Biological applications of phytosynthesized gold nanoparticles using leaf extract of Dracocephalum kotschyi

Abstract

In this work, biosynthesis potentials of Dracocephalum kotschyi leaf extract for the production of gold nanoparticle (AuNPs) were studied, and the biological (catalytic, antibacterial, antioxidant, and anticancer) activities of studied AuNPs were evaluated. Different analytical techniques including UV–visible spectroscopy, X‐ray diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM), energy dispersive X‐ray analysis, and transmission electron microscopy (TEM) were used for the characterization of AuNPs. Moreover, Different testing methods were used for evaluating biological activities of biosynthesized AuNPs. The formation of AuNPs was confirmed by color change and UV–visible spectroscopic analysis. Field emission (FE)‐SEM and TEM images were used to characterize phytosynthesized AuNPs which were predominantly spherical in shape with size in the range of 5–21 nm. These spherical NPs were found to be 39.79 ± 5 nm in size as determined by dynamic light scattering particle size analyzer. XRD pattern confirms the crystalline nature of the biosynthesized nanoparticles. The phytoconstituents involved in the reduction and stabilization of nanoparticles have been identified using FTIR spectra. The phytosynthesized AuNPs showed effective antioxidant, antibacterial and catalytic reduction activities. Furthermore, they have inhibited H1229 and MCF‐7 cancer cell lines proliferation in a dose‐dependent manner. These results have supported that D. kotschyi leaf extract was very efficient for the synthesis of AuNPs, and synthesized NPs showed enhanced biological activities which make them suitable for biomedical applications. © 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 107A: 621–630, 2019.

Biological and Catalytic Activities of Green Synthesized Silver Nanoparticles from the Leaf Infusion of Dracocephalum kotschyi Boiss

“…Our previous study on antibacterial effects of AgNPs synthesized by Nigella arevensis leaf extract with mostly spherical shape and size‐range of 5–100 nm [ 23 ] showed the inhibition zone of 17 mm and MIC value of 7.82 μg mL −1 for E. coli , which are significantly weaker than our finding in the current study. In another study, we showed that gold nanoparticles synthesized by D. Kotschyi leaf extract also have significant antibacterial effects against B. subtilis , [ 22 ] indicating that D. Kotschyi leaf extract has a synergistic impact on antibacterial effects of NPs. D. Kotschyi leaf extract mediated NPs have better antibacterial effects comparing to similar NPs from different sources.…”

“…The infusion was cooled, centrifuged, and filtered by filter paper to get a transparent aqueous extract, then stored at 4 °C for further use. [ 22 ]…”

“…Untreated cells were considered as control. Cell viability was calculated by the following formula: [ 22 ] …”

Treatment with Gold Nanoparticles Using Cudrania tricuspidata Root Extract Induced Downregulation of MMP-2/-9 and PLD1 and Inhibited the Invasiveness of Human U87 Glioblastoma Cells

“…There is a lot of information available in the literature regarding the molecular targets underlying the anti-tumor properties of biocompatible GNPs [21,22]. GNP-based plants have been experimentally documented to possess medicinal properties as well as various biological properties such as antioxidant, anti-bacterial, anti-inflammatory, and anti-tumor activities [23][24][25]. Cudrania tricuspidata is a perennial plant of the Moraceae family, which is mainly distributed in Asian countries such as Korea, China, and Japan.…”

One‐pot facile synthesis of silk sericin‐capped gold nanoparticles by UVC radiation: Investigation of stability, biocompatibility, and antibacterial activity

“…Despite these important properties, there are also studies emphasizing the in vitro or in vivo toxic effects of AuNPs, synthesized especially by means of chemical procedures (Gerber, Bundschuh, Klingelhofer, & Groneberg, ; Jia, Ma, Wei, & Qian, ; Xia et al, ). The so‐called “green synthesis” methods involving biological macromolecules, such as silk fibroin (Jia, Guo, Zhu, & Ma, ), chitosan (Yang & Li, ), and leaf extracts (Chahardoli, Karimi, Fattahi, & Salimikia, ) have been introduced recently to alleviate the toxicity‐related problems as well as to improve the colloidal stability of AuNPs. There is also no need to use potentially toxic reducing and/or stabilizing agents for the synthesis reactions since biological macromolecules could act as both reducing and stabilizing agents (Jia et al, ; Yang & Li, ).…”