Biological caproate production by Clostridium kluyveri from ethanol and acetate as carbon sources

Yin, Yanan; Zhang, Yifeng; Karakashev, Dimitar Borisov; Wang, Jianlong; Angelidaki, Irini

doi:10.1016/j.biortech.2017.05.184

Cited by 108 publications

(60 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…C. kluyveri is distinguished by both its nutritional requirements and its ability to ferment ethanol to caproate [28]. Cells are motile, with peritrichous flagella, and generally occur singly, and occasionally in pairs or chains.…”

Section: Clostridium Kluyverimentioning

confidence: 99%

Clostridium sp. as Bio-Catalyst for Fuels and Chemicals Production in a Biorefinery Context

et al. 2019

View full text Add to dashboard Cite

Clostridium sp. is a genus of anaerobic bacteria capable of metabolizing several substrates (monoglycerides, diglycerides, glycerol, carbon monoxide, cellulose, and more), into valuable products. Biofuels, such as ethanol and butanol, and several chemicals, such as acetone, 1,3-propanediol, and butyric acid, can be produced by these organisms through fermentation processes. Among the most well-known species, Clostridium carboxidivorans, C. ragsdalei, and C. ljungdahlii can be highlighted for their ability to use gaseous feedstocks (as syngas), obtained from the gasification or pyrolysis of waste material, to produce ethanol and butanol. C. beijerinckii is an important species for the production of isopropanol and butanol, with the advantage of using hydrolysate lignocellulosic material, which is produced in large amounts by first-generation ethanol industries. High yields of 1,3 propanediol by C. butyricum are reported with the use of another by-product from fuel industries, glycerol. In this context, several Clostridium wild species are good candidates to be used as biocatalysts in biochemical or hybrid processes. In this review, literature data showing the technical viability of these processes are presented, evidencing the opportunity to investigate them in a biorefinery context.

show abstract

Section: Clostridium Kluyverimentioning

confidence: 99%

Clostridium sp. as Bio-Catalyst for Fuels and Chemicals Production in a Biorefinery Context

et al. 2019

View full text Add to dashboard Cite

show abstract

“…C arbon chain elongation (CCE) was recently highlighted as a promising biorefinery technology for converting hydrophilic short-chain carboxylates (SCCAs; C 2 to C 5 ) derived from biowaste into more valuable and hydrophobic medium-chain carboxylates (MCCAs; mainly C 6 or C 8 ), which serve as precursors for a range of promising biorefinery products (1)(2)(3)(4)(5).…”

mentioning

confidence: 99%

Metabolic Interactions of a Chain Elongation Microbiome

Han

Shao

et al. 2018

Appl Environ Microbiol

109

View full text Add to dashboard Cite

Carbon chain elongation (CCE), a reaction within the carboxylate platform that elongates short-chain to medium-chain carboxylates by mixed culture, has attracted worldwide interest. The present study provides insights into the microbial diversity and predictive microbial metabolic pathways of a mixed-culture CCE microbiome on the basis of a comparative analysis of the metagenome and metatranscriptome. We found that the microbial structure of an acclimated chain elongation microbiome was a highly similar to that of the original inoculating biogas reactor culture; however, the metabolic activities were completely different, demonstrating the high stability of the microbial structure and flexibility of its functions. Additionally, the fatty acid biosynthesis (FAB) pathway, rather than the well-known reverse β-oxidation (RBO) pathway for CCE, was more active and pivotal, though the FAB pathway had more steps and consumed more ATP, a phenomenon that has rarely been observed in previous CCE studies. A total of 91 draft genomes were reconstructed from the metagenomic reads, of which three were near completion (completeness, >97%) and were assigned to unknown strains of ,, and The last two strains are likely new-found active participators of CCE in the mixed culture. Finally, a conceptual framework of CCE, including both pathways and the potential participators, was proposed. Carbon chain elongation means the conversion of short-chain volatile fatty acids to medium-chain carboxylates, such as -caproate and-caprylate with electron donors under anaerobic condition. This bio-reaction can both expand the resource of valuable biochemicals and broaden the utilization of low-grade organic residues in a sustainable biorefinery context. is conventionally considered model microbe for carbon chain elongation which uses the reverse β-oxidation pathway. However, little is known about the detailed microbial structure and function of other abundant microorganism in a mixed culture (or open culture) of chain elongation. We conducted the comparative metagenomic and metatranscriptomic analysis of a chain elongation microbiome to throw light on the underlying functional microbes and alternative pathways.

show abstract

“…An optimized medium including 0.85 g L −1 of sodium acetate was used for the production of 6.96 g L −1 caproic acid by C. acetobutyricum . Another batch study was performed to examine caproate production via chain elongation using different ratios of ethanol and acetate . The maximum amount of caproate obtained was 3.11 g L −1 with an ethanol:acetate ratio of 7:3.…”

Section: Anaerobic Production Of Caproatementioning

confidence: 99%

“…They are unsuitable to be used directly as fuels because of their low energy density and high oxygen‐to‐carbon ratio . Alternatively, the low‐soluble and high‐energy MCFA have been suggested as promising intermediates to be converted to value‐added fuels or chemicals …”

Section: Bess Versus Anaerobic Digestion Bioreactorsmentioning

confidence: 99%

Bioelectrochemical synthesis of caproate through chain elongation as a complementary technology to anaerobic digestion

Reddy

ElMekawy

Pant

2018

Biofuels Bioprod Bioref

View full text Add to dashboard Cite

Chain elongation is one of the common anaerobic fermentation processes in which bacteria convert ethanol and short chain fatty acids (SCFA) into medium chain fatty acids (MCFA). These are single carboxylic acids having six to twelve carbon atoms, with several applications, such as biofuels. Caproate is a promising MCFA, and several technologies were proposed for the valorization of waste to obtain it. Bioelectrochemical systems (BESs) are technologies that are capable of converting the chemical energy of organic/inorganic wastes into value‐added products, using in situ generated H2 or electricity as energy sources. To convert waste biomass into caproate, an electron donor in the form of hydrogen or ethanol should be supplemented within the anaerobic fermentation, which can be used as the electron donor in the cathodic compartment for the conversion of acetate into caproate. This review highlights recent anaerobic and bioelectrochemical processes for the production of caproate. The discussion will also cover the potential applications of this technology, together with obstacles to its use, compared with the conventional anaerobic digestion (AD) process, and considers whether it could be a standalone technology or a complementary one for AD. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd

show abstract

Biological caproate production by Clostridium kluyveri from ethanol and acetate as carbon sources

Cited by 108 publications

References 40 publications

Clostridium sp. as Bio-Catalyst for Fuels and Chemicals Production in a Biorefinery Context

Clostridium sp. as Bio-Catalyst for Fuels and Chemicals Production in a Biorefinery Context

Metabolic Interactions of a Chain Elongation Microbiome

Bioelectrochemical synthesis of caproate through chain elongation as a complementary technology to anaerobic digestion

Contact Info

Product

Resources

About