Biological Control of Bacterial Fruit Blotch of Watermelon Pathogen (Acidovorax citrulli) with Rhizosphere Associated Bacteria

Yadav, Dil Raj; Kim, Sang Woo; Um, Young Hyun; Kim, Hyun Seung; Lee, Seong Chan; Song, Jeong Young; Kim, Hong Gi; Lee, Youn Su

doi:10.5423/ppj.oa.09.2016.0187

Cited by 39 publications

(20 citation statements)

References 27 publications

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“…In our study, this family was the most abundant in the rhizosphere of both plants. With respect to the possible role in the plant tolerance to abiotic stress, several genus of this family have been described for their ability to control of plant pathogen [76], phosphate solubilization, phytohormone production, siderophores production [77,78].…”

Section: Discussionmentioning

confidence: 99%

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

et al. 2020

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The rhizosphere microbiome is key in survival, development, and stress tolerance in plants. Salinity, drought, and extreme temperatures are frequent events in the Atacama Desert, considered the driest in the world. However, little information of the rhizosphere microbiome and its possible contribution to the adaptation and tolerance of plants that inhabit the desert is available. We used a high-throughput Illumina MiSeq sequencing approach to explore the composition, diversity, and functions of fungal and bacterial communities of the rhizosphere of Baccharis scandens and Solanum chilense native plants from the Atacama Desert. Our results showed that the fungal phyla Ascomycota and Basidiomycota and the bacterial phyla Actinobacteria and Proteobacteria were the dominant taxa in the rhizosphere of both plants. The linear discriminant analysis (LDA) effect size (LefSe) of the rhizosphere communities associated with B. scandens showed the genera Penicillium and Arthrobacter were the preferential taxa, whereas the genera Oidiodendron and Nitrospirae was the preferential taxa in S. chilense. Both plant showed similar diversity, richness, and abundance according to Shannon index, observed OTUs, and evenness. Our results indicate that there are no significant differences (p = 0.1) between the fungal and bacterial communities of both plants, however through LefSe, we find taxa associated with each plant species and the PCoA shows a separation between the samples of each species. This study provides knowledge to relate the assembly of the microbiome to the adaptability to drought stress in desert plants.

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Section: Discussionmentioning

confidence: 99%

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

et al. 2020

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“…Therefore, the relationships between the corresponding secondary metabolites produced by NEAU-HV1 T and the antifungal activity are still ambiguous. To date, only 10 species of Sinomonas have been described, and S. atrocyaneus exhibited antimicrobial activity against Acidovorax citrulli , which is the pathogen of bacterial fruit blotch [12]. To the best of our knowledge, this is the first report on the antifungal activity of Sinomonas against plant pathogenic fungi.…”

Section: Resultsmentioning

confidence: 99%

“…Some members of the genus Sinomonas have been reported to be able to synthesize silver nanoparticles with antimicrobial activity [8], hydrolyze starch [9], biodesulfurize coal [10], and degrade sesamin [11]. Recently, S. atrocyaneus has exhibited plant growth promoting effects and antagonistic activity against a broad-spectrum of root and foliar pathogens, implying its potential use in sustainable agriculture [12]. In this study, a strain NEAU-HV1 T with inhibitory activity against phytopathogenic fungi Exserohilum turcicum was isolated from a soil sample.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Sinomonas gamaensis sp. nov., a Novel Soil Bacterium with Antifungal Activity against Exserohilum turcicum

Yan

Liu

et al. 2019

Microorganisms

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A novel Gram staining positive, aerobic bacterium NEAU-HV1T that exhibits antifungal activity against Exserohilum turcicum was isolated from a soil collected from Gama, Hadjer lamis, Chad. It was grown at 10–45 °C (optimum 30 °C), pH 5–10 (optimum pH 8), and 0–4% (w/v) NaCl (optimum 1%). Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-HV1T was closely related to Sinomonas susongensis A31T (99.24% sequence similarity), Sinomonas humi MUSC 117T (98.76%), and Sinomonas albida LC13T (98.68%). The average nucleotide identity values between NEAU-HV1T and its most closely related species were 79.34−85.49%. The digital DNA–DNA hybridization values between NEAU-HV1T and S. susongensis A31T, S. albida LC13T, and S. humi MUSC 117T were 23.20, 23.50, and 22.80%, respectively, again indicating that they belonged to different taxa. The genomic DNA G+C content was 67.64 mol%. The whole cell sugars contained galactose, mannose, and rhamnose. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, and four glycolipids. The respiratory quinone system comprised MK-9(H2), MK-10(H2), and MK-8(H2). The major cellular fatty acids (>5%) were anteiso-C15:0, anteiso-C17:0, C16:0, and iso-C15:0. Based on the polyphasic analysis, it is suggested that the strain NEAU-HV1T represents a novel species of the genus Sinomonas, for which the name Sinomonas gamaensis sp. nov. is proposed. The type strain is NEAU-HV1T (= DSM 104514T = CCTCC M 2017246T).

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“…Based on this differential virulence, DNA finger-printing and biochemical properties have characterized two major groups of A. citrulli , with group II strains being predominantly isolated from watermelon hosts, while group I strains are associated with non-watermelon hosts [35]. Each strain shows aggressive virulence to its corresponding hosts [45,65]. This defined group II strains are more virulent towards watermelon.…”

Section: Discussionmentioning

confidence: 99%

Development of Molecular Markers for Detection of Acidovorax citrulli Strains Causing Bacterial Fruit Blotch Disease in Melon

Islam¹,

Hossain²,

Kim³

et al. 2019

IJMS

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Acidovorax citrulli (A. citrulli) strains cause bacterial fruit blotch (BFB) in cucurbit crops and affect melon significantly. Numerous strains of the bacterium have been isolated from melon hosts globally. Strains that are aggressively virulent towards melon and diagnostic markers for detecting such strains are yet to be identified. Using a cross-inoculation assay, we demonstrated that two Korean strains of A. citrulli, NIHHS15-280 and KACC18782, are highly virulent towards melon but avirulent/mildly virulent to the other cucurbit crops. The whole genomes of three A. citrulli strains isolated from melon and three from watermelon were aligned, allowing the design of three primer sets (AcM13, AcM380, and AcM797) that are specific to melon host strains, from three pathogenesis-related genes. These primers successfully detected the target strain NIHHS15-280 in polymerase chain reaction (PCR) assays from a very low concentration of bacterial gDNA. They were also effective in detecting the target strains from artificially infected leaf, fruit, and seed washing suspensions, without requiring the extraction of bacterial DNA. This is the first report of PCR-based markers that offer reliable, sensitive, and rapid detection of strains of A. citrulli causing BFB in melon. These markers may also be useful in early disease detection in the field samples, in seed health tests, and for international quarantine purposes.

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Biological Control of Bacterial Fruit Blotch of Watermelon Pathogen (Acidovorax citrulli) with Rhizosphere Associated Bacteria

Cited by 39 publications

References 27 publications

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

Fungal and Bacterial Microbiome Associated with the Rhizosphere of Native Plants from the Atacama Desert

Characterization of Sinomonas gamaensis sp. nov., a Novel Soil Bacterium with Antifungal Activity against Exserohilum turcicum

Development of Molecular Markers for Detection of Acidovorax citrulli Strains Causing Bacterial Fruit Blotch Disease in Melon

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