Biological and chemical characterization of new isolated halophilic microorganisms from saltern ponds of Trapani, Sicily

Villanova, Valeria; Galasso, Christian; Fiorini, Federica; Lima, Serena; Brönstrup, Mark; Sansone, Clementina; Brunet, Christophe; Brucato, Alberto; Scargiali, Francesca

doi:10.1016/j.algal.2021.102192

Cited by 12 publications

(8 citation statements)

References 41 publications

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“…is described for the use in a biological RPB treatment process. The discovery and usage of novel isolated halophilic microorganisms and their exploitation in a biotechnological and industrial context was already successfully demonstrated ( Guven et al, 2018 ; Rezaei Somee et al, 2018 ; Zare et al, 2019 ; Hasanzadeh et al, 2020 ; Villanova et al, 2021 ). Besides, the usage of a co-culture might be even beneficial for the present bioprocess.…”

Section: Resultsmentioning

confidence: 99%

Reducing Organic Load From Industrial Residual Process Brine With a Novel Halophilic Mixed Culture: Scale-Up and Long-Term Piloting of an Integrated Bioprocess

Mainka

Herwig

Pflügl

2022

Front. Bioeng. Biotechnol.

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Integrating bioprocess solutions for treatment and subsequent reuse of saline residual process brine into industrial processes could increase the sustainability of production chains. However, such bioprocesses require large-scales and a robust operation over a prolonged period. Consequently, the aim of this study was to analyze scale-up equivalence as well as continuous and stable process performance of a previously established lab scale process for the degradation of organic contaminants (formate and aromatic compounds) in an industrial context. To that end, a pilot-scale bubble column bioreactor system equipped with a membrane-based cell retention system for process intensification was integrated at an industrial production site. The process was successfully scaled-up and continuously operated for more than 210 days. Overall, the process proved to be robust towards changing compositions of the residual process brine stream and degradation rates for organic contaminants were close to 100%. Interestingly, due to the unsterile process conditions, the original Haloferax mediterranei culture was replaced by a novel halophilic bacterial community consisting of three bacterial genera. To further improve process economics and productivity, an optimization of the co-substrate feeding strategy for glycerol is required, as results indicated a potential correlation between glycerol feeding and formate degradation rates. To that end, decoupling of the glycerol feeding from the residual process brine feed is a potential way to increase process control options and allow for easy adaptation of the process to changing residual process brine compositions. Ultimately, the process described here could be a promising alternative for chemical or physical methods of treating residual process brine and once more underlines the potential to exploit natural microbial diversity for industrial purposes.

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Section: Resultsmentioning

confidence: 99%

Reducing Organic Load From Industrial Residual Process Brine With a Novel Halophilic Mixed Culture: Scale-Up and Long-Term Piloting of an Integrated Bioprocess

Mainka

Herwig

Pflügl

2022

Front. Bioeng. Biotechnol.

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“…and Halomonas sp. have been isolated from saltern ponds of Trapani, Sicily [66], and Salinivibrio kushneri has been isolated from salterns, Spain [73]. Although many species belonging to the recovered genera have been described in previous studies, our findings spotlighted the diversity of these strains with emphasis on their extracellular enzymatic activity.…”

Section: Discussionmentioning

confidence: 73%

“…From saltern ponds in Trapani, Sicily, Halomonas sp., Salinibacter ruber, and Brevibacterium sp. have been isolated [66]. Various bacterial and archaeal halophiles were isolated from a hypersaline lake in the south of Tunisia, and identified as Salicola sp., Bacillus sp., Halorubrum sp., Natrinema sp., Haloterrigena sp.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting of Culturable Halophilic Bacteria Isolated from Mediterranean Solar Saltern for Extracellular Halotolerant Enzymes

Ali,

Abdel-Rahman,

Farahat

2024

Microbiol. Biotechnol. Lett.

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Halophilic bacteria are promising reservoirs for halotolerant enzymes that have gained much attention in biotechnological applications due to their remarkable activity and stability. In this study, 62 halophilic bacterial strains isolated from a solar saltern were screened for the production of various extracellular enzymes. The results revealed that 31 strains (50%) were positive for amylase production while 26 strains (41.9%) were positive for protease. Further, 22 strains (35.48%) exhibited β-glucosidase activity and only 17 (27.41%) demonstrated lipase activity. Of the investigated halophiles, ten strains growing in the presence of ≥15% NaCl (w/v) were selected and identified based on their 16S rRNA gene sequences as Halomonas meridiana, Salinivibrio costicola, Virgibacillus oceani, Virgibacillus marismortui, Marinobacter lipolyticus, Halobacillus karajensis, Salicola salis, Pseudoalteromonas shioyasakiensis, Salinicoccus amylolyticus, and Paracoccus salipaludis. Therefore, the present study highlights the diversity of the culturable halophilic bacteria in a Mediterranean solar saltern, harboring various valuable halotolerant enzymes.

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“…Other valuable carotenoids detected in Pt , Csp , and Ng were β-carotene and lutein/zeaxanthin, confirming previous results ( Serra et al., 2021 ; Villanova et al., 2022 ). It is known that β-carotene concentration content can increase in microalgae cultivated under salt stress ( Villanova et al., 2021a ). The salinity in the tested RAS wastewater was only slightly higher than in the microalgae cultivation medium ( Table 1 ).…”

Section: Discussionmentioning

confidence: 99%

Two-phase microalgae cultivation for RAS water remediation and high-value biomass production

et al. 2023

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The overall goal of this study was to provide solutions to innovative microalgae-based technology for wastewater remediation in a cold-water recirculating marine aquaculture system (RAS). This is based on the novel concept of integrated aquaculture systems in which fish nutrient-rich rearing water will be used for microalgae cultivation. The produced biomass can be used as fish feed, while the cleaned water can be reused, to create a highly eco-sustainable circular economy. Here, we tested three microalgae species Nannochloropis granulata (Ng), Phaeodactylum tricornutum (Pt), and Chlorella sp (Csp) for their ability to remove nitrogen and phosphate from the RAS wastewater and simultaneously produce high-value biomass, i.e., containing amino acids (AA), carotenoids, and polyunsaturated fatty acids (PUFAs). A high yield and value of biomass were achieved for all species in a two-phase cultivation strategy: i) a first phase using a medium optimized for best growth (f/2 14x, control); ii) a second “stress” phase using the RAS wastewater to enhance the production of high-value metabolites. Ng and Pt performed best in terms of biomass yield (i.e., 5-6 g of dry weight, DW.L-1) and efficient cleaning of the RAS wastewater from nitrite, nitrate, and phosphate (i.e., 100% removal). Csp produced about 3 g L-1 of DW and reduced efficiently only nitrate, and phosphate (i.e., about 76% and 100% removal, respectively). The biomass of all strains was rich in protein (30-40 % of DW) containing all the essential AA except Methionine. The biomass of all three species was also rich in PUFAs. Finally, all tested species are excellent sources of antioxidant carotenoids, including fucoxanthin (Pt), lutein (Ng and Csp) and β-carotene (Csp). All tested species in our novel two-phase cultivation strategy thus showed great potential to treat marine RAS wastewater and provide sustainable alternatives to animal and plant proteins with extra added values.

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Biological and chemical characterization of new isolated halophilic microorganisms from saltern ponds of Trapani, Sicily

Cited by 12 publications

References 41 publications

Reducing Organic Load From Industrial Residual Process Brine With a Novel Halophilic Mixed Culture: Scale-Up and Long-Term Piloting of an Integrated Bioprocess

Reducing Organic Load From Industrial Residual Process Brine With a Novel Halophilic Mixed Culture: Scale-Up and Long-Term Piloting of an Integrated Bioprocess

Bioprospecting of Culturable Halophilic Bacteria Isolated from Mediterranean Solar Saltern for Extracellular Halotolerant Enzymes

Two-phase microalgae cultivation for RAS water remediation and high-value biomass production

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