Biological characterisation of Australian isolates of chicken anaemia agent

Connor, Thomas J.; McNeilly, F.; Firth, G. A.; McNulty, M. S.

doi:10.1111/j.1751-0813.1991.tb03192.x

Cited by 15 publications

(9 citation statements)

References 20 publications

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“…The present findings and the findings of Todd et al (1992) support the view that CAV isolates can be differentiated with number of restriction site differences occurring between isolates, though these isolates are antigenically and pathologically indistinguishable (McNulty et al, 1990;Connor et al, 1991;Yuasa and Imai, 1986). However, the findings of Todd et al (1992) were confined with only 675 bp fragment of the CAV genome that is equivalent to fragment C of the present study.…”

Section: Discussionsupporting

confidence: 82%

Restriction endonuclease analysis of the genomes of different isolates of chicken anemia virus amplified by polymerase chain reaction

et al. 2018

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Four DNA fragments (fragments A, B, C and D) covering the whole genome of chicken anemia virus (CAV) were amplified enzymatically by polymerase chain reaction (PCR) using four pairs of oligonucleotide primers. The DNA fragments were amplified from each of nine CAV isolates including eight Malaysian isolates and one European isolate (Cux-1). For all nine CAV isolates, fragment A (1518 bp) was digested with one restriction enzyme, Eco130I (StyI); fragment B (926 bp) with three enzymes, Eco130I (StyI), HpaII and MboI separately; fragment C (675 bp) with also three enzymes, BsuRI (HaeIII), HinfI, and HpaII separately; and the fragment D (552 bp) with one enzyme, EcoRI. Enzyme digested products of different fragments were separated by agarose gel or polyacrylamide gel electrophoresis. Each of the eightenzymatic reactions differentiated at least two isolates except the HpaII digestion of fragment C where no isolate was distinguished. The overall restriction endonuclease (RE) analysis separated four isolates (BL-1, BL-2, BL-4 and BL-5) in one group and the rest five isolates (SMSC-1, SMSC-2, 3-1, BL-3 and Cux-1) were differentiated from each other and also from the group of four isolates, based on the number of restriction site differences and the fragments generated by different enzymatic digestions. The study revealed that RE analysis could be used to identify and differentiate CAV isolates based on the number of restriction site differences. The study showed that more isolates, even the isolates from the same poultry farm, could be differentiated with proper genomic diversity after RE analysis of more genome fragments compared to that of single genome fragment.

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Section: Discussionsupporting

confidence: 82%

Restriction endonuclease analysis of the genomes of different isolates of chicken anemia virus amplified by polymerase chain reaction

et al. 2018

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“…CAV 87/11/52 was isolated in this laboratory from clinical material obtained from a UK poultry organization (Connor et al, 1991). In the repeated passage experiment each passage involved the addition of virus-infected cells to fresh cells or growth medium at intervals of 2-3 days (McNulty et al, 1988).…”

Section: Cells and Virus Growthmentioning

confidence: 99%

“…Naturally-occurring CAV isolates, irrespective of their country of origin belong to the same serotype and produce similar pathological effects following intramuscular inoculation of day-old chicks (Connor et al, 1991;McNulty et al, 1989McNulty et al, , 1990. Experiments performed by Bulow & Fuchs (1986), have indicated that the pathogenicity of the Cux-1 isolate of CAV decreased significantly after 12 passages in MDCC-MSB1 cells and was further reduced after an additional 100 to 112 passages.…”

Section: Introductionmentioning

confidence: 99%

Molecular cloning of an attenuated chicken anaemia virus isolate following repeated cell culture passage

et al. 1995

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The pathogenicity of the Cux-1 isolate of chicken anaemia virus (CAV) was substantially reduced following large numbers (50 to 173) of passages in MDCC-MSBl cells. Restriction endonuclease analysis of polymerase chain reaction (PCR)- amplified DNAs and recombinant plasmids containing DNA inserts specified by CAV that had been passaged 173 times, indicated that the population of high-passage virus was genetically diverse. A 210-base pair (bp) insertion, containing a 19-bp sequence identical to four repeated sequences that are located in the putative non-coding region of the genome was shown to have become established in the virus population by passage number 30. Individual virus isolates that were selected from the high-passage virus population using recombinant DNA cloning and transfection methodologies varied in their pathogenicities. One cloned virus isolate, designated number 10, produced virtually no anaemia and substantially reduced levels of aplasia of the bone marrow and thymus atrophy. The pathogenicity of this isolate was restored following 10 passages in young chicks.

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“…CAA isolates which have been designated EF 88/ 78/276, 87/10/44 and 89/3713 were isolated in this laboratory from material originating in the U.S.A. [20], the U.K. [19], and Australia [3]. Hereafter these viruses will be named according to their country of origin.…”

Section: Cells and Virus Growthmentioning

confidence: 99%

Characterization of viral DNAs from cells infected with chicken anaemia agent: sequence analysis of the cloned replicative form and transfection capabilities of cloned genome fragments

Meehan¹,

Todd²,

Creelan³

et al. 1992

Archives of Virology

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The viral DNAs induced by the unclassified animal virus, chicken anaemia agent (CAA), during replication in MDCC-MSB 1 cells have been investigated. Analyses after S1 nuclease, restriction endonuclease and denaturation treatments indicated that infected cell extracts contained genome-size, single-stranded DNA (M(r) 2.3 kb), closed and open circular, double-stranded replicative form (RF) DNAs (M(r) 2.3 kbp) and a population of smaller double-stranded DNAs (M(r) 0.8 kbp). Recombinant plasmids containing 2.3 kbp CAA RF fragments cloned at the PstI, BamHI and EcoRI sites failed to transfect MDCC-MSB 1 cells. However, one plasmid, which contained two 2.3 kbp CAA RF fragments ligated in tandem at the PstI site, and cloned 2.3 kbp PstI, BamHI and EcoRI fragments, excised from their respective plasmids by restriction endonuclease digestion, were capable of transfection. The nucleotide sequence of the circular genome (2298 bp) of the Cux-1 isolate of CAA has indicated the presence of three overlapping open reading frames (ORFs) of 52 kDa, 24 kDa and 13 kDa on one strand. The existence of these ORFs was corroborated by analyses of partial sequences from three other isolates. The non-coding region of the CAA genome contained sequences with putative regulatory function. These results are discussed in relation to the "rolling circle" model of DNA replication.

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Biological characterisation of Australian isolates of chicken anaemia agent

Cited by 15 publications

References 20 publications

Restriction endonuclease analysis of the genomes of different isolates of chicken anemia virus amplified by polymerase chain reaction

Restriction endonuclease analysis of the genomes of different isolates of chicken anemia virus amplified by polymerase chain reaction

Molecular cloning of an attenuated chicken anaemia virus isolate following repeated cell culture passage

Characterization of viral DNAs from cells infected with chicken anaemia agent: sequence analysis of the cloned replicative form and transfection capabilities of cloned genome fragments

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