Biological characteristics of cervical precancerous cell proliferation

Abstract

<jats:title>Abstract</jats:title><jats:p>High-grade squamous intraepithelial lesions (HSILs) are regarded as precancerous lesions that can progress to cervical carcinoma; however, it is very difficult to effectively differentiate these precancerous cells from cancerous cells based on morphology alone. Additionally, the difference between precancerous cells and cancerous cells in regard to biological behaviour remains unclear. We previously cultured primary normal uterine cervical keratinocytes from human normal cervical tissue and cervical precancerous cells that were naturally infected with human papillomavirus from small-sized neoplastic cervical tissues. Here, we extended our study to further observe the in vitro proliferative characteristics of cervical precancerous cells at the cellular and molecular levels. In this study, we found that the growth rate of precancerous cells was significantly faster than that of normal cervical cells and slower than that of Caski cells. However, the proliferative capacity of such precancerous cells was similar to that of cancerous cells of the cervix at the molecular level. These results suggest that the surrounding environment of the cells may play an important role in the development of cervical cancer, which provides an important basis for the further study of precancerous and cancerous lesions of the cervix.</jats:p>

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“…It was found that precancerous cells and CaSki cells have similar growth rate, higher than that of normal uterine cervix cells and higher percentage of Mediators of Inflammation aneuploid cells than normal cells. However, aneuploid and quadraploid cells constituted for about 25% of cells in normal cervical tissue vs. 35% and 37% in precancerous and CaSki cell lines, respectively [73]. The answer may lie in the ability of normal squamous epithelial cells to divide and constantly replace dead, superficial layer cells.…”