Biological characteristics of adipose tissue‐derived stem cells labeled with amine‐surface‐modified superparamagnetic iron oxide nanoparticles

Abstract

Cell labeling and tracking are becoming increasingly important areas within the field of stem cell transplantation. The ability to track the migration and distribution of implanted cells is critical to understanding the beneficial effects and mechanisms of stem cell therapy. The present study investigated the effects of amine-surface-modified superparamagnetic iron oxide (SPIO) nanoparticles on the biological properties of human adipose tissue-derived stem cells (hADSCs). Monodisperse hydrophobic magnetite (Fe3 O4 ) nanoparticles were prepared using silicon and surface-modified with amine coating. Cell viability, proliferation, differentiation potential, and surface marker expression were evaluated. The magnetic particles (10-18 nm) displayed high labeling efficiency and stability in hADSCs. SPIO-labeled cells produced a hypointense signal and were effectively visualized by MRI for up to 21 days. The results of MTT proliferation assays and flow cytometry analysis demonstrated that SPIOs were biocompatible, viz. the labeling process did not cause cell death or apoptosis and had no side effects on cell proliferation. In vivo experiments showed that the magnetic particles did not affect liver and kidney function. The successful and stable labeling of hADSCs combined with efficient magnetic tropism demonstrates that SPIOs are promising candidates for hADSC tracking in hADSC-based cell therapy applications.

Toxicity and Functional Impairment in Human Adipose Tissue-Derived Stromal Cells (hASCs) Following Long-Term Exposure to Very Small Iron Oxide Particles (VSOPs)

“…These results indicate that VSOPs-labelled hASCs can be expanded without affecting their viability and their ability to differentiate. This is consistent with the findings of others, who have not described any influence of IONPs on the differentiation capacity of different cell types, such as bone marrow-derived stem cells (BMSCs) [51,52], haNCSs [3] or ASCs [53].…”

Biological Characteristics of Fluorescent Superparamagnetic Iron Oxide Labeled Human Dental Pulp Stem Cells

“…Meanwhile, we also found that the fluorescence intensity ascended with the increasing of concentration of MIRB, which was also indicated by intracellular iron content analysis. However, the intracellular iron content in 100  μ g/mL group did not reach 2-fold higher than that of 50  μ g/mL group, possibly due to limited cell uptake because of Fe saturation or Fe particle clumping at high MIRB concentration [8]. …”

“…Odonto-/osteogenic differentiation was measured to assess the effect of MIRB on the transdifferentiation potential of hDPSCs as previously described by Wang et al [8] using differentiation medium (Cyagen Biosciences Inc., Santa Clara, CA, USA). For osteogenic differentiation, hDPSCs were seeded at 3.1 × 10 3 cells/cm 2 and labeled by different concentrations of MIRB (12.5  μ g/mL, 25  μ g/mL, and 50  μ g/mL) and then allowed to reach 90% confluence.…”

“…To distinguish specific cells using MRI, transplanted cells must be labeled with a magnetic contrast agent. In recent years, various magnetic nanoparticles, such as superparamagnetic iron oxide (SPIO), have been widely applied both in cell tracking and in magnetic targeting [8–10]. However, before being applied for both experimental animals and eventually clinical use, the biological impacts of different kinds of SPIO on different types of stem cells must be investigated.…”

Potential role of tracing stem cell transplantation and effects on the immune cell function of ferumoxytol combining with heparin and protamine in vivo/in vitro

“…The induction methods have been reported previously by our group (Wang et al, 2015). The induction methods have been reported previously by our group (Wang et al, 2015).…”