Biological and chemical properties of the hemoregulatory peptide and possibilities for clinical applications

Lærum, Ole Didrik; Paukovits, Walter R.

doi:10.1016/0163-7258(89)90007-7

Cited by 6 publications

(1 citation statement)

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“…Its structure was first reported in 1982 by Paukovits and Laerum [l]. While the peptide was a potent inhibitor of myelopoiesis with its main effect on the myeloid progenitor cells (CFU-GM) [2], it turned out that the dimer of the compound was a strong stimulator of the same cells [3,4]. In vivo, the myeloid precursor cells as well as CFU-GM and multipotent stem cells were stimulated [3], although very little effect was seen on the granulocyte numbers in peripheral blood.…”

Section: Introductionmentioning

confidence: 99%

Hemoregulatory peptide (HP5b) dimer effects on normal and malignant cells in culture

et al. 1993

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The dimer of the hemoregulatory peptide HP5b has been investigated for biological effects on various cell types in culture including mouse granulocyte-macrophage colony forming units (CFU-GM) from agar and murine long-term bone marrow culture (LTBMC). While CFU-GM were significantly stimulated in both systems, mitogen activation of mouse T, B and natural killer (NK) cells was not affected. Peptide treated mouse 3T3 fibroblasts reached a higher saturation density than controls; otherwise no effect was seen. A series of malignant cell lines was also tested. On a human glioblastoma cell line (GaMg) and rat glioma cell line (BT5C) a slight but significant stimulatory effect was found, while human mammary carcinoma cells (MCF7) were not affected. On SC1 mouse lymphoma cells a slight stimulation of cell growth was seen during the first part of exponential growth. Since HP5b acts as a stimulator for stromal cell secretion of other growth factors, supernatants from a human bone marrow stromal cell line stimulated with HP5b were tested on various cell lines. The effects of the supernatants on cell growth of the tested cell lines were not affected by HP5b treatment. Taken together with available in vivo data, the results indicate that the hemoregulatory peptide is a selective stimulator of myelopoiesis.

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Section: Introductionmentioning

confidence: 99%