Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil

Almeida, A. M. R.; Sakai, Jun‐ichi; Hanada, Kaoru; Oliveira, Thalita G.; Belintani, Priscila; Kitajima, Elliot Watanabe; Souto, Eliezer Rodrigues de; Novaes, Tanara Garcia de; Nora, Paulo S.

doi:10.1590/s0100-41582005000400005

Cited by 20 publications

(13 citation statements)

References 23 publications

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“…In our study virus preparation has reacted with the antiserum specific to TSV, which was produced approximately 29 kDa protein in western blot assay. This results was supported by Almeida et al (2005) reported coat protein gene of TSV from infected soybean in Brazil had a molecular mass of 29.880 kDa. This suggested that necrosis disease of soybean was caused by the TSV.…”

Section: Discussionsupporting

confidence: 76%

“…Similarly, Bhat et al (2002) conducted serology and characterization of coat protein studies for the sunflower Ilarvirus from India and they reported that it should be regarded as a strain of TSV belonging to subgroup I, designated as TSV-SF, which shared 90% amino acid sequence identity with TSV (strain WC). Almeida et al (2005) amplified coat protein gene of TSV with a size of 717 nucleotides along with 287 nucleotides at 3' untranslated region using RT-PCR and the results revealed that nucleotides and amino acids showed 96 to 98% similarity to other TSV isolates. They also reported TSV isolate causing soybean bud blight disease in Brazil was reported to be a distinct strain of TSV (TSV-BR), which shared 81.3 and 80.7% nucleotide sequence homology with the CP gene of TSV-WC and TSV-MB (mungbean isolate from India).…”

Section: Discussionmentioning

confidence: 99%

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Molecular characterization and genetic diversity of Tobacco streak virus infecting soybean (Glycine max L.)

Rajamanickam¹,

Karthikeyan²

2016

Afr. J. Microbiol. Res.

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Soybean (Glycine max L.), the most popular oil seed crop, grown in the experimental fields of Tamil Nadu Agricultural University and adjoining areas exhibited symptoms of necrosis and stunting. The symptoms were similar to bud blight of soybean caused by the Tobacco streak virus (TSV) and the disease is most prevalent throughout the country. To study the nature of infection, sap inoculation of the soybean strain induced local as well systemic infection on cowpea plants cv. C 152 and resulted in the production of circular necrotic lesions and death of plants. The samples were also serologically positive in DAC-ELISA and it has also yielded a protein band of approximately 29 kDa corresponding to coat protein of TSV in Western blot assay. For the characterization of virus, RT-PCR was carried out with a newly designed coat protein gene specific primer, which resulted in amplification of the expected 929 bp size. Sequence analysis of the CP gene had nucleotide similarity of 80.6 to 99.3% with known isolates of TSV. The multiple sequence alignment revealed that CP gene showed single unique variation and some of deletion and addition mutation was found in nucleotide and amino acid sequences against the isolates of other soybean Brazil and USA isolates and produced single unique variation at position 344 where adenine was substituted with guanine. There was no deletion and addition between nucleotide sequences in the group of Indian isolates, further confirms the placement of the soybean isolate of TSV in a single subgroup.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Molecular characterization and genetic diversity of Tobacco streak virus infecting soybean (Glycine max L.)

Rajamanickam¹,

Karthikeyan²

2016

Afr. J. Microbiol. Res.

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“…Along with our previous record of TSV-parthenium transmission in parthenium seed (Sharman et al, 2009), and to the best of our knowledge, these are the first records of TSV seed transmission in Asteraceae species. While TSV-parthenium is genetically closely related to a Brazilian strain of TSV (Almeida et al, 2005;Sharman & Thomas, 2013), limited tests have been reported for the Brazilian strain and no seed transmission was found (Costa & Carvalho, 1961). Similar to our observations for TSV in parthenium and crownbeard, several other disease outbreaks caused by TSV or AgLV have also been linked to TSV-infected Asteraceae species.…”

Section: Discussionmentioning

confidence: 99%

“…TSV has been reported as the causal agent for major disease outbreaks in sunflower and mung bean in Australia (Sharman et al, 2008), in oilseed and pulse crops in India (Prasada Rao et al, 2000;Reddy et al, 2002) and in soybean in Brazil (Almeida et al, 2005) and the USA (Rabedeaux et al, 2005). In Australia and India, parthenium weed (Parthenium hysterophorus) is the major alternative host of TSV strains that are closely associated with disease Ann Appl Biol 167 (2015) 197-207 © 2015 The State of Queensland through the Department of Agriculture and Fisheries outbreaks in nearby crops (Prasada Rao et al, 2003;Sharman et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Natural host range, thrips and seed transmission of distinctTobacco streak virusstrains in Queensland, Australia

2015

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Diseases caused by Tobacco streak virus (TSV) have resulted in significant crop losses in sunflower and mung bean crops in Australia. Two genetically distinct strains from central Queensland, TSV-parthenium and TSV-crownbeard, have been previously described. They share only 81% total-genome nucleotide sequence identity and have distinct major alternative hosts, Parthenium hysterophorus (parthenium) and Verbesina encelioides (crownbeard). We developed and used strain-specific multiplex Polymerase chain reactions (PCRs) for the three RNA segments of TSV-parthenium and TSV-crownbeard to accurately characterise the strains naturally infecting 41 hosts species. Hosts included species from 11 plant families, including 12 species endemic to Australia. Results from field surveys and inoculation tests indicate that parthenium is a poor host of TSV-crownbeard. By contrast, crownbeard was both a natural host of, and experimentally infected by TSV-parthenium but this infection combination resulted in non-viable seed. These differences appear to be an effective biological barrier that largely restricts these two TSV strains to their respective major alternative hosts. TSV-crownbeard was seed transmitted from naturally infected crownbeard at a rate of between 5% and 50% and was closely associated with the geographical distribution of crownbeard in central Queensland. TSV-parthenium and TSV-crownbeard were also seed transmitted in experimentally infected ageratum (Ageratum houstonianum) at rates of up to 40% and 27%, respectively. The related subgroup 1 ilarvirus, Ageratum latent virus, was also seed transmitted at a rate of 18% in ageratum which is its major alternative host. Thrips species Frankliniella schultzei and Microcephalothrips abdominalis were commonly found in flowers of TSV-affected crops and nearby weed hosts. Both species readily transmitted TSV-parthenium and TSV-crownbeard. The results are discussed in terms of how two genetically and biologically distinct TSV strains have similar life cycle strategies in the same environment.

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“…Bud blight symptoms caused by TSV are similar to those caused by TRSV and PBNV. A TSV isolate causing soybean bud blight disease in Brazil (TSV-BR) was reported to be a distinct strain which shared 81.3% and 80.7% nucleotide sequence homology with the CP gene of TSV-WC and TSV-MV (mungbean isolate from India), respectively (Almeida, Sakai, et al, 2005). The TSV isolate that caused necrosis in Maharashtra, India, was characterized by analyzing CP gene sequences and designated as TSV-SB (Arun .…”

Section: Brazilian Bud Blightmentioning

confidence: 99%

Tropical Food Legumes

Hema

Sreenivasulu

Patil

et al. 2014

Control of Plant Virus Diseases - Seed-Propagated Crops

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Biological and molecular characterization of an isolate of Tobacco streak virus obtained from soybeans in Brazil

Cited by 20 publications

References 23 publications

Molecular characterization and genetic diversity of Tobacco streak virus infecting soybean (Glycine max L.)

Molecular characterization and genetic diversity of Tobacco streak virus infecting soybean (Glycine max L.)

Natural host range, thrips and seed transmission of distinctTobacco streak virusstrains in Queensland, Australia

Tropical Food Legumes

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