Biological applications of microfluidic gradient devices

Kim, Sudong; Kim, Hyung Joon; Jeon, Noo Li

doi:10.1039/c0ib00055h

Cited by 321 publications

(283 citation statements)

References 137 publications

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“…By proving its effectiveness, gradient microfluidic have been widely used for cell biology applications (Seidi et al, 2011;Nitta et al, 2009), especially on cell chemotaxis and migration research (Li et al, 2012;Brett et al, 2012;Kim et al, 2013;Meier et al, 2011;Raja et al, 2010). By providing experimental platforms that allow systematic investigation and quantitative analysis, microfluidic devices have brought a new level of understanding that was not available with the large-scale devices of the past (Kim et al, 2010).…”

Section: B)mentioning

confidence: 99%

“…:þ86-13883821386 -1058Tel. :þ86-13883821386 /2014(1)/014108/8/$30.00 V C 2014 AIP Publishing LLC 8, 014108-1 studying chemotaxis and axon guidance (Falasca et al, 2011;Chung and Chen, 2009;Walheim et al, 2012), but these platforms are typically limited to simple, non-quantitative gradients that cannot be actively altered or precisely controlled in a reproducible fashion (Kim et al, 2010;Chung and Choo, 2010). Therefore, there is a growing need for generating a more robust and stable molecular gradient.…”

Section: B)mentioning

confidence: 99%

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Using microfluidic chip to form brain-derived neurotrophic factor concentration gradient for studying neuron axon guidance

Huang

Jiang

et al. 2014

Biomicrofluidics

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Molecular gradients play a significant role in regulating biological and pathological processes. Although conventional gradient-generators have been used for studying chemotaxis and axon guidance, there are still many limitations, including the inability to maintain stable tempo-spatial gradients and the lack of the cell monitoring in a real-time manner. To overcome these shortcomings, microfluidic devices have been developed. In this study, we developed a microfluidic gradient device for regulating neuron axon guidance. A microfluidic device enables the generation of Brain-derived neurotrophic factor (BDNF) gradient profiles in a temporal and spatial manner. We test the effect of the gradient profiles on axon guidance, in the BDNF concentration gradient axon towards the high concentration gradient. This microfluidic gradient device could be used as a powerful tool for cell biology research. V C 2014 AIP Publishing LLC.

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Section: B)mentioning

confidence: 99%

Section: B)mentioning

confidence: 99%

Using microfluidic chip to form brain-derived neurotrophic factor concentration gradient for studying neuron axon guidance

Huang

Jiang

et al. 2014

Biomicrofluidics

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“…To better reproduce physiological and pathological phenomena in a microfluidic device, researchers have made great efforts to mimic cellular microenvironments. [25][26][27][28] Many microfluidic-based devices designed to explore the effect of oxygen tension on cell migration have been reported. [29][30][31][32][33][34][35][36] In one example, a three-dimensional microfluidic cell culture system featuring a controlled hypoxia environment was developed, a central 3D gel region acting as an external cellular matrix, and peripheral gas channel on each side establishing oxygen environment.…”

Section: Introductionmentioning

confidence: 99%

A novel microfluidic platform for studying mammalian cell chemotaxis in different oxygen environments under zero-flow conditions

et al. 2015

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The cell's micro-environment plays an important role in various physiological and pathological phenomena. To better investigate in vivo cellular behaviors, researchers have expended great effort in building controlled in vitro biophysical and biochemical environments. Because a cell's gaseous environment affects properties such as its division, metastasis, and differentiation, we developed a zero-flow based platform for studying mammalian cell chemotaxis behavior in different oxygen environments. This platform can construct a linear range of oxygen tensions within one chip (i.e., from 1.4% to 3.6% or 5.5% to 14.5%). To study cell chemotaxis behavior under varying oxygen environments, the chemical gradient direction is established perpendicularly to oxygen change within an observation area. Because the observation area is not subject to flow, shear force is of no concern. In addition, water flow around the cell chambers greatly reduces evaporation and makes long-term microscope imaging possible. In this study, we precisely measure the chemotaxis velocity of MCF-7 human breast cancer cells under different oxygen tension conditions towards CXCL12, which is a stromal cell-derived factor. We find that cell migration rates are not equivalent, even under two close oxygen tensions. We also observed that cells move faster towards high concentrations of chemoattractant when the oxygen tension is below 3% due to the increased expression of HIF-1 (hypoxia-inducible factor 1), which promotes a transition to the amoeboid rather than mesenchymal mode of movement. Our experiments demonstrate that this new microfluidic platform is useful for the quantitative study of mammalian cell chemotaxis under different oxygen conditions in the absence of shear force. We also shed light on the study of chemotaxis under other gaseous environments. V C 2015 AIP Publishing LLC.

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“…[12][13][14][15][16][17][18][19][20] Moreover, a microfluidic device capable of precisely positioning EBs to study their differentiation under various controlled a) Author to whom correspondence should be addressed. Electronic mail: tungy@gate.sinica.edu.tw.…”

Section: Introductionmentioning

confidence: 99%

Flip channel: A microfluidic device for uniform-sized embryoid body formation and differentiation

2015

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This paper reports a two-layered polydimethylsiloxane microfluidic device-Flip channel, capable of forming uniform-sized embryoid bodies (EBs) and performing stem cell differentiation within the same device after flipping the microfluidic channel. The size of EBs can be well controlled by designing the device geometries, and EBs with multiple sizes can be formed within a single device to study EB size-dependent stem cell differentiation. During operation of the device, cells are positioned in the designed positions. As a result, observation and monitoring specific population of cells can be achieved for further analysis. In addition, after flipping the microfluidic channel, stem cell differentiation from the EBs can be performed on an unconfined flat surface that is desired for various differentiation processes. In the experiments, murine embryonic stem cells (ES-D3) are cultured and formed EBs inside the developed device. The size of EBs is well controlled inside the device, and the neural differentiation is performed on the formed EBs after flipping the channel. The EB size-dependent stem cell differentiation is studied using the device to demonstrate its functions. The device provides a useful tool to study stem cell differentiation without complicated device fabrication and tedious cell handling under better-controlled microenvironments.

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Biological applications of microfluidic gradient devices

Cited by 321 publications

References 137 publications

Using microfluidic chip to form brain-derived neurotrophic factor concentration gradient for studying neuron axon guidance

Using microfluidic chip to form brain-derived neurotrophic factor concentration gradient for studying neuron axon guidance

A novel microfluidic platform for studying mammalian cell chemotaxis in different oxygen environments under zero-flow conditions

Flip channel: A microfluidic device for uniform-sized embryoid body formation and differentiation

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