Biological Characteristics and Biomedical Applications of the Squid <i>Sepioteuthis lessoniana</i> Cultured Through Multiple Generations

Lee, P G; Turk, Philip E.; Yang, Won Tack; Hanlon, Roger T.

doi:10.2307/1542279

Cited by 91 publications

(64 citation statements)

References 47 publications

(21 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…By using standard histological techniques, it describes the development of the CNS and peripheral nerves of the oval squid Sepioteuthis lessoniana during its development and paralarval stages. This squid has served already as a suitable species in several biological studies and in laboratory tests (Lee et al, 1994), and the timetable of its CNS development given in this study may serve in the future as a reference for similar embryologic studies in other squid (or cephalopod) species.…”

Section: Indexing Terms: Neuropil; Brain Formation; Giant Axon; Invermentioning

confidence: 94%

Embryonic and paralarval development of the central nervous system of the loliginid squid Sepioteuthis lessoniana

Shigeno

Tsuchiya

Segawa

2001

J of Comparative Neurology

View full text Add to dashboard Cite

The embryonic development of the central nervous system (CNS) in the oval squid Sepioteuthis lessoniana is described. It has three distinct phases: (1) The ganglionic accumulation phase: Ganglionic cell clusters develop by ingression, migration, and accumulation of neuroblasts. (2) The lobe differentiation phase: Ganglia differentiate into lobes. The phase is identified by the beginning of an axogenesis. During this phase, neuropils are first formed in the suboesophageal mass, then in the basal lobe system, and finally in the inferior frontal lobes and the superior frontal-vertical lobe systems. (3) The neuropil increment phase: After the shape of the lobes reached its typical form, neuropil growth occurs, specifically in the vertical lobe. The paralarval central nervous system (CNS) is characterized by neuronal gigantism of the giant fibers and some suboesophageal commissures and connectives. The neuropil formation in the CNS of S. lessoniana occurs somewhat earlier than in Octopus vulgaris, although the principal developmental plan is quite conservative among the other coleoids investigated. Some phylogenetic aspects are discussed based on the similarities in the morphologic organization of their brains.

show abstract

Section: Indexing Terms: Neuropil; Brain Formation; Giant Axon; Invermentioning

confidence: 94%

Embryonic and paralarval development of the central nervous system of the loliginid squid Sepioteuthis lessoniana

Shigeno

Tsuchiya

Segawa

2001

J of Comparative Neurology

View full text Add to dashboard Cite

show abstract

“…Wild embryos collected between 1999 and 2004 from several near-shore locations (Gulf of Thailand; Okinawa Island, Japan; Izo, Japan) were reared as described in Lee et al (1994).…”

Section: Methodsmentioning

confidence: 99%

Ontogeny of mantle musculature and implications for jet locomotion in oval squidSepioteuthis lessoniana

Thompson

Kier

2006

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARY We examined the relationship between mantle muscle structure and mantle kinematics in an ontogenetic series (5-85 mm dorsal mantle length) of oval squid, Sepioteuthis lessoniana. Thick filament length increased during growth in the mantle muscle fibres that power jet locomotion (i.e. the circular muscles). The thick filament length of both the superficial mitochondria-rich (SMR; analogous to vertebrate red muscle fibres) and central mitochondria-poor (CMP; analogous to vertebrate white muscle fibres) circular muscles increased significantly during ontogeny. Thick filaments in the SMR circular muscle fibres of newly hatched squid (N=5) ranged from 0.7 to 1.4 μm and averaged 1.0 μm, while the thick filaments of the SMR fibres of the largest squids (N=4) studied ranged from 1.2 to 3.4μm and averaged 1.9 μm. The ontogeny of thick filament length in the CMP circular muscle fibres showed a similar trend. The range for hatchling CMP circular muscles was 0.7-1.4 μm, with an average of 1.0 μm, whereas the range and average for the largest squids studied were 0.9-2.2 μm and 1.5μm, respectively. Within an individual hatchling, we noted no significant differences between the thick filament lengths of the SMR and CMP fibres. Within an individual juvenile, the thick filaments of the SMR fibres were∼25% longer than the CMP fibres. The change in thick filament length may alter the contractile properties of the circular muscles and may also result in a decrease in the rate of mantle contraction during jetting. In escape-jet locomotion, the maximum rate of mantle contraction was highest in newly hatched squid and declined during ontogeny. The maximum rate of mantle contraction varied from 7-13 muscle lengths per second in newly hatched squid(N=14) and from 3-5 muscle lengths per second in the largest squids(N=35) studied.

show abstract

“…During the prey strike, the eight arms flare open and the two tentacles are rapidly elongated so that the terminal portion of the tentacles, which is equipped with suckers, contacts and attaches to the prey (Chen et al, 1996;Fields, 1965;Hurley, 1976;Kier, 1982;Kier and van Leeuwen, 1997;LaRoe, 1971;Lee et al, 1994;Neill and Cullen, 1974;Nicol and O'Dor, 1985). Kinematic analysis of high-speed cine films of the strike shows that the elongation occurs in only 20-40·ms, the peak strain in the tentacle stalks ranges from 0.43 to 0.8, the peak longitudinal strain rates range from 23 to 45·s -1 , the peak velocity is greater than 2·m·s -1 and the peak acceleration is approximately 250·m·s -2 .…”

Section: Squid Prey Capturementioning

confidence: 99%

Muscle specialization in the squid motor system

Kier¹

2006

Journal of Biomechanics

View full text Add to dashboard Cite

SummaryAlthough muscle specialization has been studied extensively in vertebrates, less is known about the mechanisms that have evolved in invertebrate muscle that modulate muscle performance. Recent research on the musculature of squid suggests that the mechanisms of muscle specialization in cephalopods may differ from those documented in vertebrates. Muscle diversity in the development and the evolution of cephalopods appears to be characterized by modulation of the dimensions of the myofilaments, in contrast to the relatively fixed myofilament dimensions of vertebrate muscle. In addition, the arrangement of the myofilaments may also be altered, as has been observed in the extensor muscle fibres of the prey capture tentacles of squid and cuttlefish, which show cross-striation and thus differ from the obliquely striated pattern of most cephalopod locomotor muscle fibres. Although biochemical specializations that reflect differences in aerobic capacity have been documented previously for specific layers of the mantle muscle of squid, comparison of protein profiles of myofilament preparations from the fast crossstriated tentacle fibres and slow obliquely striated fibres from the arms has revealed remarkably few differences in myofilament lattice proteins. In particular, previous studies using a variety of SDS-PAGE techniques and peptide mapping of the myosin heavy chain were unable to resolve differences in the myosin light and heavy chains. Since these techniques cannot exclude the presence of a highly conserved variant that differs in only a few amino acids, in this study semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) analysis of myosin heavy chain messenger RNAs (mRNAs) from the cross-striated tentacle and obliquely striated arm muscle fibres was conducted. This analysis showed that a previously reported alternatively spliced isoform of the squid myosin motor domain is present only in low abundance in both muscle types and therefore differential expression of the two myosins cannot explain the difference in contractile properties. It thus appears that modulation of the contractile properties of the musculature of squid and other cephalopods occurs primarily through variation in the arrangement and dimensions of the myofilaments.

show abstract

Biological Characteristics and Biomedical Applications of the Squid Sepioteuthis lessoniana Cultured Through Multiple Generations

Cited by 91 publications

References 47 publications

Embryonic and paralarval development of the central nervous system of the loliginid squid Sepioteuthis lessoniana

Embryonic and paralarval development of the central nervous system of the loliginid squid Sepioteuthis lessoniana

Ontogeny of mantle musculature and implications for jet locomotion in oval squidSepioteuthis lessoniana

Muscle specialization in the squid motor system

Contact Info

Product

Resources

About