Biological application of microelectrode arrays in drug discovery and basic research

Stett, Alfred; Egert, Ulrich; Guenther, Elke; Hofmann, Frank; Meyer, Thomas; Nisch, W.; Haemmerle, H.

doi:10.1007/s00216-003-2149-x

Cited by 422 publications

(272 citation statements)

References 48 publications

Supporting

Mentioning

253

Contrasting

Order By: Relevance

“…Previously, the FPs have been shown to correlate well with the underlying cellular electrophysiology [8], and this arrangement allowed for evaluation of changes in the dominant pacemaker, a surrogate for ectopic activity, and of conduction velocity [9,10]. Mapping of two-dimensional cardiomyocytes cultures has proven an efficient methodology for exploring arrhythmic risk [9][10][11][12][13][14][15][16].…”

Section: Resultsmentioning

confidence: 99%

A possible mechanism of halocarbon-induced cardiac sensitization arrhythmias

Jing

Jesús

Iravanian

et al. 2006

Journal of Molecular and Cellular Cardiology

View full text Add to dashboard Cite

Cardiac sensitization is the term used for malignant ventricular arrhythmias associated with exposure to inhaled halocarbons in the presence of catecholamines. We investigated the electrophysiological changes associated with cardiomyocyte exposure to epinephrine and a halocarbon known to be associated with cardiac sensitization (halon 1301, CF 3 Br). Cardiomyocytes (CMs) were isolated from neonatal rats and grown on multielectrode arrays (MEAs). Upon exposure to epinephrine, the CM inter-spike interval (ISI) was decreased 14% at 10 µg/L (P<0.05) and 27% at 100 µg/L (P<0.05) as compared to baseline. Halon alone (50 mg/L) mildly prolonged the field potential (FP) duration (7%). CMs exposed to combinations of epinephrine (100 µg/L) and halon (50 mg/L) for 15 min showed a blunted increase in the ISI (35±12%) and a 38% decrease in conduction velocity (P<0.05) when compared to epinephrine alone. There was no change in field potential properties, but dephosphorylated connexin 43 (Cx43) was increased 60±16% with the combination as compared to epinephrine alone (P<0.05). Treatment with okadaic acid, a phosphatase inhibitor, prevented the Cx43 dephosphorylation and the reduction in conduction velocity upon exposure to halon and epinephrine. Moreover, the electrophysiological changes induced by epinephrine and halon were indistinguishable from those seen with the gap junction inhibitor heptanol. In conclusion, the combination of a halocarbon and epinephrine results in a unique electrophysiological signature including slow conduction that may explain, in part, the basis for cardiac sensitization. The slowing of conduction is most likely related to changes in the phosphorylation state of Cx43.

show abstract

Section: Resultsmentioning

confidence: 99%

A possible mechanism of halocarbon-induced cardiac sensitization arrhythmias

Jing

Jesús

Iravanian

et al. 2006

Journal of Molecular and Cellular Cardiology

View full text Add to dashboard Cite

show abstract

“…It is well-suited for studying synaptic plasticity [21,[31][32][33] , single unit activity [49,50] , rhythmic activity [17,45,51] , and pharmacological drug testing [23,46,52] . Furthermore, powerful analytical softwares (e.g.…”

Section: Examples Of Ltd Recording Using Meas Besidesmentioning

confidence: 99%

Use of multi-electrode array recordings in studies of network synaptic plasticity in both time and space

Liu

Chen

et al. 2012

Neurosci. Bull.

View full text Add to dashboard Cite

Simultaneous multisite recording using multi-electrode arrays (MEAs) in cultured and acutely-dissociated brain slices and other tissues is an emerging technique in the field of network electrophysiology. Over the past 40 years, great efforts have been made by both scientists and commercial concerns, to advance this technique. The MEA technique has been widely applied to many regions of the brain, retina, heart and smooth muscle in various studies at the network level.The present review starts from the development of MEA techniques and their uses in brain preparations, and then specifically concentrates on the use of MEA recordings in studies of synaptic plasticity at the network level in both the temporal and spatial domains. Because the MEA technique helps bridge the gap between single-cell recordings and behavioral assays, its wide application will undoubtedly shed light on the mechanisms underlying brain functions and dysfunctions at the network level that remained largely unknown due to the technical difficulties before it matured.

show abstract

“…Applications for treatment of several kinds of diseases or disorders such as loss of sensory perception [1,2,3] central nervous system 25 disorders or loss of limbs [4,5,6] would all benefit from bidirectional communication with electrogenic networks of cells. Micro electrode arrays (MEAs) have been established as an electrical recording system that is non-invasive for 30 electrogenic cells [7]. MEA recording does not disturb cell signaling and generates scar tissue only upon initial placement.…”

Section: Introductionmentioning

confidence: 99%