Biological Compatibility Profile on Biomaterials for Bone Regeneration

Kampleitner, Carina; Obi, Jessika; Vassilev, Nicola; Epstein, Michelle M.; Hoffmann, Oskar

doi:10.3791/58077

Cited by 4 publications

(3 citation statements)

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“…To evaluate biocompatibility of novel and potent biomaterials, most often, studies have been applied in animal, in vitro and in vivo, models [ 75 , 76 , 77 ]. In addition, when evaluating rodent or human immune cellular reactions to biomaterials, focus has often been on early innate responses [ 57 , 78 ], such as that recently thoroughly studied by Krüger-Genge et al by their combined approach to test the immunocompatibility of gelatin-based hydrogels, of interest for production of vascular grafts [ 79 ].…”

Section: Discussionmentioning

confidence: 99%

In Vitro Study of Human Immune Responses to Hyaluronic Acid Hydrogels, Recombinant Spidroins and Human Neural Progenitor Cells of Relevance to Spinal Cord Injury Repair

Cai

Ekblad‐Nordberg

Michaëlsson

et al. 2021

Cells

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Scaffolds of recombinant spider silk protein (spidroin) and hyaluronic acid (HA) hydrogel hold promise in combination with cell therapy for spinal cord injury. However, little is known concerning the human immune response to these biomaterials and grafted human neural stem/progenitor cells (hNPCs). Here, we analyzed short- and long-term in vitro activation of immune cells in human peripheral blood mononuclear cells (hPBMCs) cultured with/without recombinant spidroins, HA hydrogels, and/or allogeneic hNPCs to assess potential host–donor interactions. Viability, proliferation and phenotype of hPBMCs were analyzed using NucleoCounter and flow cytometry. hPBMC viability was confirmed after exposure to the different biomaterials. Short-term (15 h) co-cultures of hPBMCs with spidroins, but not with HA hydrogel, resulted in a significant increase in the proportion of activated CD69+ CD4+ T cells, CD8+ T cells, B cells and NK cells, which likely was caused by residual endotoxins from the Escherichia coli expression system. The observed spidroin-induced hPBMC activation was not altered by hNPCs. It is resource-effective to evaluate human compatibility of novel biomaterials early in development of the production process to, when necessary, make alterations to minimize rejection risk. Here, we present a method to evaluate biomaterials and hPBMC compatibility in conjunction with allogeneic human cells.

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Section: Discussionmentioning

confidence: 99%

In Vitro Study of Human Immune Responses to Hyaluronic Acid Hydrogels, Recombinant Spidroins and Human Neural Progenitor Cells of Relevance to Spinal Cord Injury Repair

Cai

Ekblad‐Nordberg

Michaëlsson

et al. 2021

Cells

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“…To study the effect of the methanolic extract, microfractions and compounds on osteoclastogenesis, we used a primary mouse co-culture model of calvarial-derived neonatal OB, isolated by enzymatic digestion, and bone-marrow OC precursors as previously described [ 41 , 42 ]. OB (4.2–6.5 × 10 4 cells per cm 2 ) were seeded on tissue culture plastic in a culture medium containing ⍺MEM (Gibco, Thermo Fisher Scientific, Vienna, Austria), 10% heat-inactivated fetal bovine serum (FBS, Gibco) and 1% penicillin/streptomycin (Gibco).…”

Section: Methodsmentioning

confidence: 99%

“…For TRAP staining, we followed a protocol that was previously reported in Kampleitner et al [ 41 ]. Briefly, cells were fixed in 10% buffered formalin at room temperature on day 5 of co-culture and incubated with the TRAP staining solution at 37 °C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Biochemometry-Based Discovery of Phenylpropanoids from Azadirachta indica Fruits as Inhibitors of In Vitro Osteoclast Formation

Tahir

Kampleitner²,

Wirglauer³

et al. 2022

Molecules

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(1) Background: Inhibition of osteoclast differentiation is the key approach in treating osteoporosis. However, using state-of-the-art treatments such as bisphosphonates and estrogen-based therapy is usually accompanied by many side effects. As opposed to this, the use of natural products as an osteoporotic remedy delivers promising outcomes with minimal side effects. (2) Methods: In the present study, we implemented a biochemometric workflow comprising (i) chemometric approaches using NMR and mass spectrometry and (ii) cell biological approaches using an osteoclast cytochemical marker (TRAP). The workflow serves as a screening tool to pursue potential in vitro osteoclast inhibitors. (3) Results: The workflow allowed for the selective isolation of two phenylpropanoids (coniferyl alcohol and sinapyl alcohol) from the fruits of neem tree (Azadirachta indica). These two isolated phenylpropanoids showed a very promising dose-dependent inhibition of osteoclast differentiation with negligible effects in terms of cell viability. (4) Conclusion: The presented workflow is an effective tool in the discovery of potential candidates for osteoclast inhibition from complex extracts. The used biochemometric approach saves time, effort and costs while delivering precise hints to selectively isolate bioactive constituents.

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Phase composition of calcium phosphate materials affects bone formation by modulating osteoclastogenesis

Humbert,

Kampleitner,

De Lima

et al. 2024

Acta Biomaterialia

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Biological Compatibility Profile on Biomaterials for Bone Regeneration

Cited by 4 publications

References 0 publications

In Vitro Study of Human Immune Responses to Hyaluronic Acid Hydrogels, Recombinant Spidroins and Human Neural Progenitor Cells of Relevance to Spinal Cord Injury Repair

In Vitro Study of Human Immune Responses to Hyaluronic Acid Hydrogels, Recombinant Spidroins and Human Neural Progenitor Cells of Relevance to Spinal Cord Injury Repair

Biochemometry-Based Discovery of Phenylpropanoids from Azadirachta indica Fruits as Inhibitors of In Vitro Osteoclast Formation

Phase composition of calcium phosphate materials affects bone formation by modulating osteoclastogenesis

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