Biological control of Pythium aphanidermatum in cucumber with a combined application of Lysobacter enzymogenes strain 3.1T8 and chitosan

Postma, J.; Stevens, Luc; Wiegers, G.L.; Davelaar, E.; Nijhuis, E.H.

doi:10.1016/j.biocontrol.2008.11.006

Cited by 64 publications

(60 citation statements)

References 32 publications

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“…have been shown high biocontrol potentials against a number of fugal and peronosporomycetal plant diseases Zhang and Yuen 1999;Islam et al 2004aIslam et al , 2005bJi et al 2008;Postma et al 2009). However, effect of root colonization of Lysobacter spp.…”

Section: Discussionmentioning

confidence: 97%

“…have been reported as potential candidates for biocontrol of fungal and Peronosporomycete phytopathogens Zhang and Yuen 1999;Zhang and Yuen 2000;Yuen and Zhang 2001;Zhang et al 2001;Islam et al 2005a, b;Kobayashi et al 2005;Yu et al 2007). Several new species of Lysobacter have recently been reported with high promise for biocontrol activity against fungal and peronosporomycete phytopathogens (Park et al 2008;Ji et al 2008;Postma et al 2008Postma et al , 2009). However, the mode of action of antagonisms of these potential biocontrol agents against phytopathogens is poorly understood.…”

Section: Introductionmentioning

confidence: 94%

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Mode of antagonism of a biocontrol bacterium Lysobacter sp. SB-K88 toward a damping-off pathogen Aphanomyces cochlioides

Islam

2009

World J Microbiol Biotechnol

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The biocontrol bacterium Lysobacter sp. SB-K88 suppresses damping-off disease in sugar beet and spinach caused by Aphanomyces cochlioides and Pythium sp. through characteristic plant colonization and antibiosis against the pathogens. This study aimed to unravel further details on mode of antagonism of SB-K88 against a damping-off pathogen A. cochlioides AC-5. The SB-K88 substantially inhibited growth and decomposed AC-5 mycelia and suppressed the release of zoospores from the hyphae. The excised root tips of sugar beet seedlings from seeds previously inoculated with SB-K88 were less attractive to AC-5 zoospores. Although aerial growth was not affected, however, root hairs of SB-K88 inoculated sugar beet seedlings were remarkably shorter and thicker than those of uninoculated control. When exposed to zoospores, the SB-K88 inhibited motility of zoospores and/or caused lysis, and then aggregated around the dead cystospores or lysed residues within 3-6 h likely to be micro-predatory behavior to a eukaryotic organism. Confocal laser scanning microscopic analysis revealed that number of lipid bodies and activities of mitochondria were markedly increased in the affected hyphae compared with control hyphae as visualized by established vital stains. Taken together, these results suggest that Lysobacter sp. SB-K88 suppresses damping-off diseases through exerting multifaceted antagonistic effects against the peronosporomycetes.

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Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 94%

Mode of antagonism of a biocontrol bacterium Lysobacter sp. SB-K88 toward a damping-off pathogen Aphanomyces cochlioides

Islam

2009

World J Microbiol Biotechnol

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“…One of the factors to be considered is hyphal walls which are composed of 80-90% polysaccharides, mainly β1-6 linked glucans and β1-3 and β1-4 [5]. It should be noted that Pythium spp.…”

Section: Introductionmentioning

confidence: 99%

A Rapid Primary Screening Method for Antitumor Using the Oomycete Pythium aphanidermatum

Santos¹,

Melo²

2016

Nat Prod Chem Res

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Investigating bioactive components from microbial metabolites can provide potential source for drug discovery. There are a number of traditional antitumor compounds screening assays already described. In this study, we have developed an efficient method to search for antitumor compounds using the Oomycete Pythium aphanidermatum as model on agar plates for high throughtput screening. Antioomycete compounds showed cytotoxic effects in human cancer cells lines due to the possible similarity between the lipid cell membrane of the Pythium species and cancerous cells, suggesting the compounds act through similar mechanisms of inhibition. In this assay, it is possible to evaluate hundreds of samples, with results in few hours. The Pythium assay was shown to be sensitive for several chemical substances and allowed the detection and isolation of antitumor compounds from microorganisms. This bioassay was shown to be sensitive, rapid, simple and reliable for antitumor activity. It takes just few hours to evaluate the properties of the extracts.

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“…Anovel antifungal compound (HSAF, 1) was isolated from L. enzymogenes C3 and can disrupt sphingolipids important to the polarized growth of filamentous fungi, thus, L. enzymogenes C3 was used in the biological control of fungal diseases of plants (Lou et al, 2011). L enzymogenes OH11 secretes chitinases that hydrolyzed the pathogenic fungal cell wall contributing to suppression of proliferation (Postma et al, 2009); Lysobacter sp. strain sbk88 and L. enzymogenes 3.1t8 can produce antibiotics that are involved in suppression of Pythium aphanidermatum and Aphanomyces cochlioides (Kato et al, 1998).…”

Section: Author(s) Agree That This Article Remains Permanently Open Amentioning

confidence: 99%

Genetic diversity analysis of rep-PCR genomic fingerprinting of Lysobacter spp.

Fu¹,

Zhang²,

Zhang³

et al. 2016

Afr. J. Microbiol. Res.

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Biological control of Pythium aphanidermatum in cucumber with a combined application of Lysobacter enzymogenes strain 3.1T8 and chitosan

Cited by 64 publications

References 32 publications

Mode of antagonism of a biocontrol bacterium Lysobacter sp. SB-K88 toward a damping-off pathogen Aphanomyces cochlioides

Mode of antagonism of a biocontrol bacterium Lysobacter sp. SB-K88 toward a damping-off pathogen Aphanomyces cochlioides

A Rapid Primary Screening Method for Antitumor Using the Oomycete Pythium aphanidermatum

Genetic diversity analysis of rep-PCR genomic fingerprinting of Lysobacter spp.

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