Biological and Molecular Detection of Toxic Lipodepsipeptide-Producing
            <i>Pseudomonas syringae</i>
            Strains and PCR Identification in Plants

Bultreys, Alain; Gheysen, Isabelle

doi:10.1128/aem.65.5.1904-1909.1999

Cited by 72 publications

(52 citation statements)

References 50 publications

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“…For molecular detection of LP producers, PCR-based methods were developed for syringomycin-producing P. syringae (Quigley & Gross, 1994;Sørensen et al, 1998;Bultreys & Gheysen, 1999) and for tolaasin-producing Pseudomonas tolaasii (Lee et al, 2002). For Bacillus, PCR primers were developed against specific sequences in various LP biosynthesis genes (sfp, srf, fenD, ituC, bmyB) and successfully used to identify soil isolates with antifungal activity (Giacomodonato et al, 2001;Hsieh et al, 2004;Joshi & McSpadden Gardener, 2006;Mohammadipour et al, 2009).…”

Section: Detection and Identification Of Lpsmentioning

confidence: 99%

Natural functions of lipopeptides fromBacillusandPseudomonas: more than surfactants and antibiotics

et al. 2010

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Lipopeptides constitute a structurally diverse group of metabolites produced by various bacterial and fungal genera. In the past decades, research on lipopeptides has been fueled by their antimicrobial, antitumour, immunosuppressant and surfactant activities. However, the natural functions of lipopeptides in the lifestyles of the producing microorganisms have received considerably less attention. The substantial structural diversity of lipopeptides suggests that these metabolites have different natural roles, some of which may be unique to the biology of the producing organism. This review gives a detailed overview of the versatile functions of lipopeptides in the biology of Pseudomonas and Bacillus species, and highlights their role in competitive interactions with coexisting organisms, including bacteria, fungi, oomycetes, protozoa, nematodes and plants. Their functions in cell motility, leading to colonization of novel habitats, and in the formation and development of highly structured biofilms are discussed in detail. Finally, this review provides an update on lipopeptide detection and discovery as well as on novel regulatory mechanisms and genes involved in lipopeptide biosynthesis in these two bacterial genera.

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Section: Detection and Identification Of Lpsmentioning

confidence: 99%

Natural functions of lipopeptides fromBacillusandPseudomonas: more than surfactants and antibiotics

et al. 2010

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“…The production of syringomycin was determined by measurement of the diameters of the inhibition zones surrounding P. syringae colonies. To detect whether bacterial isolates obtained in this study possess the syrB and/or syrD genes responsible for the production of syringomycin, two specific primer pairs were used (Sorensen et al, 1998;Bultreys & Gheysen, 1999; Table 2). Presence or absence of a product of the expected size was taken as indication that the isolate studied either possessed or did not possess genes involved in synthesis of syringomycin.…”

Section: Syringomycin Production On Agar Medium and Detection Of Syrbmentioning

confidence: 99%

Phenotypic and genetic characterization of Pseudomonas syringae strains associated with the recent citrus bacterial blast and bacterial black pit epidemics in Tunisia

Abdellatif¹,

Kałużna

Janse

et al. 2017

Plant Pathology

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In the spring of 2012, symptoms of a disease resembling citrus blast and citrus black pit were observed in some orchards in Tunisia. The epidemic spread rapidly in the following years. Twenty‐four commercial citrus orchards from four Tunisian regions showing characteristic symptoms of bacterial diseases were surveyed during a 3‐year study. Eighty‐eight Pseudomonas‐like bacterial isolates were successfully obtained from the northeast and west of Tunisia. No isolates were recovered from the central region. Overall, 46 isolates were identified as Pseudomonas syringae pv. syringae and most of them showed similar phenotypic and genetic profiles. The virulence of three selected isolates differed from one plant cultivar to another as well as from the type of plant organ used for the inoculation. In a bioassay test, all isolates produced syringomycin, which was confirmed by molecular detection based on the syrB and syrD genes. Only EC122 possessed syrD but not syrB. DNA fingerprints, based on repetitive sequence‐based polymerase chain reaction (rep‐PCR) and PCR melting profile (PCR MP), were used to determine the potential genetic diversity among strains. Clustering of PCR MP fingerprinting data matched with rep‐PCR fingerprinting data. The generated distribution tree showed that Tunisian isolates were closely related to the citrus reference strain LMG5496. In contrast, EC112, isolated from citrus, and the almond isolate EC122 were distantly related to the type strain LMG1247T isolated from lilac. Such studies have not been reported until now for P. syringae from citrus.

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“…In addition, to further discriminate the Psa populations, the duplex-PCR technique of Gallelli et al (2011) was applied to representative Psa strains of the four populations. The putative presence of the syrB gene coding for the phytotoxin syringomycin was detected as previously described (Bultreys & Gheysen, 1999;Scortichini et al, 2003).…”

Section: Colony Morphology and Gatta Testsmentioning

confidence: 99%

Redefining the global populations of Pseudomonas syringae pv. actinidiae based on pathogenic, molecular and phenotypic characteristics

Ferrante

Scortichini

2014

Plant Pathology

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Knowing the population structure of a pathogen is fundamental for developing reliable phytosanitary legislation, detection techniques, and control strategies based on the actual aggressiveness and distribution of the pathogen. Currently, four populations of Pseudomonas syringae pv. actinidiae (Psa) have been described: Psa 1, Psa 2, Psa 3 and Psa 4. However, diagnostic assays specific for Psa populations do not detect Psa 4, the less virulent (LV) strains isolated in New Zealand. Similarly, multilocus sequence typing (MLST) of housekeeping genes, or broad Psa strain genome comparisons, revealed that Psa 4‐LV strains clustered separately from other Psa populations. In order to examine whether the placement of Psa 4 in the pathovar actinidiae was appropriate, various tests were carried out. It was shown that the Psa 4‐LV strains induced leaf and shoot wilting in Prunus cerasus, extensive necrotic lesions in Capsicum annuum fruits, and no significant symptoms in Actinidia deliciosa. Moreover, repetitive‐sequence PCR fingerprinting, type III secretion system effector protein genes detection and colony morphology clearly indicated the distinctiveness of Psa 4‐LV strains from the other three Psa populations. Rep‐PCR molecular typing revealed a high similarity of the Psa 4‐LV strains with members of Pseudomonas avellanae species. The Psa 4‐LV strains, most probably, belong to a new, still unnamed pathovar. It was concluded that the Psa 4‐LV strains isolated in New Zealand do not belong to the pathovar actinidiae, and, consequently, three Psa populations pathogenic to Actinidia spp. should currently include Psa 1, Psa 2 and Psa 3.

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Biological and Molecular Detection of Toxic Lipodepsipeptide-Producing Pseudomonas syringae Strains and PCR Identification in Plants

Cited by 72 publications

References 50 publications

Natural functions of lipopeptides fromBacillusandPseudomonas: more than surfactants and antibiotics

Natural functions of lipopeptides fromBacillusandPseudomonas: more than surfactants and antibiotics

Phenotypic and genetic characterization of Pseudomonas syringae strains associated with the recent citrus bacterial blast and bacterial black pit epidemics in Tunisia

Redefining the global populations of Pseudomonas syringae pv. actinidiae based on pathogenic, molecular and phenotypic characteristics

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