Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu

Jakubowicz, Violeta; Taibo, Catalina; Sciocco-Cap, Alicia; Arneodo, Joel D.

doi:10.1016/j.jip.2019.107211

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…At this late stage of the disease progress, a widespread systemic infection was observed. In both cases, fat bodies, epidermis, and tracheal matrix were the most affected organs, as previously reported for RanuNPV (Jakubowicz et al 2019). Bright red polyhedra (cubic or tetrahedral, according to the virus species) were observed inside enlarged nuclei.…”

Section: Speed Of Kill Of Acmnpv and Ranunpv In R Nu Larvae Following...supporting

confidence: 83%

“…Before use, insects were kept in quarantine for two generations to assess their pathogenfree status. Insects were reared on artificial diet at 26 ± 2°C and a photoperiod of 14:10 (L:D) h (Jakubowicz et al 2019). Under these conditions, R. nu undergoes five larval instars (LI -LV), readily discriminated by their head-capsule widths (LI: 0.278 ± 0.007 mm, LII: 0.429 ± 0.022 mm, LIII: 0.723 ± 0.028 mm, LIV: 1.128 ± 0.035 mm, and LV: 1.692 ± 0.130 mm; n = 25 each).…”

Section: Insects Viruses and Inoculationmentioning

confidence: 99%

“…The original AcMNPV and RanuNPV isolates were obtained from two different groups of baculovirus-infected R. nu larvae collected in Santa Fe province (Argentina) in the 1990 decade. Both isolates have been characterized biologically and genetically (Rodríguez et al 2012, Jakubowicz et al 2019. Following multiplication in laboratory-reared larvae, the OBs of either virus were extracted using SDS, cheesecloth filtering and differential centrifugation, and quantified in a Neubauer chamber (Arneodo et al 2018).…”

Section: Insects Viruses and Inoculationmentioning

confidence: 99%

“…Previous works reported the natural infection of R. nu by generalist AcMNPV and specific, phylogenetically distant Rachiplusia nu nucleopolyhedrovirus (RanuNPV) (Rodríguez et al 2012, Jakubowicz et al 2019. Here, we have assessed the speed of kill of either virus, alone or combined, against R. nu larvae.…”

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confidence: 97%

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Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations

Decker-Franco

Taibo²,

Rienzo

et al. 2021

Journal of Economic Entomology

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The South American soybean pest, Rachiplusia nu (Guenée), is naturally infected by Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and Rachiplusia nu nucleopolyhedrovirus (RanuNPV). We compared their pathogenicity to fourth-instar R. nu larvae, by evaluating time to death and virus spread throughout the tissues in single and mixed infections. Bioassays showed that generalist AcMNPV had a faster speed of kill than specific RanuNPV, while the mixed-virus treatment did not statistically differ from AcMNPV alone. Histopathology evidenced similar tissue tropism for both viruses, but co-inoculation resulted in mostly AcMNPV-infected cells. In sequential inoculations, however, the first virus administered predominated over the second one. Implications on baculovirus interactions and biocontrol potential are discussed.

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Section: Speed Of Kill Of Acmnpv and Ranunpv In R Nu Larvae Following...supporting

confidence: 83%

Section: Insects Viruses and Inoculationmentioning

confidence: 99%

Section: Insects Viruses and Inoculationmentioning

confidence: 99%

mentioning

confidence: 97%

See 2 more Smart Citations

Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations

Decker-Franco

Taibo²,

Rienzo

et al. 2021

Journal of Economic Entomology

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“…Rachiplusia nu has been traditionally controlled through the use of chemical insecticides and Bt transgenic technology (if available) (Rimoldi et al, 2012; Russo et al, 2012; Murúa et al, 2018). In recent years, both environmental concerns and the need to prevent the emergence of resistant insect populations has driven research also on biological control alternatives (Pelizza et al, 2018; Jakubowicz et al, 2019; Decker‐Franco et al, 2021). In any case, conventional, organic, or integrated management practices require an in‐depth knowledge on species‐intrinsic factors influencing pest dynamics in the agroecosystem.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas

Decker‐Franco,

San Blas,

Balbi

et al. 2023

Entomologia Exp Applicata

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Rachiplusia nu (Guenée) (Lepidoptera: Noctuidae, Plusiinae) is a defoliator of soybean (Glycine max L., Fabaceae), sunflower (Helianthus annuus L., Asteraceae), alfalfa (Medicago sativa L., Fabaceae), and horticultural crops in temperate southern South America; however, there is little genetic information available about this pest insect. Recently, concerns about this insect have increased because of its suspected resistance to Bt soybean and its spread to tropical regions. To better understand pest dynamics, and therefore implement suitable management strategies, a thorough knowledge of the genetic variability and spatial structuring of populations is required. In this paper, R. nu larvae were sampled from various crops and locations in central Argentina (where the pest is endemic) to assess diversity and population structure through mtDNA sequence analysis. In addition to the analysis of the standard cytochrome c oxidase subunit I (COI) ‘barcode’ fragment, a new set of primers was developed to explore genetic variation in the cytochrome b (Cytb) gene. COI and Cytb concatenated sequences revealed an overall high haplotype diversity (mean ± SD = 0.798 ± 0.037) and low nucleotide diversity (0.00137 ± 0.00013). The haplotype network showed a total of 19 haplotypes, separated by at most five mutational steps. A main central haplotype and four additional frequent haplotypes (accounting for 80% of the specimens examined) were distributed across populations with no obvious pattern related to host plant or geographic origin. Analysis of molecular variance further supported a weak population structure, where genetic variation within populations was the main source of total variation (ca. 96%). Pairwise comparisons showed only minor genetic differentiation among some of the populations studied. Implications of these findings in the context of R. nu control are discussed.

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Loss of p24 from the Bombyx mori nucleopolyhedrovirus genome results in the formation of cuboidal occlusion bodies

Kokusho

Katsuma

2021

Virology

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Biological and molecular characterization of Rachiplusia nu single nucleopolyhedrovirus, a promising biocontrol agent against the South American soybean pest Rachiplusia nu

Cited by 6 publications

References 24 publications

Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations

Comparative Pathogenesis of Generalist AcMNPV and Specific RanuNPV in Larvae of Rachiplusia nu (Lepidoptera: Noctuidae) Following Single and Mixed Inoculations

Genetic diversity of the polyphagous pest Rachiplusia nu across crops and locations in the Argentine Pampas

Loss of p24 from the Bombyx mori nucleopolyhedrovirus genome results in the formation of cuboidal occlusion bodies

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