Biological control of bacterial soft rot in Chinese cabbage by Lactobacillus plantarum strain BY under field conditions

Tsuda, Ken; Tsuji, Gento; Higashiyama, Miyako; Ogiyama, Hiroshi; Umemura, Kenji; Mitomi, Masaaki; Kubo, Yasuyuki; Kosaka, Yoshitaka

doi:10.1016/j.biocontrol.2016.05.010

Cited by 53 publications

(41 citation statements)

References 23 publications

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“…Although after spraying a decrease in the population was observed as a result of the harsh conditions on aerial plant surfaces, a constant level of 10 3 to 10 4 CFU leaf −1 of LAB strains was attained in the 10 days that followed. This decline in population on leaves was also reported for other L. plantarum BCA which, interestingly, attained higher population levels at the wounded sites of leaves (Tsuda et al, ). Changes in water availability and temperature, nutrient limitation or ultraviolet radiation on leaves can be transiently inadequate for BCA growth (Lindow & Brandl, ).…”

Section: Discussionsupporting

confidence: 77%

“…This strategy has been previously used to screen for BCA in other pathosystems (Haidar et al, ). As some LAB strains have shown antagonistic activity against bacterial and fungal plant pathogens (Fhoula et al, ; Trias, Bañeras, Montesinos, & Badosa, ; Visser et al, ; Wang et al, ) and biocontrol efficacy on plants (Roselló et al, ; Roselló et al, ; Shrestha, Kim, & Park, ; Tsuda et al, ), plant‐associated LAB have been considered as candidates for developing BCA.…”

Section: Discussionmentioning

confidence: 99%

“…LAB have been widely reported as being biopreservatives of vegetables and fruits, inhibiting the growth of foodborne bacterial pathogens and spoilage fungi (Crowley, Mahony, & Van Sinderen, ; Trias, Bañeras, Badosa, & Montesinos, ; Trias, Bañeras, Montesinos, & Badosa, ). In addition, some LAB strains have also been reported as being potential BCA against several bacterial plant pathogens (Roselló et al, ; Tsuda et al, ; Visser, Holzapfel, Bezuidenhout, & Kotze, ).…”

Section: Introductionmentioning

confidence: 99%

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Biological control of bacterial plant diseases with Lactobacillus plantarum strains selected for their broad‐spectrum activity

Daranas

Roselló

Cabrefiga

et al. 2018

Annals of Applied Biology

100

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The use of lactic acid bacteria (LAB) to control multiple pathogens that affect different crops was studied, namely, Pseudomonas syringae pv. actinidiae in kiwifruit, Xanthomonas arboricola pv. pruni in Prunus and Xanthomonas fragariae in strawberry. A screening procedure based on in vitro and in planta assays of the three bacterial pathogens was successful in selecting potential LAB strains as biological control agents. The antagonistic activity of 55 strains was first tested in vitro and the strains Lactobacillus plantarum CC100, PM411 and TC92, and Leuconostoc mesenteroides CM160 and CM209 were selected because of their broad‐spectrum activity. The biocontrol efficacy of the selected strains was assessed using a multiple‐pathosystem approach in greenhouse conditions. L. plantarum PM411 and TC92 prevented all three pathogens from infecting their corresponding plant hosts. In addition, the biocontrol performance of PM411 and TC92 was comparable to the reference products (Bacillus amyloliquefaciens D747, Bacillus subtilis QST713, chitosan, acibenzolar‐S‐methyl, copper and kasugamycin) in semi‐field and field experiments. The in vitro inhibitory mechanism of PM411 and TC92 is based, at least in part, on a pH lowering effect and the production of lactic acid. Moreover, both strains showed similar survival rates on leaf surfaces. PM411 and TC92 can easily be distinguished because of their different multilocus sequence typing and random amplified polymorphic DNA profiles.

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Section: Discussionsupporting

confidence: 77%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Biological control of bacterial plant diseases with Lactobacillus plantarum strains selected for their broad‐spectrum activity

Daranas

Roselló

Cabrefiga

et al. 2018

Annals of Applied Biology

100

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“…Meanwhile, field sprays of L. plantarum on Chinese cabbage alleviated the severity of soft rot by Pectobacterium carotovorum subsp. carotovorum 20 and contributed to a reduction of fire blight by Erwinia amylovora on apple and pear 21 . However, the aforementioned studies focused on the effects of L. plantarum on single organisms while its effects on the microbial community in a field remain to be investigated.…”

mentioning

confidence: 99%

Seasonal epiphytic microbial dynamics on grapevine leaves under biocontrol and copper fungicide treatments

Gobbi

Kyrkou

Filippi

et al. 2020

Sci Rep

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Winemakers have long used copper as a conventional fungicide treatment on grapevine and only recently, the use of biocontrol agents depicted a promising alternative. Most of the studies that investigate the impact of fungicide treatments, focus on specific pathogenic microbes. In the present work instead, a field experiment conducted in South Africa shows the seasonal microbial change occurring on grapevine leaves, periodically treated with two different fungicide treatments: copper sulphate and Lactobacillus plantarum MW-1. In this work, NGS data were combined with strain-specific and community qpcRs to reveal the shift of the microbial communities throughout the growing season and highlight the impact of fungicides on the microbiota. Only the family of Lactobacillaceae systematically changed between treatments, while the bacterial community remained relatively stable over time. MW-1 was exclusively detected on biocontrol-sprayed leaves. Conversely, the fungal community was largely shaped by sampling time, underlining the succession of different dominant taxa over the months. Between treatments, only a few fungal taxa appeared to change significantly and the fungal load was also comparable. Monitoring the dynamics of the microbial community under different fungicide treatments may advise the best timing to apply treatments to the plants, toward the realization of more sustainable agricultural practices.

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“…plantarum on Chinese cabbage alleviated the severity of soft rot by Pectobacterium carotovorum subsp. carotovorum (Tsuda et al 2016) and contributed to a reduction of fire blight by Erwinia amylovora on apple and pear (Daranas et al 2018). However, the aforementioned studies focused on the effects of L .…”

Section: Introductionmentioning

confidence: 99%

Seasonal microbial dynamics on grapevine leaves under biocontrol and copper fungicide treatments

Gobbi

Kyrkou

Filippi

et al. 2019

Preprint

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11Winemakers have long used copper as a fungicide on grapevine. However, the potential of copper to 12 accumulate on soil and affect the biota poses a challenge to achieving sustainable agriculture. One 13 recently developed option is the use of biocontrol agents to replace or complement traditional methods. 14 In the present study, a field experiment was conducted in South Africa in which the leaves in two blocks 15 of a vineyard were periodically treated with either copper sulphate or sprayed with Lactobacillus 16 plantarum MW-1 as a biocontrol agent. This study evaluated the impact of the two treatments on the 17 bacterial and fungal communities as they changed during the growing season. To do this, NGS was 18 combined with quantitative strain-specific and community qPCRs. The results revealed the progression 19 of the microbial communities throughout the season and how the different treatments affected the 20 microbiota. Bacteria appeared to be relatively stable at the different time points, with the only taxa that 21 systematically changed between treatments being Lactobacillaceae, which included reads from the 22 biocontrol agent. Cells of Lactobacillus plantarum MW-1 were only detected on treated leaves using 23 strain-specific qPCR, with its amount spanning from 10 3 to 10 5 cells/leaves. Conversely the fungal 24 community was largely shaped by a succession of different dominant taxa over the months. Between 25

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Biological control of bacterial soft rot in Chinese cabbage by Lactobacillus plantarum strain BY under field conditions

Cited by 53 publications

References 23 publications

Biological control of bacterial plant diseases with Lactobacillus plantarum strains selected for their broad‐spectrum activity

Biological control of bacterial plant diseases with Lactobacillus plantarum strains selected for their broad‐spectrum activity

Seasonal epiphytic microbial dynamics on grapevine leaves under biocontrol and copper fungicide treatments

Seasonal microbial dynamics on grapevine leaves under biocontrol and copper fungicide treatments

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