Biological characterization and phylogenetic analysis of a novel genetic group of Newcastle disease virus isolated from outbreaks in commercial poultry and from backyard poultry flocks in Pakistan

Munir, Muhammad; Cortey, Martı́; Abbas, Muhammad; Qureshi, Zafar ul Ahsan; Afzal, Farhan; Shabbir, Muhammad Zubair; Khan, Muhammad Tanveer; Ahmed, Safia; Ahmad, Saeed; Baule, Claudia; Ståhl, Karl; Zohari, Siamak; Berg, Mikael

doi:10.1016/j.meegid.2012.02.015

Cited by 75 publications

(76 citation statements)

References 36 publications

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“…Phylogenetic analysis revealed that these isolates were located in VIIb subgenotype. In Pakistan (the neighboring country of Iran), Munir et al [9] reported that all Pakistani NDV isolates were considered velogenic with the motif RRQKRF. This analysis clearly demonstrated that PAK strains and former lineage 7 are closely related to lineage 5, and these are not distinct enough to be regarded as new lineages.…”

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confidence: 99%

Molecular characterization and phylogenetic study of the fusion genes of Newcastle disease virus from the recent outbreaks in Ahvaz, Iran

2016

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Newcastle disease (ND) is an acute and highly contagious disease affecting many domestic and wild species of birds. Its effects are most notable in domestic poultry due to their high susceptibility and the potential for severe impacts of an epizootic on the poultry industry. In this study, partial sequences of fusion genes of three Newcastle disease virus (NDV) isolates collected during 2013-14 outbreaks from the vaccinated commercial broiler chicken farms with high mortality around Ahvaz city (Southwest of Iran) were characterized. All three isolates showed the amino acid sequence 112RRQKRF117 at the C-terminus of the F2 protein and phenylalanine at the N-terminus of the F1 protein residue 117. These amino acid sequences were identical to a known virulent motif. The phylogenetic analysis revealed that the Iranian ND isolates in this study are closely related to the genotype VIId of class II NDV strains. Our results specified that there are velogenic NDV circulating in Ahvaz commercial broiler flocks and causing outbreaks in poultry industry.

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confidence: 99%

Molecular characterization and phylogenetic study of the fusion genes of Newcastle disease virus from the recent outbreaks in Ahvaz, Iran

2016

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“…Biological characterization and phylogenetic analysis the fusion (F), hemagglutininneuraminidase (HN) and matrix (M) genes of Newcastle disease virus isolated from outbreaks in commercial poultry and from backyard poultry flocks in Pakistan based indicated the emergence of a novel genetic group within lineage 5, distinct from isolates previously reported in the region [15]. Munir et al (2012) in study on velogenic Newcastle disease virus isolated from a healthy backyard poultry flock in 2010, showed an Y526Q substitution in the HN protein, which determines neuraminidase receptor binding and fusion activity of NDV [15]. Two NDV isolated from Iranian farms are clustered in one group in genotype VIb/2, which from high homology (95.34%) with Rus/Moscow/0453/09(VIb/2) (JF824064) and Pi/Rus/Vladimir/495/04(VIb/2) (JF824029).…”

Section: Discussionmentioning

confidence: 97%

Molecular Characterization and Phylogenetic Study of Newcastle Disease Viruses Isolated in Iran, 2014–2015

et al. 2016

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Newcastle disease (ND) is a highly contagious disease that affects many species of birds and causes significant economic losses to the poultry industry worldwide and the pathogenicity of Newcastle disease virus (NDV) strains varies with different virulence. Samples were collected from chicken commercial farms in Iran during 2014-2015. ND virus was characterized (NDV) by partial sequences of fusion genome and compared with other NDV sequences. All viruses showed the amino acid sequence 112 RRQKRF117 at the C-terminus of the F2 protein and phenylalanine at the N-terminus of the F1 protein, residue 117. These amino acid sequences were identical to a known virulent motif. Based on the phylogenetic analysis the Iranian NDV isolates are closely related to the genotype VIId of class II NDV strains. The result showed that the genotype VIId was dominant and circulating among chicken farms and caused clinical disease.

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“…Blood was stored on QIAcard FTA Indicator Four Spots (Qiagen, Hilden, Germany), and shipped at ambient temperature from Pakistan to the Swedish University of Agricultural Sciences (SLU), Uppsala, Sweden, for processing. These QIAcards have the ability to inactivate the samples and preserve nucleic acids, which is proven to be an efficient and economical means of sample transportation [24][25][26]. In parallel, tissue samples were also collected and transported to Veterinary Research Institute (VRI), Lahore, Pakistan.…”

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confidence: 99%

Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates

et al. 2013

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Several outbreaks of avian influenza (AI) caused by H9N2 subtype, have been reported in the poultry industry during 1990 around the globe. Currently, H9N2 are endemic in the large area of Middle and Far East, including Pakistan. Since H9N2 AI viruses are sporadically reported from humans, extensive incidence of H9N2 in poultry imposes a great risk for human health. In this context, continuous monitoring of the poultry and determining the genetic nature of these viruses are fundamental to predict any future threat. Thus gene sequences of one isolate of H9N2, isolated from commercial poultry flocks, were analyzed. The results of this investigation, based on hemagglutinin (HA), neuraminidase (NA) and nonstructural genes, showed that Pakistani H9N2 isolates are closely related to each other and to other H9N2 isolates from the Middle East. However, several unusual substitutions with unknown functional consequences were observed in HA and NA proteins and thus warrant further investigations for their possible role in viral biology. In conclusion, these findings provide information regarding the genetic nature of H9N2 avian influenza viruses in Pakistani poultry and necessitate the sequencing of more H9N2 viruses from both naturally infected and vaccinated flocks.

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Biological characterization and phylogenetic analysis of a novel genetic group of Newcastle disease virus isolated from outbreaks in commercial poultry and from backyard poultry flocks in Pakistan

Cited by 75 publications

References 36 publications

Molecular characterization and phylogenetic study of the fusion genes of Newcastle disease virus from the recent outbreaks in Ahvaz, Iran

Molecular characterization and phylogenetic study of the fusion genes of Newcastle disease virus from the recent outbreaks in Ahvaz, Iran

Molecular Characterization and Phylogenetic Study of Newcastle Disease Viruses Isolated in Iran, 2014–2015

Isolation and characterization of low pathogenic H9N2 avian influenza A viruses from a healthy flock and its comparison to other H9N2 isolates

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