Biological Control of Peach Fungal Pathogens by Commercial Products and Indigenous Yeasts

Restuccia, Cristina; Giusino, Francesco; Licciardello, Fabio; Randazzo, Cinzia Lucia; Caggia, Cinzia; Muratore, Giuseppe

doi:10.4315/0362-028x-69.10.2465

Cited by 37 publications

(22 citation statements)

References 29 publications

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“…Pichia kluyveri and related species (e.g., Wickerhamomyces anomalus , P. fermentans , etc.) are widely studied with respect to wine fermentation as well as biological control, mostly of fungal postharvest diseases of fruits [30, 96–101]. …”

Section: Discussionmentioning

confidence: 99%

Competition assays and physiological experiments of soil and phyllosphere yeasts identify Candida subhashii as a novel antagonist of filamentous fungi

et al. 2017

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BackgroundWhile recent advances in next generation sequencing technologies have enabled researchers to readily identify countless microbial species in soil, rhizosphere, and phyllosphere microbiomes, the biological functions of the majority of these species are unknown. Functional studies are therefore urgently needed in order to characterize the plethora of microorganisms that are being identified and to point out species that may be used for biotechnology or plant protection. Here, we used a dual culture assay and growth analyses to characterise yeasts (40 different isolates) and their antagonistic effect on 16 filamentous fungi; comprising plant pathogens, antagonists, and saprophytes.ResultsOverall, this competition screen of 640 pairwise combinations revealed a broad range of outcomes, ranging from small stimulatory effects of some yeasts up to a growth inhibition of more than 80% by individual species. On average, yeasts isolated from soil suppressed filamentous fungi more strongly than phyllosphere yeasts and the antagonistic activity was a species-/isolate-specific property and not dependent on the filamentous fungus a yeast was interacting with. The isolates with the strongest antagonistic activity were Metschnikowia pulcherrima, Hanseniaspora sp., Cyberlindnera sargentensis, Aureobasidium pullulans, Candida subhashii, and Pichia kluyveri. Among these, the soil yeasts (C. sargentensis, A. pullulans, C. subhashii) assimilated and/or oxidized more di-, tri- and tetrasaccharides and organic acids than yeasts from the phyllosphere. Only the two yeasts C. subhashii and M. pulcherrima were able to grow with N-acetyl-glucosamine as carbon source.ConclusionsThe competition assays and physiological experiments described here identified known antagonists that have been implicated in the biological control of plant pathogenic fungi in the past, but also little characterised species such as C. subhashii. Overall, soil yeasts were more antagonistic and metabolically versatile than yeasts from the phyllosphere. Noteworthy was the strong antagonistic activity of the soil yeast C. subhashii, which had so far only been described from a clinical sample and not been studied with respect to biocontrol. Based on binary competition assays and growth analyses (e.g., on different carbon sources, growth in root exudates), C. subhashii was identified as a competitive and antagonistic soil yeast with potential as a novel biocontrol agent against plant pathogenic fungi.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-016-0908-z) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Competition assays and physiological experiments of soil and phyllosphere yeasts identify Candida subhashii as a novel antagonist of filamentous fungi

et al. 2017

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“…USDA Fungus-Host Database reports 375 cases of Mucor infections from plants from approximately 40 countries in Europe, Central and South-East Asia, Australia, Africa, and North and South America (Farr and Rossman 2014). Mucor circinelloides causes rots in tomatoes (Smith et al 1976), mangoes (Johnson 2008), yam (Amusa et al 2003) and peaches (Restuccia et al 2006). Mucor hiemalis can be pathogenic on guavas (Kunimoto et al 1977), carrots and cassava (Snowdon 1991).…”

Section: Introductionmentioning

confidence: 99%

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

et al. 2014

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Many fungi are pathogenic on plants and cause significant damage in agriculture and forestry. They are also part of the natural ecosystem and may play a role in regulating plant numbers/density. Morphological identification and analysis of plant pathogenic fungi, while important, is often hampered by the scarcity of discriminatory taxonomic characters and the endophytic or inconspicuous nature of these fungi. Molecular (DNA sequence) data for plant pathogenic fungi have emerged as key information for diagnostic and classification studies, although hampered in part by non-standard laboratory practices and analytical methods. To facilitate current and future research, this study provides phylogenetic synopses for 25 groups of plant pathogenic fungi in the Ascomycota, Basidiomycota, Mucormycotina (Fungi), and Oomycota, using recent molecular data, up-to-date names, and the latest taxonomic insights. Lineagespecific laboratory protocols together with advice on their application, as well as general observations, are also provided. We hope to maintain updated backbone trees of these fungal lineages over time and to publish them jointly as new data emerge. Researchers of plant pathogenic fungi not covered by the present study are invited to join this future effort. Bipolaris, Botryosphaeriaceae,

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“…Late Peach of Leonforte) (Restuccia et al 2006). They recorded 100% disease incidence after 15 days in a biological control experiment.…”

Section: Discussionmentioning

confidence: 99%

“…persica) (Restuccia et al 2006) and Penicillium chrysogenum Thom on blackplum (plum-like fruit of Vitex doniana Nielson) (Eseigbe and Bankole 1996). Lesions caused by P. chrysogenum on blackplum were small (≥8mm) after 8 days incubation (28±2ºC) and the fungus was infrequently isolated (19%) from the rotten fruit.…”

Section: Other Reportedmentioning

confidence: 99%

Postharvest decay of nectarine and plum caused by Penicillium spp.

Louw

Korsten

2016

Eur J Plant Pathol

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Stone fruit are highly perishable and susceptible to numerous postharvest pathogens. P. expansum is a well-known pathogen of stone fruit but little is known about other Penicillium spp. that could potentially cause decay. This study aims to determine pathogenicity profiles of P. expansum, P. crustosum, P. solitum and P. digitatum on selected nectarine and plum cultivars, and in part examine the disease cycle within new fruit-Penicillium interactions to observe the potential of the pathogens to cross-infect. Lesions caused by Penicillium spp.isolated from the pear and citrus handling chain environments were not different on nectarine.P. digitatum was the most aggressive species on most nectarines and plums evaluated. Decay was associated with older fruit (long stored). The highest aggression was observed on Nectargold, May Glo and African Rose. P. expansum and P. crustosum had the highest disease incidences and were the second and third most aggressive species respectively. P. solitum caused small lesions. Its role in the fresh produce market can be negligible.Scanning electron microscopy confirmed infection and provided new information on the growth and reproduction of P. expansum, P. crustosum and P. digitatum on infected nectarine, pear and lemon. Pear and lemon can serve as cross-infection sources for stone fruit in the fresh produce chain. To our knowledge this is the most complete description of disease Postharvest decay of nectarine and plum caused by Penicillium spp.2 caused by P. digitatum, P. crustosum and P. solitum on nectarine and plum. Rapid decay caused by P. digitatum highlighted the potential of the species to contribute to losses in the stone fruit industry. Future research should investigate the presence and impact of P. digitatum in the stone fruit supply chain. The role of fruit maturity in fruit-Penicillium interactions requires further investigation.

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Biological Control of Peach Fungal Pathogens by Commercial Products and Indigenous Yeasts

Cited by 37 publications

References 29 publications

Competition assays and physiological experiments of soil and phyllosphere yeasts identify Candida subhashii as a novel antagonist of filamentous fungi

Competition assays and physiological experiments of soil and phyllosphere yeasts identify Candida subhashii as a novel antagonist of filamentous fungi

One stop shop: backbones trees for important phytopathogenic genera: I (2014)

Postharvest decay of nectarine and plum caused by Penicillium spp.

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