations. Transcriptome reprogramming of H. abietinum induced by CHA0’s VOCs pointed out that detrimental effects occurred on ribosomes and protein synthesis while the cells tried to react by activating defense mechanisms, which required a lot of energy diverted from the growth and development (fitness cost).

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“…Quando mais velho o micélio, a coloração que pode ser bactérias (ex: Bacillus subtillis), fungos (ex: Trichoderma harzianum) ou leveduras (ex: Saccharomyces cerevisiae).As leveduras têm sido usadas como agentes de biocontrole e entre elas, a levedura do o gênero Saccharomyces tem se destacado(GOUVEA et al, 2007).Entretanto outros gêneros também tem sido estudados como Pichia spp., P. guilliermondii, Rhodotorula mucilaginosa que apresentaram eficiência no controle de doenças em pós-colheita(SILVA et al, 2015). As leveduras Candida saopaulonensis C6A, C. laurentii FVC10 e Bullera sinensis FVF10 (R1) foram testadas para o controle do tombamento e da podridão radicular causada por Rhizoctonia solani em plantas de feijão-caupi e reduziram a severidade da doença em 57,4%, 48,5% e 66,3%, respectivamente, sendo considerados promissores para atuarem como agentes de biocontrole(TENÓRIO et al, 2019).Outra levedura como potencial para ser usada no controle biológico é Lachancea (Kluyveromyces) thermotolerans, que é encontrada naturalmente em uvas, mas também em outros habitats como solo, insetos e plantas sendo amplamente distribuídas em todo o mundo(MORATA et al, 2018). Por apresentar esta versatilidade de habitats, pode mais facilmente se adaptar as diferentes ambiente e, possivelmente, atuar no controle biológico, embora não se conheça o seu modo de ação.Saab (2016), trabalhou com L. thermotolerans, em ensaio in vitro, para controle de Phythphotora sojae e observou formação de halo de inibição, sugerindo a produção de substância antibiótica e quando realizou bioensaios para detectar compostos voláteis, não verificou nenhum efeito sobre o fitopatógeno.As células microbianas, também podem ser utilizados para induzir a produção de fitoalexinas nas plantas.…”

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RESPOSTA DE VARIEDADE DE CULTIVO ORGÂNICO DE MILHO EM DIFERENTES FONTES DE ADUBO E INOCULAÇÃO DE Azospirillum brasilense

Peretto¹,

Amaral²

2020

Agricultura Em Bases Agroecológicas E Conservacionista

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Todo o conteúdo deste livro está licenciado sob uma Licença de Atribuição Creative Commons. Atribuição 4.0 Internacional (CC BY 4.0).O conteúdo dos artigos e seus dados em sua forma, correção e confiabilidade são de responsabilidade exclusiva dos autores. Permitido o download da obra e o compartilhamento desde que sejam atribuídos créditos aos autores, mas sem a possibilidade de alterá-la de nenhuma forma ou utilizá-la para fins comerciais.

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Biological control of Rhizoctonia solani in cowpea plants using yeast

Cited by 17 publications

References 27 publications

Wild rice harbors more root endophytic fungi than cultivated rice in the F1 offspring after crossbreeding

Wild rice harbors more root endophytic fungi than cultivated rice in the F1 offspring after crossbreeding

Basidiomycetes Are Particularly Sensitive to Bacterial Volatile Compounds: Mechanistic Insight Into the Case Study of Pseudomonas protegens Volatilome Against Heterobasidion abietinum

RESPOSTA DE VARIEDADE DE CULTIVO ORGÂNICO DE MILHO EM DIFERENTES FONTES DE ADUBO E INOCULAÇÃO DE Azospirillum brasilense

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