Biological Characteristic of Pathogenic Fungus Causing Anthracnose of Loquat Fruit

Liu, A.Y.; Chen, W.X.; Gu, Hongru; Shi, Jie; Li, J.

doi:10.17660/actahortic.2007.750.74

Cited by 10 publications

(9 citation statements)

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“…In contrast, the temperature of the conidial suspensions was not higher than 40 °C, and unlikely to have effected germination. The lethal temperature for closely related Colletotrichum acutatum spores is 50 °C with a required exposure time of 5 min (Liu et al , ), much higher than the temperature of the conidial suspensions in this study. The successful reduction of germination following CP treatment of conidial suspensions suggests that CP may be a useful alternative method for treating wash water in packhouses compared to chemical treatments, and this now requires further study.…”

Section: Discussionmentioning

confidence: 67%

Cold plasma as a novel treatment to reduce the in vitro growth and germination of Colletotrichum species

2019

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This study investigated the use of cold plasma to reduce the in vitro growth of two postharvest fungal plant pathogens, Colletotrichum alienum and C. fioriniae, isolated from avocados. Cold plasma (CP) was used to treat pure cultures and conidial suspensions of both pathogens, for 180 or 360 s, in either open or sealed environments from varying distances. In an open environment, the 360 s treatment at a distance of 5 cm reduced the colony growth of freshly inoculated cultures to less than 2 mm/day compared to the control of more than 8 mm/day, and treatment of conidial suspensions resulted in almost 100% reduction of conidial germination. In the same environment, the 180 s CP treatment did not significantly reduce the colony growth of fresh or actively growing cultures, but did suppress the germination of conidia by up to 80%. In a sealed environment, the 360 s CP treatment also effectively reduced the growth of freshly inoculated cultures, with no growth for some isolates. Production of reactive oxygen and nitrogen species was observed during treatment, and these may have contributed to the reduction in growth and germination. These results demonstrate the potential of CP for the control of two Colletotrichum species.

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Section: Discussionmentioning

confidence: 67%

Cold plasma as a novel treatment to reduce the in vitro growth and germination of Colletotrichum species

2019

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“…Loquat fruit has a very short shelf life and sensitive to loss of nutrients, moisture, mechanical and physical damage, and microbial decay [ 6 ]. Several fungal pathogens, including Alternaria alternata , Aspergillus niger , Botrytis cinerea , Colletotrichum gloeosporioides , Curlaria lunata , Diplocarpon mespili , Diplodia natalensis , Diplodia seriata , Geotrichum candidum , Fusarium solani , Mucor fragilis , Pestalotia sp., Phytophthora palmivora and Spilocaea pyracanthae infest loquat [ 7 – 19 ].…”

Section: Introductionmentioning

confidence: 99%

Diversity of fungal pathogens associated with loquat and development of novel virulence scales

et al. 2021

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Loquat [Eriobotrya japonica (Thunb.) Lindl.] is an important fruit crop in Pakistan; however, a constant decline in its production is noted due biotic and abiotic stresses, particularly disease infestation. Fungal pathogens are the major disease-causing agents; therefore, their identification is necessary for devising management options. This study explored Taxila, Wah-Cantt, Tret, Chatar, Murree, Kalar-Kahar, Choa-Saidan-Shah and Khan-Pur districts in the Punjab and Khyber Paktoon Khawa (KPK) provinces of Pakistan to explore the diversity of fungal pathogens associated with loquat. The samples were collected from these districts and their microscopic characterizations were accomplished for reliable identification. Alternaria alternata, Curvularia lunata, Lasiodiplodia theobromae, Aspergilus flavis, Botrytis cinerea, Chaetomium globosum, Pestalotiopsis mangiferae and Phomopsis sp. were the fungal pathogens infesting loquat in the study area. The isolates of A. alternata and C. lunata were isolated from leaf spots and fruit rot, while the isolates of L. theobromae were associated with twig dieback. The remaining pathogens were allied with fruit rot. The nucleotide evidence of internal transcribed spacer (ITS) regions (ITS1, 5.8S, and ITS2) were computed from all the pathogens and submitted in the database of National Center for Biotechnology Information (NCBI). For multigene analysis, beta-tubulin (BT) gene and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) regions were explored for A. alternata and C. lunata isolates, respectively. The virulence scales of leaf spots, fruit rot, and twig dieback diseases of loquat were developed for the first time through this study. It is the first comprehensive study with morpho-molecular identification, and newly developed virulence scales of the fungal pathogens associated with loquat, which improves the understanding of these destructive diseases.

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“…According to previous reports, the pH for growth of different Colletotrichum spp. varies from 3 to 10, with optimum germination in vitro at pH 5 ± 1 (Liu et al, 2007;Sun et al, 2008;Kenny et al, 2012). Although the pH decreased during PAW generation in the present investigation, it did not appear to be the actual mechanism for reducing conidial germination.…”

Section: Discussionmentioning

confidence: 99%

Plasma‐activated water inhibits in vitro conidial germination of Colletotrichum alienum, a postharvest pathogen of avocado

2020

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Cold plasma, an ionized gas produced by applying an electrical current to air, can be used to produce plasma‐activated water (PAW), which has excellent antimicrobial properties. In this study PAW was applied to conidia of Colletotrichum alienum to investigate its impact on conidial germination in vitro. PAW was produced by treating tap, deionized, or distilled water with cold plasma for 30 or 60 min to produce PAW30 or PAW60, each of which was then incubated for up to 24 hr with a conidial suspension of C. alienum in a ratio of 1:1, 1:2, or 1:3 (conidial suspension:PAW), and the percentage germination measured. The greatest reduction in germination occurred when conidia were incubated with PAW60 produced from deionized water or distilled water, for all ratios. For PAW30, deionized water was the most effective for all three ratios, and on this basis, deionized water was selected for all further experiments. PAW produced from smaller volumes of water and at shorter distances from the cold plasma source was more effective at reducing germination. Treatment of conidia with acidified water was not as effective as PAW at inhibiting germination. Nitrates and nitrites were present in the PAW in varying concentrations and may have contributed to the inhibition of germination. PAW retained activity and reduced germination even after storage for 15 days. These findings demonstrate the potential of PAW as a novel treatment for postharvest fungal pathogens.

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Biological Characteristic of Pathogenic Fungus Causing Anthracnose of Loquat Fruit

Cited by 10 publications

References 0 publications

Cold plasma as a novel treatment to reduce the in vitro growth and germination of Colletotrichum species

Cold plasma as a novel treatment to reduce the in vitro growth and germination of Colletotrichum species

Diversity of fungal pathogens associated with loquat and development of novel virulence scales

Plasma‐activated water inhibits in vitro conidial germination of Colletotrichum alienum, a postharvest pathogen of avocado

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