Biological behaviour and morphological characteristics of a transplantable tumour (MM-KMY) derived from a malignant meningioma in an F344 rat

Yamate, Jyoji; Tajima, Masanori; Saitoh, Toshiki; Shibuya, Kazumoto

doi:10.1016/s0021-9975(05)80003-9

Cited by 14 publications

(10 citation statements)

References 23 publications

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“…The impaired lysosomal degradation of cortical tubular epithelium, probably related to the excessive amount of filtrated protein, may lead to storage of these proteins in cytoplasmic hyaline droplets or, in more severe cases, in intracellular and luminal crystals resulting in the histopathological picture that we observed. These findings parallel those described for hyaline droplet nephropathy in association with histiocytic sarcoma in mice and rats (Hard and Snowden 1991;Luz and Murray, 1991; Yamate et al, 1997), and with transplantable fibrosarcoma and meningioma in rats (Yamate et al, 1998; Yamate et al, 1994). Hyaline droplet nephropathy, when associated with the above‐mentioned tumours is caused by accumulation and storage of lysozyme.…”

Section: Discussionsupporting

confidence: 86%

Eosinophilic Crystals as a Distinctive Morphologic Feature of a Hyaline Droplet Nephropathy in a Mouse Model of Acute Myelogenous Leukaemia

Marchesi¹,

Monestiroli

Capillo

et al. 2003

Journal of Veterinary Medicine Series A

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Eosinophilic crystals have been described in the upper and lower respiratory tract, gall bladder, intrahepatic bile ducts and glandular stomach of different laboratory mice strains. They have been recently identified as chitinase-like (Ym1/Ym2) proteins. Here we describe the occurrence of eosinophilic crystals in the renal tubules of mice with experimentally induced acute myelogenous leukaemia. Fourteen FVB/N and 29 129Sv mice of both sexes, 8-10 weeks of age, were employed to establish a model of human acute myelogenous leukaemia. Nine mice that developed a widespread acute myelogenous leukaemia revealed the presence of eosinophilic crystals in renal tubules. The presence of eosinophilic crystals in the kidneys was constantly associated with a hyaline droplet nephropathy. Immunohistochemistry showed that the crystals and the hyaline droplets were composed of chitinase-like (Ym1/Ym2) proteins. Furthermore, immunoreactivity for Ym1/Ym2 proteins was also detected in the crystalline material stored in the cytoplasm of large macrophage-like cells or in extracellular localization within the leukaemic infiltrates. On the basis of our results we hypothesize that the detection of the Ym1/Ym2 proteins in the urine of mice might represent a feasible indicator of the burden and progression of the leukaemic condition in our murine model.

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Section: Discussionsupporting

confidence: 86%

Eosinophilic Crystals as a Distinctive Morphologic Feature of a Hyaline Droplet Nephropathy in a Mouse Model of Acute Myelogenous Leukaemia

Marchesi¹,

Monestiroli

Capillo

et al. 2003

Journal of Veterinary Medicine Series A

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“…The histological differences between the primary tumor and transplanted tumors might, at least in part, be due to the inappropriate nature of subcutaneous tissues as a transplantation site with regard to the preservation of alveolar structures. Further, undifferentiated neoplastic cells might be selected in the process of transplantation; morphological alterations due to dedifferentiation of neoplastic cells have been reported in long-term serial transplantation of rat nephroblastoma [13] and malignant meningioma [44]. Therefore, with these facts and precedents in mind, we concluded that the present tumors should be regarded as alveolar/bronchiolar carcinoma.…”

Section: Discussionmentioning

confidence: 70%

Establishment of a transplantable tumor line (IP) derived from rat pulmonary carcinoma, developing humoral hypercalcemia of malignancy in IP-bearing rats

et al. 2001

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A transplantable tumor line (IP) was established in syngeneic rats from a spontaneous pulmonary carcinoma found in a male F344 rat aged 25 months. A tissue fragment of IP grew into a nodule, 2-3 cm in diameter, 2-3 weeks after implant, and IP has been serially passed through 26 generations. Lined by cuboidal or columnar epithelial cells, the primary tumor consisted of completely alveolar architecture. However, IP tumors developed various growth patterns such as glandular, acinar, trabecular, cord, and solid, and consisting of epithelial cells showing cellular atypia. Ultrastructurally, neoplastic cells had microvilli, basement membranes, and desmosomes, and occasional cells possessed dense cytoplasmic granules. Interestingly, it was shown by the immunohistochemistry, the RT-PCR method, and immunoradiometric assay that IP tumor cells produce parathyroid hormone-related protein (PTHrP). During a 3-week observation period after implant, IP-bearing rats showed severe emaciation, hypercalcemia, and hypophosphatemia, as well as an increase in osteoclastic areas and a decrease in shaft thickness of the femurs. These were considered to be due to a marked elevation of plasma PTHrP levels. Furthermore, IP-bearing rats developed calcification in various organs including the kidneys, lungs, and heart. These findings in IP-bearing rats were similar to those of humoral hypercalcemia of malignancy (HHM) reported in human cancer patients. PTHrP plays a central role in the development of HHM, but the mechanisms of HHM remain poorly understood. IP may become a useful model for studying the pathogenesis of HHM and the pathophysiological role of PTHrP.

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“…A tissue fragment 2 mm in diameter was aseptically cut from the primary tumour, which was later diagnosed as fibrosarcoma by pathological examinations. The tissue fragment was transplanted subcutaneously into a syngeneic male rat through a trochar under ether anaesthesia [38,39]. Five weeks after the implantation, a subcutaneous tumour developed into a nodule weighing 3 g. A portion of the subcutaneous tumour was used for transplantation in the second generation.…”

Section: Methodsmentioning

confidence: 99%

“…Primary antibodies used are shown in Table 1. The detailed procedures have been described elsewhere [39,41]. Briefly, after treatments with 0.1% trypsin solution and then with 3% H 2 O 2 , the sections were incubated for 14 h at 4° C with the primary antibodies listed in Table 1.…”

Section: Methodsmentioning

confidence: 99%

“…Rat tissues were used as positive controls [38,39,41], and sections treated with nonimmune mouse or rabbit serum instead of primary antibody, as negative controls. Frozen sections from fresh tumour tissues were stained for acid phosphatase (ACP; Gomori's method, pH 5.0), nonspecific esterase (NSE; alpha-naphtyl acetate method, pH 7.4) and alkaline phosphatase (ALP; naphthol AS method, pH 9.0) by the same enzyme-histochemical methods as described previously [38,39,41].…”

Section: Methodsmentioning

confidence: 99%

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Characteristics of a rat fibrosarcoma-derived transplantable tumour line (SS) and cultured cell lines (SS-P and SS-A3-1) showing myofibroblastic and histiocytic phenotypes

et al. 1997

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A transplantable tumour line (SS) was established in syngeneic rats from a spontaneous fibrosarcoma that had arisen in the submandibular salivary gland of a 24-month-old male F344 rat. A cell line (SS-P) was induced from SS, and a cloned cell line (SS-A3-1) was isolated from SS-P. The primary tumour consisted of oval to spindle-shaped cells arranged in bundles with abundant collagen fibres; ultrastructurally, neoplastic cells exhibited fusiform morphology with prominent rough endoplasmic reticulum. SS tumours showed marked interlacing fascicle and herring-bone growth patterns. SS-P and SS-A3-1 were similar morphologically to each other, consisting of oval, spindle or polygonal cells and occasional multinucleated giant cells. Tumours induced by SS-P and SS-A3-1 were histologically similar to SS tumours. Immunohistochemically, all cells in the primary tumour, SS tumours and tumours induced both by SS-P and SS-A3-1 and by SS-P and SS-A3-1 cultures gave a positive reaction to vimentin. Interestingly, neoplastic cells reacting to ED1 (rat macrophage/histiocyte-specific antibody) and alpha-smooth muscle actin (alpha-SMA) appeared in SS tumours and tumours induced by SS-P and SS-A3-1 and by SS-P and SS-A3-1 cultures. Cells with histiocytic fine structures and myofibroblastic cells with cytoplasmic actin-like microfilaments were also observed by electron microscopy. The present rat fibrosarcoma-derived transplantable tumour line (SS) and cell lines (SS-P and SS-A3-1) might express myofibroblastic and histiocytic phenotypes, probably depending on the surrounding conditions. These cell lines may prove useful for studying the mechanisms of phenotypic plasticity in neoplastic fibroblasts.

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Biological behaviour and morphological characteristics of a transplantable tumour (MM-KMY) derived from a malignant meningioma in an F344 rat

Cited by 14 publications

References 23 publications

Eosinophilic Crystals as a Distinctive Morphologic Feature of a Hyaline Droplet Nephropathy in a Mouse Model of Acute Myelogenous Leukaemia

Eosinophilic Crystals as a Distinctive Morphologic Feature of a Hyaline Droplet Nephropathy in a Mouse Model of Acute Myelogenous Leukaemia

Establishment of a transplantable tumor line (IP) derived from rat pulmonary carcinoma, developing humoral hypercalcemia of malignancy in IP-bearing rats

Characteristics of a rat fibrosarcoma-derived transplantable tumour line (SS) and cultured cell lines (SS-P and SS-A3-1) showing myofibroblastic and histiocytic phenotypes

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