Biological and molecular studies on Syrian hamster intracisternal R-type particles

Lesser, J.; Bittoun, Patricia; Emanoil-Ravier, Rodica; Périès, J.

doi:10.1007/bf01309726

Archives of Virology

1995

DOI: 10.1007/bf01309726

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Biological and molecular studies on Syrian hamster intracisternal R-type particles

J. Lesser

Patricia Bittoun

Rodica Emanoil-Ravier

et al.

Abstract: Retrovirus-like intracisternal R-type particles (IRP) are structures present in Syrian hamster (Mesocricetus auratus) cells cultured in vitro where they appear either spontaneously or under chemical induction conditions. We have tested several chemical inducers and ten different cell lines, looking for the best IRP induction conditions. BHK21 cl. 13 showed the highest inducibility one day after a 24 h treatment with 1 microgram/ml of 5-aza-2'-deoxycytidine. Using detergent treatments and sucrose gradients, we … Show more

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“…The presence of mouse mammary tumour viruslike retrotransposons in the genomes of Djungarian hamsters and other closely related animals was repeatedly demonstrated (Vasetskii et al 1989;Tikhonenko et al 1990;Vasetskii 1994;Lesser et al 1995;Hsu et al 2010;Xu et al 2012). Thus, more experiments to detect and amplify MMTV sequences in DNA isolated from FFPE samples as described are necessary.…”

mentioning

confidence: 88%

Familial incidence of mammary gland tumours in the Djungarian hamster (Phodopus sungaros): a case report

Jelínek¹,

Felsberg²,

Měšťan³

2013

Vet. Med. - Czech

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Tumours of the mammary gland were diagnosed in one female Djungarian hamster (Phodopus sungaros) and in two of her daughters from two litters. Altogether, five tumours were diagnosed. Three of them were adenocarcinomas, one was adenoma with disseminated foci of adenocarcinoma and one was diagnosed as an atypical fibrosarcoma derived from cutaneous ganglion cell-like cells. This was a recurrent tumour in the proximity of the previously extirpated adenocarcinoma. Using specific PCR primers for conserved regions of the mouse mammary tumour virus (MMTV) no endogenous provirus DNA could be detected in DNA samples isolated from formalin-fixed paraffin embedded tissue sections.Keywords: hamsters; neoplasias; atypical fibrosarcoma; mammary gland; clinic; pathology; molecular biology Hamsters are popular pet animals with Syrian hamsters (Mesocricetus auratus) and Djungarian hamsters (Phodopus sungaros) being among the most frequently kept species. Reports on spontaneous tumours in domestic hamsters are scarce, and most are individual case reports (Kondo et al. 2008a). The incidence of tumours in these two species is quite different. In the analysis of tumours from 85 domestic hamsters 70 were Djungarian hamsters and 15 Syrian hamsters (Kondo et al. 2008a). In the integumentary system of Syrian hamsters melanomas and cutaneous lymphoma are the most frequently detected tumours, but both are low in incidence (Percy and Barthold 1993;Brown and Donnelly 2012). Tumours of the mammary gland in Syrian hamsters are rare and only a few cases have been described in the literature (e.g. Jelinek 1986a). However, in Djungarian hamsters the incidence of cutaneous neoplasias is five times greater than in Syrian hamsters and these are predominantly represented by mammary tumours, atypical fibromas and papilomas (Brown and Donnelly 2012). Kondo el al. (2009) analysed mammary tumours from 12 domestic Djungarian hamsters. Eleven were female and one was male. Histopathology revealed three subtypes: simple adenoma, tubulopapillary carcinoma, and complex carcinoma. No angioinvasion was observed. It is the aim of this contribution to describe mammary neoplasias in a female of Djungarian hamster and her two daughters. Case descriptionClinical and gross pathology. The owners of the pets in question originally intended to acquire two female Djungarian hamsters as pets. However, in the pet shop they purchased, by mistake, one male and one female. Two months later the female gave birth to four young. After weaning, three young animals (two females and one male) and the father were sold back to the pet shop and the family retained the mother and her daughter. The mother was, however, fertilized before the weaning of offspring and one month after the first parturition, bore a second litter of young comprised of two hamsters; one died shortly after birth while the other, female, survived.A tumour of the first left mammary gland was observed in the mother at the age of 15 months. At the time of extirpation its size was 2 × 1 × 1 cm

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mentioning

confidence: 88%

Familial incidence of mammary gland tumours in the Djungarian hamster (Phodopus sungaros): a case report

Jelínek¹,

Felsberg²,

Měšťan³

2013

Vet. Med. - Czech

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Ultrastructural studies on the replication and morphogenesis of Nairobi sheep disease virus, aNairovirus

Rwambo

Shaw²,

Rurangirwa

et al. 1996

Archives of Virology

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The Nairovirus Nairobi sheep disease virus (NSDV) affects sheep and goats causing severe hemorrhagic gastroenteritis and high mortality. Replication and morphogenesis of NSDV was determined by electron microscopic examination of ultra-thin sections of 143B and BHK-21 cells at varying times after infection. By 4 h post-infection (p.i.) of 143B cells, virions budding from the luminal side of the bilayer membrane of smooth membrane vesicles were observed. Morphologically mature virus particles were electron-dense, spherical and of uniform size (100 nm diameter) and accumulated in smooth membrane vesicles associated with the Golgi complex. In BHK-21 clone 13 cells, mature virus particles in smooth membrane vesicles were present by 8 h p.i. The morphogenesis of NSDV was restricted to the smooth membrane vesicles of Golgi complex, and budding of virus from other sites was not detected. Extracellular virus particles were observed by 10 h p.i., before expression of cytopathic effects. The cytopathic effects were observed at 24 h p.i. in 143B cells and at 36 h p.i. in BHK-21 cells. The morphology and morphogenesis of NSDV in BHK-21 cells and in 143B cells resembles that of other members of the family Bunyaviridae.

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Biological and molecular studies on Syrian hamster intracisternal R-type particles

Cited by 2 publications

References 27 publications

Familial incidence of mammary gland tumours in the Djungarian hamster (Phodopus sungaros): a case report

Familial incidence of mammary gland tumours in the Djungarian hamster (Phodopus sungaros): a case report

Ultrastructural studies on the replication and morphogenesis of Nairobi sheep disease virus, aNairovirus

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