Biological control of fusarium crown and root rot of tomato with antagonistic bacteria and integrated control when combined with the fungicide carbendazim

Abstract

Two bacterial isolates, Bacillus megaterium (c96) and Burkholderia cepacia (c91), demonstrated to be antagonistic against Fusarium oxysporum f.sp. radicis-lycopersici , the causal organism of fusarium crown and root rot of tomato, were evaluated as biocontrol agents alone and when integrated with the fungicide carbendazim. In an initial screening, these isolates reduced disease incidence by 75 and 88%, respectively. In vitro , both biocontrol agents were highly tolerant to the fungicide carbendazim, commonly used to control fusarium diseases. Carbendazim reduced disease symptoms by over 50% when used at > 50 µ g mL − 1 , but had little effect at lower concentrations. Combination of the bacterial isolates and carbendazim gave significant ( P ≤ 0·05) control of the disease when plants were artificially inoculated with the pathogen. Application of carbendazim at a low concentration (1 µ g mL − 1 ) in combination with B. cepacia c91 reduced disease symptoms by 46%, compared with a reduction of 20% obtained with the bacterium alone and no control with the chemical treatment alone. A combination of B. megaterium c96 with an increased application rate of 10 µ g mL − 1 carbendazim significantly reduced disease symptoms by 84% compared with inoculated controls and by 77% compared with carbendazim treatment alone. In this experiment, the integrated treatment also slightly outperformed application of 100 µ g mL − 1 carbendazim, and bacteria applied without fungicide also provided good disease control.

Application of antagonistic rhizobacteria for control of Fusarium seedling blight and basal rot of lily

“…Therefore, this combined treatment method would be more practical as it is more environmentally friendly than the current treatment of Sporgon at 400 μg/mL alone. The finding in this study confirms previous reports that combined treatments of biocontrol agents and a reduced rate of fungicides are effective for control of soilborne pathogens in various crops (Zhou and Reeleder 1990;Chand-Goyal and Spotts 1996;Conway et al 1997;Budge and Whipps 2001;Omar et al 2006). Pham et al (2004) reported that pesticides may cause specific stresses on bacterial cells such as damage to proteins, oxidative DNA, or cell membranes.…”

Evaluation of Selected Bacterial Endophytes for Biocontrol Potential against Phytophthora Blight of Bell Pepper (Capsicum annuum L.)

“…The potential of including BCAs with mefenoxam can be in form of rotations with mefenoxam to reduce the frequency of fungicide applications for a more environmentally friendly, long lasting, consistent, and effective control of P. capsici. Similar results on BCA have been reported in other studies [50][51][52][53][54].…”

Efficacy of Fungicide and Bio-Control Agents against Root Rot of Black Gram (Vigna mungo L.) caused by Macrophomina phaseolina (Tassi) Goid

“…Percentage inhibition for 0.1% concentration is 93 per cent ( Table 1). The results are in accordance with the previous work of López-Herrera et al, (2007) and Omar et al, (2006) In vitro effect of fungal and bacterial antagonist against M. phaseolina (Dual culture technique)…”