Biological applications of second harmonic imaging

Cox, Guy

doi:10.1007/s12551-011-0052-9

Cited by 39 publications

(29 citation statements)

References 42 publications

(49 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Second harmonic generation (SHG) microscopy has emerged as a powerful nonlinear optical imaging technique in the last decade (Cox 2011). SHG is a nonlinear optical effect observed in non-centrosymmetric molecules, where two photons with the same frequency "combine" to produce a single photon with twice the frequency (Cox 2011). This process has been used to obtain images of SHG active molecules by subjecting the specimen to highly focused, short pulse of radiation and recording the emitted signal.…”

Section: Second Harmonic Generation Microscopy Of Starch Granulesmentioning

confidence: 99%

Advanced microscopy techniques for revealing molecular structure of starch granules

et al. 2020

View full text Add to dashboard Cite

Starch is a major source of our daily diet and it is important to understand the molecular structure that plays a significant role in its wide number of applications. In this review article, microscopic structures of starch granules from potato, corn, rice canna, tania, wheat, sweet potato, and cassava are revealed using advanced microscopic techniques. Optical microscopy depicts the size and shape, polarization microscopy shows the anisotropy properties of starch granules, scanning electron microscopy (SEM) displays surface topography, and confocal microscopy is used to observe the three-dimensional internal structure of starch granules. The crystallinity of starch granules is revealed by second harmonic generation (SHG) microscopy and atomic force microscopy (AFM) provides mechanical properties including strength, texture, and elasticity. These properties play an important role in understanding the stability of starch granules under various processing conditions like heating, enzyme degradation, and hydration and determining its applications in various industries such as food packaging and textile industries.

show abstract

Section: Second Harmonic Generation Microscopy Of Starch Granulesmentioning

confidence: 99%

Advanced microscopy techniques for revealing molecular structure of starch granules

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Second harmonic generation (SHG) microscopy is a frequency-doubling technique 5 . The incident light sent to a sample is coherent: two photons of the same frequency are used with the photons in 'lock-step' , in phase with one another.…”

Section: Getting Some Glowmentioning

confidence: 99%

It’s free imaging — label-free, that is

Marx

2019

Nat Methods

View full text Add to dashboard Cite

“…In addition, asymmetric molecules such as collagen type I and elastin can produce light at exactly twice the frequency (or half the wavelength) of the pulsed excitation beam, a feature that is referred to as second harmonic generation (SHG) [ 21 , 25 ]. SHG does not suffer from photobleaching and allows for extended periods of observation [ 26 ]. Moreover, the two-photon laser excitation beams can penetrate deeper into the tissue allowing imaging and tracking of cells in relatively thick samples of up to 1 mm [ 20 , 27 , 28 ].…”

Section: Introductionmentioning

confidence: 99%

Label‐Free Imaging of Umbilical Cord Tissue Morphology and Explant‐Derived Cells

Donders

Sanen

Paesen

et al. 2016

Stem Cells International

View full text Add to dashboard Cite

In situ detection of MSCs remains difficult and warrants additional methods to aid with their characterization in vivo. Two-photon confocal laser scanning microscopy (TPM) and second harmonic generation (SHG) could fill this gap. Both techniques enable the detection of cells and extracellular structures, based on intrinsic properties of the specific tissue and intracellular molecules under optical irradiation. TPM imaging and SHG imaging have been used for label-free monitoring of stem cells differentiation, assessment of their behavior in biocompatible scaffolds, and even cell tracking in vivo. In this study, we show that TPM and SHG can accurately depict the umbilical cord architecture and visualize individual cells both in situ and during culture initiation, without the use of exogenously applied labels. In combination with nuclear DNA staining, we observed a variance in fluorescent intensity in the vessel walls. In addition, antibody staining showed differences in Oct4, αSMA, vimentin, and ALDH1A1 expression in situ, indicating functional differences among the umbilical cord cell populations. In future research, marker-free imaging can be of great added value to the current antigen-based staining methods for describing tissue structures and for the identification of progenitor cells in their tissue of origin.

show abstract

Biological applications of second harmonic imaging

Cited by 39 publications

References 42 publications

Advanced microscopy techniques for revealing molecular structure of starch granules

Advanced microscopy techniques for revealing molecular structure of starch granules

It’s free imaging — label-free, that is

Label‐Free Imaging of Umbilical Cord Tissue Morphology and Explant‐Derived Cells

Contact Info

Product

Resources

About