Biological and proteomic characterization of a composite mesh for abdominal wall hernia treatment: Reference Study

Abstract

CMC shows a good cell biocompatibility and growth. The increase of pro-inflammatory markers could stimulate proliferation, influencing the integration process in human body. Proteomics highlights the ECM modulation by CMC. An integrated investigation of these biological analyses with mechanical data should improve the design process of a new product. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 105B: 2045-2052, 2017.

Laparoscopy for the Treatment of Congenital Hernia: Use of Surgical Meshes and Mesenchymal Stem Cells in a Clinically Relevant Animal Model

“…First, there is a lack of uniformity regarding the use of animal models in preclinical trials (Vogels et al, 2017). Even the most recent studies that have investigated the use of stem cells on surgical meshes have been performed in vitro (Gao et al, 2014;Vozzi et al, 2017) or in small animal models, especially in mice (Darzi et al, 2018;Paul et al, 2019;Mukherjee et al, 2020), rats (Altman et al, 2010a;Altman et al, 2010b;Edwards et al, 2015;Iyyanki et al, 2015;Klinger et al, 2016;van Steenberghe et al, 2017;Hansen et al, 2020), and rabbits (Zhao et al, 2012;Cheng et al, 2017). Only a few preclinical studies have been performed in sheep (Gerullis et al, 2013;Gerullis et al, 2014).…”

Integration of Biomechanical and Biological Characterization in the Development of Porous Poly(caprolactone)-Based Membranes for Abdominal Wall Hernia Treatment

“…This work was aimed at the preparation and characterization of porous PCL membranes to be used as a new generation of resorbable hernia meshes. Particularly, porous PCL membranes were characterized for their physicochemical, mechanical, and biological characteristics, comparing results with the performance of a commercially available CMC mesh: this lightweight polypropylene mesh is able to favor cell colonization, generating a series of biochemical modifications in terms of typical inflammatory reaction, collagen production, and ECM expression [26]. However, the lightweight mesh is not suitable to prevent adhesion to internal organs [34].…”

“…Cells were seeded on PCL7-2i membranes and CMC mesh and cultured for 7 days. Cells were removed and ECM recovered as described in [26].…”

“…Tandem Mass Spectrometry with SWATH TM -Based Analysis (LC-MS/MS SWATH) for Proteomics. For sample treatment and LC-MS/MS analysis, the same protocol described in Vozzi et al [26] was used. Briefly, protein concentration was determined by bicinchoninic acid assay (Pierce, Thermo Scientific, Waltham, MA, USA).…”

“…In this work, polymeric blends based on PCL with poly (ethylene glycol) (PEG) of two different molecular weights (PEG1: M w 10,000 Da; PEG2: M w 35,000 Da) were developed for application in hernia treatment and tested for their structural, mechanical, and biological properties compared to a commercial product (CMC, Dipro Medical Devices, San Mauro Torinese, Italy), used as a reference system [26]. The CMC is a nonresorbable composite prosthesis used for ventral hernia repair and made up by a polypropylene lightweight macroporous mesh sewn on a smooth polypropylene film.…”