Biological and Physicochemical Characteristics of 2 Different Hydrophilic Surfaces Created by Saline-Storage and Ultraviolet Treatment

Ghassemi, Amirreza; Ishijima, Manabu; Hasegawa, Masakazu; Rezaei, Naser Mohammadzadeh; Nakhaei, Kourosh; Sekiya, Takeo; Torii, Yasuyoshi; Hirota, Makoto; Park, Won-Hee; Miley, D. Douglas; Ogawa, Takahiro

doi:10.1097/id.0000000000000773

Cited by 19 publications

(10 citation statements)

References 31 publications

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“…Several studies have found that the inevitable storage of Ti implants in sterile, gas-permeable packaging during commercial distribution can result in substantially reduced protein adsorption capacity, chemotaxis-like remote attracting and adhering capabilities for osteoblasts, and changes in other bioactivity parameters relevant to osteoblast function, regardless of the surface topography [57,58]. The approaches used in this study enhanced the hydrophilicity of implants through producing clean surfaces.…”

Section: Discussionmentioning

confidence: 91%

Maintenance and Restoration Effect of the Surface Hydrophilicity of Pure Titanium by Sodium Hydroxide Treatment and its Effect on the Bioactivity of Osteoblasts

et al. 2019

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In recent years, studies on the surface of titanium implants have shown that hydrophilic properties have a positive effect on bone binding, warranting further investigation into the maintenance and restoration of hydrophilic properties. In this work, a hydrophilic surface was obtained by plasma oxidation on the surface of sandblasted and acid-etched (SLA) titanium discs. We aimed to determine the effect of sodium hydroxide (NaOH) treatment on the maintenance and restoration of the surface hydrophilicity of titanium discs, as well as the relationship between the changes in hydrophilic properties on titanium surfaces and their biological properties. The results show that the treatment of hydrophilic surfaces with SLA, plasma oxidation, and NaOH treatments tend to enhance the early stages of cell adhesion, proliferation, and differentiation. Those results provide important guidance that SLA, plasma oxidation, and NaOH treatments can be used to restore the hydrophilic property of Ti that has been stored under room temperature and atmospheric pressure conditions.

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Section: Discussionmentioning

confidence: 91%

Maintenance and Restoration Effect of the Surface Hydrophilicity of Pure Titanium by Sodium Hydroxide Treatment and its Effect on the Bioactivity of Osteoblasts

et al. 2019

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“…The decarbonized Ti microfiber becomes a superhydrophilic surface, which attracts and enables osteoblasts to attach and spread even on these thin and rounded fibers (Figure 9). Osteoblast activity on superhydrophilic Ti surfaces by UV treatment is greater than that on saline-stored Ti surfaces [19]. Osteoblast attachment on the Ti surface is established within at most 24 h [20,21].…”

Section: Discussionmentioning

confidence: 95%

Tuning of Titanium Microfiber Scaffold with UV-Photofunctionalization for Enhanced Osteoblast Affinity and Function

Iwasaki

Hirota

Tanaka

et al. 2020

IJMS

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Titanium (Ti) is an osteoconductive material that is routinely used as a bulk implant to fix and restore bones and teeth. This study explored the effective use of Ti as a bone engineering scaffold. Challenges to overcome were: (1) difficult liquid/cell infiltration into Ti microfiber scaffolds due to the hydrophobic nature of Ti; and (2) difficult cell attachment on thin and curved Ti microfibers. A recent discovery of UV-photofunctionalization of Ti prompted us to examine its effect on Ti microfiber scaffolds. Scaffolds in disk form were made by weaving grade 4 pure Ti microfibers (125 µm diameter) and half of them were acid-etched to roughen the surface. Some of the scaffolds with original or acid-etched surfaces were further treated by UV light before cell culture. Ti microfiber scaffolds, regardless of the surface type, were hydrophobic and did not allow glycerol/water liquid to infiltrate, whereas, after UV treatment, the scaffolds became hydrophilic and immediately absorbed the liquid. Osteogenic cells from two different origins, derived from the femoral and mandibular bone marrow of rats, were cultured on the scaffolds. The number of cells attached to scaffolds during the early stage of culture within 24 h was 3–10 times greater when the scaffolds were treated with UV. The development of cytoplasmic projections and cytoskeletal, as well as the expression of focal adhesion protein, were exclusively observed on UV-treated scaffolds. Osteoblastic functional phenotypes, such as alkaline phosphatase activity and calcium mineralization, were 2–15 times greater on UV-treated scaffolds, with more pronounced enhancement on acid-etched scaffolds compared to that on the original scaffolds. These effects of UV treatment were associated with a significant reduction in atomic carbon on the Ti microfiber surfaces. In conclusion, UV treatment of Ti microfiber scaffolds tunes their physicochemical properties and effectively enhances the attachment and function of osteoblasts, proposing a new strategy for bone engineering.

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“…UV light treatment has been reported to be an effective tool for cleaning accumulated organic contamination on medical devices. UV-treatment can remove hydrocarbon species from the surface of titanium and enhance bone conductivity [32][33][34][35][36]. Furthermore, photofunctionalization has yielded positive results in clinical studies [37,38].…”

Section: Discussionmentioning

confidence: 99%

UV-Photofunctionalization of Titanium Promotes Mechanical Anchorage in A Rat Osteoporosis Model

Taniyama

Saruta

Rezaei

et al. 2020

IJMS

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Effects of UV-photofunctionalization on bone-to-titanium integration under challenging systemic conditions remain unclear. We examined the behavior and response of osteoblasts from sham-operated and ovariectomized (OVX) rats on titanium surfaces with or without UV light pre-treatment and the strength of bone-implant integration. Osteoblasts from OVX rats showed significantly lower alkaline phosphatase, osteogenic gene expression, and mineralization activities than those from sham rats. Bone density variables in the spine were consistently lower in OVX rats. UV-treated titanium was superhydrophilic and the contact angle of ddH2O was ≤5°. Titanium without UV treatment was hydrophobic with a contact angle of ≥80°. Initial attachment to titanium, proliferation, alkaline phosphatase activity, and gene expression were significantly increased on UV-treated titanium compared to that on control titanium in osteoblasts from sham and OVX rats. Osteoblastic functions compromised by OVX were elevated to levels equivalent to or higher than those of sham-operated osteoblasts following culture on UV-treated titanium. The strength of in vivo bone-implant integration for UV-treated titanium was 80% higher than that of control titanium in OVX rats and even higher than that of control implants in sham-operated rats. Thus, UV-photofunctionalization effectively enhanced bone-implant integration in OVX rats to overcome post-menopausal osteoporosis-like conditions.

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Biological and Physicochemical Characteristics of 2 Different Hydrophilic Surfaces Created by Saline-Storage and Ultraviolet Treatment

Abstract: Despite similar hydrophilicity, saline-stored and UV light-treated surfaces showed substantially different biological effects on osseointegration, associated with different surface chemistry and morphology.

Cited by 19 publications

References 31 publications

Maintenance and Restoration Effect of the Surface Hydrophilicity of Pure Titanium by Sodium Hydroxide Treatment and its Effect on the Bioactivity of Osteoblasts

Maintenance and Restoration Effect of the Surface Hydrophilicity of Pure Titanium by Sodium Hydroxide Treatment and its Effect on the Bioactivity of Osteoblasts

Tuning of Titanium Microfiber Scaffold with UV-Photofunctionalization for Enhanced Osteoblast Affinity and Function

UV-Photofunctionalization of Titanium Promotes Mechanical Anchorage in A Rat Osteoporosis Model

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