Biological control of chestnut blight in Croatia: an interaction between host sweet chestnut, its pathogen <i>Cryphonectria parasitica</i> and the biocontrol agent <i>Cryphonectria hypovirus</i> 1

Krstin, Ljiljana; Katanić, Zorana; Ježić, Marin; Poljak, Igor; Nuskern, Lucija; Matković, Ivana; Idžojtić, Marilena; Ćurković‐Perica, Mirna

doi:10.1002/ps.4335

Cited by 23 publications

(21 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…There have been relatively few studies on the impact of CHV‐1 induced hypovirulence using plant material . To our knowledge, this is the first time that the infectivity of virulent and hypovirulent isolates of C. parasitica has been evaluated on chestnut seedlings although other studies have used cut chestnut stems, naturally infected trees or both .…”

Section: Discussionmentioning

confidence: 99%

“…Following discovery of its biocontrol potential in Italy in the late 1950s, hypovirulence began to be used routinely for disease control . Since then, many studies have identified various mycoviruses that could be useful tools for chestnut blight control in North America such as CHV‐1, CHV‐2, CHV‐3 and CHV‐4 although the latter does not cause hypovirulence, as well as CpMyRV‐1, or CpMyRV2, CHV‐1 and CHV‐2 found in Asia, and CHV‐1 in Europe . Hogan and Griffin showed evidence of the spread of CHV‐1 among local VCGs of the fungus after introduction from Europe into Virginia; and Zamora et al .…”

Section: Introductionmentioning

confidence: 99%

“…Other researchers demonstrated that some subtypes of CHV‐1 (e.g. subtype I, also known as Italian subtype) were just as damaging to isolates of C. parasitica as the a priori strongest F1 subtype …”

Section: Introductionmentioning

confidence: 99%

“…11 Since then, many studies have identified various mycoviruses that could be useful tools for chestnut blight control in North America such as CHV-1, 12-15 CHV-2, 16 CHV-3 17 and CHV-4 although the latter does not cause hypovirulence, 18 as well as CpMyRV-1, 19 or CpMyRV2, 20 CHV-1 and CHV-2 found in Asia, 21 and CHV-1 in Europe. 22,23 Hogan and Griffin 12 showed evidence of the spread of CHV-1 among local VCGs of the fungus after introduction from Europe into Virginia; and Zamora et al 22 also demonstrated that the effectiveness of subtype F1 (commonly known as French subtype) in north-western Spain was dependent on the fungus VCG. Other researchers demonstrated that some subtypes of CHV-1 (e.g.…”

Section: Introductionmentioning

confidence: 99%

“…subtype I, also known as Italian subtype) were just as damaging to isolates of C. parasitica as the a priori strongest F1 subtype. 23 During screening of > 200 C. parasitica isolates from the UK outbreak, CHV-1 was initially detected at very low frequency (3.5%) and concentration (14.7 ± 11.3 ng μL −1 ) in affected individuals. 6 These initial findings suggested that because the CHV-1 virus was already present in UK populations of C. parasitica it might be harnessed for disease control.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

Hypovirulent effect of theCryphonectria hypovirus 1in British isolates ofCryphonectria parasitica

Romon-Ochoa

Gorton

Lewis

et al. 2019

Pest Management Science

View full text Add to dashboard Cite

Background Chestnut blight, caused by Cryphonectria parasitica, is controlled in many European countries by the naturally occurring mycovirus Cryphonectria hypovirus 1 (CHV‐1). During surveys of recently identified chestnut blight outbreak in England, CHV‐1 was detected in several individuals of the pathogen isolated from affected trees. We investigated two of these CHV‐1‐infected isolates (L‐6 and Db‐1) as potential biocontrol agents for deployment in the UK comparing their virulence against virus‐free (M1275) and hypovirulent (M784) European isolates by inoculating sweet chestnut seedlings. Results Both the European CHV‐1 M784 hypovirulent isolate and UK L‐6 isolate formed significantly smaller lesions in sweet chestnut seedling bark than the other three isolates (Db‐1, and virulent isolates FTC121 and M1275). The highest virus concentration was detected in isolate M784, followed by L‐6, with the lowest concentration in isolate Db‐1. White colony colouration indicative of hypovirulence was common in colonies re‐isolated from smaller lesions, and the same isolates also tended to be slower growing in culture, have a higher virus concentration, and caused less epicormic growth and fewer stromata to be present in plants. L‐6 and Db‐1 virus sequences, respectively, matched the virus haplotype E‐5 detected previously in Switzerland and a mutation of the same subtype I haplotype. Conclusion Isolate L‐6 could potentially act as biocontrol for chestnut blight outbreaks in the UK but further laboratory and field experiments are needed. © 2019 Crown copyright. Pest Management Science © 2019 Society of Chemical Industry

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Hypovirulent effect of theCryphonectria hypovirus 1in British isolates ofCryphonectria parasitica

Romon-Ochoa

Gorton

Lewis

et al. 2019

Pest Management Science

View full text Add to dashboard Cite

show abstract

Mycoviruses as Antivirulence Elements of Fungal Pathogens

Ćurković‐Perica

Ježić

Rigling

2022

The Biological Role of a Virus

View full text Add to dashboard Cite

Cryphonectria hypovirus 1-Induced Changes of Stress Enzyme Activity in Transfected Phytopathogenic Fungus Cryphonectria parasitica

et al. 2017

View full text Add to dashboard Cite

Cryphonectria parasitica is a phytopathogenic fungus introduced from Eastern Asia to North America and to Europe, where it causes chestnut blight, a devastating disease of chestnut trees. The disease can be biologically controlled utilising the mycovirus Cryphonectria hypovirus 1 (CHV1), which changes the physiology of the host, reducing its virulence towards chestnut. We measured fungal growth in vitro and activities of glutathione S-transferase, catalase and superoxide dismutase, enzymes involved in oxidative stress response, to elucidate the effects of CHV1 infection on the host. Six CHV1 strains of different subtypes and three fungal isolates were used in different combinations to better represent natural conditions, where higher genetic diversity of both fungus and virus is expected. The infection with different CHV1 strains decreased in vitro growth rate of infected fungal isolates and increased activity of their stress enzymes in most of the studied fungus/virus combinations, indicating increased oxidative stress following CHV1 infection. All our field CHV1 strains belong to the Italian subtype, but while strain M56-1 had equal or even stronger effect on its fungal host than prototypic strain EP713 of French subtype F1, strain B11 had no effect. Thus, the severity of the observed effects depended on a particular virus strain, fungal isolate, and the combination of the two, rather than solely on the virus subtype. Since previous research showed discordance between accumulation of mRNA and stress-related proteins in CHV1 infected C. parasitica, our results emphasise the importance of enzymes' activity measurements as an invaluable extension of transcriptomic and proteomic analyses.

show abstract

Biological control of chestnut blight in Croatia: an interaction between host sweet chestnut, its pathogen Cryphonectria parasitica and the biocontrol agent Cryphonectria hypovirus 1

Cited by 23 publications

References 40 publications

Hypovirulent effect of theCryphonectria hypovirus 1in British isolates ofCryphonectria parasitica

Hypovirulent effect of theCryphonectria hypovirus 1in British isolates ofCryphonectria parasitica

Mycoviruses as Antivirulence Elements of Fungal Pathogens

Cryphonectria hypovirus 1-Induced Changes of Stress Enzyme Activity in Transfected Phytopathogenic Fungus Cryphonectria parasitica

Contact Info

Product

Resources

About