Biological and chemical investigation of Allium cepa L. response to selenium inorganic compounds

Michalska-Kacymirow, Magdalena; Kurek, Eliza; Smolis, A.; Wierzbicka, Małgorzata; Bulska, Ewa

doi:10.1007/s00216-014-7742-7

Cited by 33 publications

(19 citation statements)

References 8 publications

(13 reference statements)

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“…According to the International Union of Pure and Applied Chemistry (IUPAC) statement [32], selection of a suitable extraction method for individual compounds is one of the most difficult tasks in speciation analysis, mainly due to their very low concentration and presence in complex matrices. The extraction procedures of selenium species from biological samples involve boiling in water [33][34][35], use of Tris-HCl buffer, acid hydrolysis [36] and enzymes such as protease [36], pronase [37], proteinase K [38], and protease-lipase [20]. Extraction is commonly supported by sonication or mechanical shaking of the sample with added inorganic and organic solvents.…”

Section: Introductionmentioning

confidence: 99%

Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods

Gawor

Ruszczyńska

Czauderna

et al. 2020

Animals

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Identification and quantification of the selenium species in biological tissues is imperative, considering the need to properly understand its metabolism and its importance in various field of sciences, especially nutrition science. Although a number of studies deals with the speciation of selenium, speciation analysis is still far from being a routine task, and so far strongly depends on the type of the samples. We present a study aimed to examine speciation analysis of Se in tissues of livers, muscles, and hearts obtained from lambs, namely in liver, muscle, and heart. The studied lambs were fed with the diet enriched with an inorganic (as sodium selenate) and organic chemical form of Se (as Se-enriched yeast) compounds with simultaneous addition of fish oil (FO) and carnosic acid (CA). The first part of the work was focused on the optimization of the extraction procedure of selenium compounds from tissues. Next, hyphenated high performance liquid chromatography and inductively coupled plasma mass spectrometry (HPLC–ICP–MS) was used for the identification of five seleno-compounds—Se-methionine (SeMet), Se-cystine (SeCys2), Se-methyl-Se-cysteine (SeMetSeCys), and Se(IV) and Se(VI). Verification of the identified seleno-compounds was achieved using triple-quadrupole mass spectrometer coupled to high performance liquid chromatography (HPLC–ESI–MS/MS). The applied procedure allowed for quantitative analysis of SeMet, SeCys2, and SeMetSeCys, in biological tissues. The developed analytical protocol is feasible for speciation analysis of small molecular seleno-compounds in animals samples.

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Section: Introductionmentioning

confidence: 99%

Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods

Gawor

Ruszczyńska

Czauderna

et al. 2020

Animals

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“…Se, an essential trace element, is involved in various physiological processes within our bodies and protects against several diseases, including Alzheimer's disease, cardiovascular disease and cancer . Several studies have demonstrated that a number of daily foods and nutritional supplements, e.g., garlic, onion, yeast and chlorella extracts, contain large amounts of Se . In 1999, a study by Ganther demonstrated the biological activity of Se was provided by Se‐conjugate compounds, not the element itself .…”

Section: Introductionmentioning

confidence: 99%

Se-Allylselenocysteine induces autophagy by modulating the AMPK/mTOR signaling pathway and epigenetic regulation of PCDH17 in human colorectal adenocarcinoma cells

Wang

Tsai

et al. 2015

Mol. Nutr. Food Res.

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“…Anion-exchange chromatography has been mainly employed in selenium speciation analysis [58][59][60][61][62][63]. Mobile phases used in this mode usually contain small content of organic modifier (e.g., 2-5% methanol) and buffered salt solution (e.g., acetate, phosphate, and citrate).…”

Section: Ion-exchange Chromatographymentioning

confidence: 99%

Chromatographic Analysis of Selenium Species

Sentkowska¹

2020

Importance of Selenium in the Environment and Human Health

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Selenium is an important element in environmental and living organisms that is being essential in very narrow concentration range, while deficiency or toxicity occurs outside this range. However, its toxicity depends not only on its dose but also on its chemical form. In environmental samples, selenium can exist in inorganic forms (as elemental selenium, metal selenides, selenite, or selenate anions) and as organic species with direct C-Se bonds (methylated compounds, selenoaminoacids, and selenoproteins). Thus, the development of reliable techniques to study the speciation of selenium in environmental samples is necessary. The main purpose of this chapter is to provide an update on the recent literature concerning the strategies for selenium speciation in environmental samples. Liquid chromatography coupled with sensitive detector is a commonly used technique for selenium separation. Gas chromatography can also be applied for such purpose; however derivatization step is usually required before analysis. Direct determination of selenium species at the concentration levels present in natural samples is very often difficult or even impossible. For this, several preconcentration/separation procedures for selenium have been proposed, including coprecipitation, extraction into an organic solvent, or application of solid sorbents.

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Biological and chemical investigation of Allium cepa L. response to selenium inorganic compounds

Cited by 33 publications

References 8 publications

Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods

Determination of Selenium Species in Muscle, Heart, and Liver Tissues of Lambs Using Mass Spectrometry Methods

Se-Allylselenocysteine induces autophagy by modulating the AMPK/mTOR signaling pathway and epigenetic regulation of PCDH17 in human colorectal adenocarcinoma cells

Chromatographic Analysis of Selenium Species

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