Biological and molecular characterization of Uruguayan citrus tristeza virus field isolates

Rubio, Leticia Fernández; Bertalmío, Ana; Hernández-Rodríguez, Lester; Galeano, María José Benítez; Arruabarrena, Ana; Rivas, Fernando; Colina, Rodney; Maeso, Diego

doi:10.1007/s42161-018-0149-0

Cited by 6 publications

(9 citation statements)

References 25 publications

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“…Direct sequencing of the p20, p23 and p25 genes from the CTV isolates and their SAT derived sub-isolates used in this study showed a high inter-and intra-group genetic diversity, in some cases closer to the comparisons among reference sequences of the seven CTV strains. Phylogenetic analyses showed the presence of the extant strains VT, T3, NC, and RB in the majority of the genotype composition of the isolates and derived sub-isolates, as previously described (Benítez-Galeano et al 2015;Rubio et al 2019).…”

Section: Discussionsupporting

confidence: 69%

“…CTV isolates in field trees are often composed of variable populations of multiple strains that may enhance changes in the population genetic diversity, leading to the generation of new viral variants due to recombination events, or complementation among different strains (Brlansky et al 2003;Folimonova 2013;Harper et al 2015b). In Uruguay, CTV severe SP isolates are widespread (Benítez-Galeano et al 2015;Rubio et al 2019) and a high genetic diversity in field populations has been reported (Benítez-Galeano et al 2015). The study of CTV strain circulation could provide important information to understand disease development and implement management strategies in the country.…”

Section: Discussionmentioning

confidence: 99%

“…Finding mild protecting CTV isolates with homologous genotypes to the severe ones that need to be controlled is an important challenge to accomplish in a longterm CTV cross-protection system (Folimonova 2013). A first attempt to identify mild CTV isolates from a collection of Uruguayan field isolates failed due to the high intensity of the symptoms induced (Rubio et al 2019). In our study, SAT allowed us to segregate TR7-3, TR7-18, TR7-23, SACH-7, SACH-9, I58-7, I58-27 and DSST-9 sub-isolates, which resulted in MCA13 non-reactive, mild CTV isolates.…”

Section: Discussionmentioning

confidence: 99%

“…The TR7 isolate was collected in 1990 from an asymptomatic tree in a commercial 'Criollo' lemon (C. limon) orchard at the Southern region of Uruguay. All these field isolates (hereinafter "CTV isolates") were recently characterized by Rubio et al (2019). The isolates were grafted on 'Mexican' lime (ML) [C. aurantifolia (Christm.)…”

Section: Ctv Field Isolatesmentioning

confidence: 99%

“…CTV and its vector T. citricida have an endemic distribution in the country (Koch de Brotos and Boasso 1955). Recent studies revealed the presence of VT, T3, T36, RB and NC strains, as well as complex genetic mixtures of CTV populations (Benítez-Galeano et al 2015;Hernández-Rodríguez et al 2017) and the spread of severe SP-causing isolates (Rubio et al 2019).…”

Section: Introductionmentioning

confidence: 99%

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Diversity of Uruguayan citrus tristeza virus populations segregated after single aphid transmission

Hernández-Rodríguez

Benítez-Galeano

Bertalmío

et al. 2019

Trop. plant pathol.

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Prevalence of citrus tristeza virus (CTV) isolates causing severe stem pitting symptoms with a high diverse genetic composition represents a threat for citrus crops in Uruguay. This work aimed to characterize viral components of CTV populations segregated by single aphid (Toxoptera citricida) transmission (SAT). Thirty-nine SAT-derived sub-isolates were obtained from six CTV field isolates. Only eight were MCA13 non-reactive, and six of them, together with other three MCA13 reactive sub-isolates, were considered mild by the intensity of their reaction in 'Mexican' lime, 'Madam Vinous' sweet orange, 'Duncan' grapefruit and sour orange indicator plants. Fourteen sub-isolates, including two MCA13 non-reactive, were considered moderate, and the remaining sub-isolates were all MCA13 reactive and with severe reactions in the indicator plants. Sequence comparisons of the p20, p23 and p25 genes showed a high inter (among all isolates and their derived sub-isolates)-and intra (among each isolate and its derived sub-isolates)-specific evolutionary diversity, with the presence of the CTV strains VT, T3, RB, and NC in mixtures of their complex populations. Together, our data revealed the heterogeneity of the Uruguayan CTV populations in these CTV field isolates. The results provide additional biological and molecular information on the complex CTV populations in Uruguay and must be considered for improving the CTV management program.

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Section: Discussionsupporting

confidence: 69%