Biological control of Ralstonia wilt, Phytophthora blight, Meloidogyne root-knot on bell pepper by the combination of Bacillus subtilis AR12, Bacillus subtilis SM21 and Chryseobacterium sp. R89

Liu, Hongxia; Li, Shimo; Luo, Yuming; Luo, Laixin; Li, Jianqiang; Guo, Jianhua

doi:10.1007/s10658-013-0369-2

Cited by 44 publications

(18 citation statements)

References 25 publications

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“…), especially 150 d after treatment. This enhancement of disease control can be partially due to an additive effect as both beneficial microbe and SMS are effective in disease control ( Liu et al., ; Kwak et al., ). Moreover, the enhanced biocontrol efficiency was also possibly attributed to synergistic disease suppression acquired with a rapid and long‐lasting beneficial bacterial growth and colonization supported by the enriched nutrition and appropriate humidity from the agro‐industry waste in the BF+organic fertilizers combination.…”

Section: Discussionmentioning

confidence: 99%

“…One of the major cash crops in China, pepper ( Capsicum annuum L.), like other solanaceous vegetables, suffers a wide range of soil‐borne diseases including bacterial wilt, Phytophthora blight, and root‐knot nematode disease caused by Ralstonia solanacearum, Phytophthora capsici , and Meloidogyne spp., respectively ( Zhou et al., ; Barbary et al., ; Du et al., ). In agriculture practice, soil‐borne diseases tend to occur in a mixed pattern, causing severe economic losses ( Liu et al., ). The rising concerns on the risk of food safety, environmental pollution, and pathogen resistance as well as increasing policy demand for reduction of chemical fertilizers and biocides require alternatives in nutrient application and disease management.…”

Section: Introductionmentioning

confidence: 99%

“…Efforts have been devoted to screening for PGPR with biological control ability and seeking for more efficient technologies in application in agricultural practice. Selecting consortia of mixed PGPR ( Liu et al., ) with additive or synergistic effects is one of the attempts to achieve more stable and effective biocontrol efficiencies.…”

Section: Introductionmentioning

confidence: 99%

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Combination of agricultural waste compost and biofertilizer improves yield and enhances the sustainability of a pepper field

Qiu

et al. 2019

J. Plant Nutr. Soil Sci.

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Application of plant growth‐promoting rhizobacteria (PGPR) has been considered as an environmentally friendly method for crop yield promotion as well as plant disease management. Efforts have been devoted to unraveling mechanisms involved in bacteria–plant and bacteria–pathogen interactions. However, little is known on the effect of the interaction among PGPR, soil, and plant. We compared growth and yield promotion capacity of biofertilizer Ning Shield, a consortium of bacterial preparation used as a biofertilizer (BF), and its mixture with compost of agricultural waste including spent substrate of Pleurotus ostreatus (SSP)/Volvariella volvacea (SSV), chicken manure (CM), and inorganic fertilizer (IOF) in a pepper field, respectively. The disease control efficacy, pepper fruit preservation time, and nutrients were also determined. Soil nutrient parameters including organic matter and available NPK of treatments were assayed before and after one growth season. All of the mixture of BF+organic compost treatment significantly enhanced the yield and quality of pepper fruit. Moreover, disease control capacity was promoted by the mixture of BF+organic compost, with BF+SSV reaching the highest control efficacy of 81% on 60th day after transplanting, and remaining 76% at the 105th day. The BF+SSV treatment showed soil fertility retention ability with higher soil nutrient contents after one growth season of pepper. This study provides evidence that, when combined with organic fertilizers such as spent mushroom substrate compost, beneficial microbes have the ability to promote plant growth and yield as well as suppress plant disease by sustaining soil fertility through complex bacteria–soil–plant interaction.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Combination of agricultural waste compost and biofertilizer improves yield and enhances the sustainability of a pepper field

Qiu

et al. 2019

J. Plant Nutr. Soil Sci.

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“…Trichoderma harzianum 10,11 , Trichoderma viride 12 , Trichoderma asperellum 13 , Trichoderma virens 14 , Pseudomonas fluorescence 15,16 and Bacillus subtilis 17 are used as biocontrol agents to control bacterial wilt. Combination treatment methods using two or more of these agents are more effective in managing the disease than treatment using a single biocontrol agent 10,18,19 . Chemical bactericides and fungicides induce resistance in pathogens during long term use, which ultimately makes the pathogen tolerant to these chemical applications [20][21][22][23] .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Trichoderma spp., Pseudomonas fluorescence and Bacillus subtilis for biological control of Ralstonia wilt of tomato

Yendyo

G.C.²,

Pandey³

2017

F1000Res

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Abstractis the major bacterial disease in tomato, Background: Ralstonia solanacearum which invades the roots of diverse plant hosts and colonizes xylem vessels causing wilt, especially in tropical, subtropical and warm-temperate regions. R.produces several toxins helping it to invade the plant's natural solanacearum defense mechanism. Native isolates of Trichoderma spp., Pseudomonas and can be used as biocontrol agents to control fluorescence Bacillus subtilis the bacterial wilt and combined application of these beneficial microbes can give better results.Bacterial wilt infection in the field was identified by field experts and Methods: the infected plant part was used to isolate in CPG Ralstonia solanacearum media and was positively identified. Subsequently, the efficacy of the biocontrol agents was tested and documented using agar well diffusion technique and digital microscopy. 2ml of the microbial concentrate (10 cfu/ml) was mixed in one liter of water and was applied in the plant root at the rate of 100 ml per plant as a treatment method.It was observed that the isolated spp. AA2 and Results: Trichoderma PFS were most potent in inhibiting the growth of Pseudomonas fluorescence R. showing ZOI 20.67 mm and 22.33 mm, respectively. Digital solanacearum, microscopy showed distinct inhibitory effect on the growth and survival of R.The results from the field data indicated that spp. solanacearum. Trichoderma and alone were able to prevent 92% and 96% of Pseudomonas fluorescence the infection and combination of both were more effective, preventing 97% of infection. Chemical control methods prevented 94% of infection.Results showed the bio-efficacy of the native isolates. The Conclusions: various level of antagonistic effect against shown by all 13 R. solanacearum isolates, including the results shown by native isolates in the field, manifested the promising potential of the biocontrol agents, like and Trichoderma against controlling the bacterial wilt infection. Pseudomonas

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“…Trichoderma harzianum 10, 11 , Trichoderma viride 12 , Trichoderma asperellum 13 , Trichoderma virens 14 , Pseudomonas fluorescens 15, 16 and Bacillus subtilis 17 are used as biocontrol agents to control bacterial wilt. Combination treatment methods using two or more of these agents are more effective in managing the disease than treatment using a single biocontrol agent 10, 18, 19 . Chemical bactericides and fungicides induce resistance in pathogens during long-term use, which ultimately makes the pathogen tolerant to these chemical applications 20– 23 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Trichoderma spp., Pseudomonas fluorescens and Bacillus subtilis for biological control of Ralstonia wilt of tomato

Yendyo

G.C.²,

Pandey³

2018

F1000Res

View full text Add to dashboard Cite

Background: Ralstonia spp. is a major pathogenic microbe for tomato, which invades the roots of diverse plant hosts and colonizes xylem vessels causing wilt, especially in tropical, subtropical and warm-temperate regions. Ralstonia spp. produces several virulence factors helping it to invade the plant’s natural defense mechanism. Native isolates of Trichoderma spp., Pseudomonas fluorescens and Bacillus subtilis can be used as biocontrol agents to control the bacterial wilt and combined application of these beneficial microbes can give better results. Methods: Bacterial wilt infection in the field was identified by field experts and the infected plant part was used to isolate Ralstonia spp. in CPG media and was positively identified. Subsequently, the efficacy of the biocontrol agents was tested and documented using agar well diffusion technique and digital microscopy. 2ml of the microbial concentrate (10 9 cells/ml) was mixed in one liter of water and was applied in the plant root at the rate of 100 ml per plant as a treatment method. Results: It was observed that the isolated Trichoderma spp. AA2 and Pseudomonas fluorescens PFS were most potent in inhibiting the growth of Ralstonia spp. , showing ZOI 20.67 mm and 22.33 mm, respectively. Digital microscopy showed distinct inhibitory effect on the growth and survival of Ralstonia spp . The results from the field data indicated that Trichoderma spp. and Pseudomonas fluorescens alone were able to prevent 92% and 96% of the infection and combination of both were more effective, preventing 97% of infection. Chemical control methods prevented 94% of infection. Bacillus subtilis could only prevent 84 % of the infection. Conclusions: Antagonistic effect against Ralstonia spp. shown by native isolates of Trichoderma spp. and P. fluorescens manifested the promising potential as biocontrol agents. Combined application gave better results. Results shown by Bacillus subtilis were not significant.

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Biological control of Ralstonia wilt, Phytophthora blight, Meloidogyne root-knot on bell pepper by the combination of Bacillus subtilis AR12, Bacillus subtilis SM21 and Chryseobacterium sp. R89

Cited by 44 publications

References 25 publications

Combination of agricultural waste compost and biofertilizer improves yield and enhances the sustainability of a pepper field

Combination of agricultural waste compost and biofertilizer improves yield and enhances the sustainability of a pepper field

Evaluation of Trichoderma spp., Pseudomonas fluorescence and Bacillus subtilis for biological control of Ralstonia wilt of tomato

Evaluation of Trichoderma spp., Pseudomonas fluorescens and Bacillus subtilis for biological control of Ralstonia wilt of tomato

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