Biological and Molecular Characterization of a Crucifer Tobamovirus Infecting Oilseed Rape

Abstract

In China, the tobamovirus that infects oilseed rape has been misdiagnosed as Tobacco mosaic virus (TMV) based on its morphological similarity and serological relatedness. Recently, a tobamovirus has been isolated from oilseed rape in China, which we named Youcai mosaic virus Br (YoMV-Br), according to its biological and molecular characteristics. It had strong infectivity to Cruciferae but less to Solanaceae, Leguminosae, and Cucurbitaceae, and its virion morphology was consistent with that of the tobamoviruses. At high concentrations, it serologically cross reacted with TMV antiserum. The 3' terminal sequence (2,283 nucleotides) of YoMV-Br was determined, including the 3' noncoding region, the CP and MP genes, and the C-terminal part of the replicase gene. Between the MP and CP genes, 77 nucleotides overlapped. Compared with homologous regions of 21 recognized species of Tobamovirus, YoMV-Br had a much higher identity to crucifer species than to other tobamoviruses. Phylogenetic analysis demonstrated that YoMV-Br was closely related to the YoMV cluster of tobamoviruses and distantly to TMV, so that they likely belong to different strains of the same species.

Effective tolerance based on resource reallocation is a virus‐specific defence in Arabidopsis thaliana

“…More significant, four of these virus species, CMV, TuMV, TCV and CaMV, have been found to infect field populations of Arabidopsis, with incidences of up to 70% according to year and host population site (Pag an et al, 2010), indicating that their interaction with Arabidopsis is significant in nature, and could have resulted in the evolution of host defences. YoMV has also been demonstrated to infect several wild species in experimental conditions (Cai et al, 2009), including Arabidopsis (Aguilar et al, 1996), and has also been detected in wild plant populations (Park et al, 2016).…”

Characterization of the complete genome of ribgrass mosaic virus isolated from Plantago major L. from New Zealand and Actinidia spp. from China

“…This has created uncertainty in the naming of the previously published RMV sequences of isolates from Brassicaceae and Balsaminaceae [1]. This was also recognised by Cai et al [6], who commented that ''in some cases the unclear elevation of isolates to species has resulted in confusing nomenclature''. For example, two isolates originally designated RMV by Sheng et al [20] and Zhu et al [25] have subsequently been shown to be most similar to YoMV.…”

Aphids on Aphid-Susceptible Cultivars Have Easy Access to Turnip Mosaic Virus, and Effective Inoculation on Aphid-Resistant Cultivars of Oilseed Rape (Brassica napus)

“…As a non-persistently and non-circulatively transmitted virus, TuMV can infect the oilseed rape Brassica napus L. [21,22], resulting in a yield reduction of up to 70% [22].…”

“…TuMV was found in more than 24% of the cases of oilseed rape diseases in ten different regions of China, resulting in a 50 to 80% yield loss in oilseed rape [21]. The main TuMV vectors in significant rapeseed-producing regions along the lower reaches of the Yangtze River in China were Lipaphis erysimi Kaltenbach and M. persicae, but Brevicoryne brassicae L. (Hemiptera: Aphididae) population has steadily grown and received more attention lately than the other two species [23,24].…”