Biological and Bioelectrochemical Systems for Hydrogen Production and Carbon Fixation Using Purple Phototrophic Bacteria

Vasiliadou, Ioanna A.; Berná, Antonio; Manchon, Carlos; Melero, Juan A.; Martı́nez, Fernando; Esteve‐Núñez, Abraham; Puyol, Daniel

doi:10.3389/fenrg.2018.00107

Cited by 41 publications

(36 citation statements)

References 39 publications

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“…The application of photosynthetic biomaterials in electrochemical systems has generated increasing interest in the last decades, as they offer the possibility to convert light energy into electrical current with a sustainable and biofriendly approach . The harvested solar energy could be utilized for power generation, bio‐electrosynthesis, and photo‐biosensor development . Different biological entities have been investigated for this purpose, spanning from isolated cellular or subcellular photosynthetic apparatus to intact photosynthetic organisms, in which all these materials offer different advantages .…”

Section: Introductionmentioning

confidence: 99%

Purple Bacteria and 3D Redox Hydrogels for Bioinspired Photo‐bioelectrocatalysis

et al. 2019

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A major challenge for the implementation of intact bacterial cells in photo‐bioelectrochemical systems remains the hindered extracellular electron transfer. This study focuses on purple bacteria, photosynthetic microorganisms particularly interesting for the development of bioelectrochemical systems because of their versatile metabolisms. Although soluble monomeric redox mediators have been proven as effective systems for electron transfer mediation, their application in the field is not preferable owing to their toxicity and unwanted release into the environment. An abiotic/biotic photoanode is reported in which a bioinspired redox mediating system is implemented in a 3D geometry allowing to “electrically wire” intact bacterial cells. The 3D photoanode decreased the overpotential required for harvesting photoexcited electrons, operating at +0.073 V versus the saturated calomel electrode (SCE). Accordingly, the overpotential was significantly reduced compared with a pioneering Os‐redox polymer reported in literature, which required operation at +0.303 V versus SCE. These results provide the basis for further development of bio‐photoanodes for light‐powered biosensing and power generation.

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Section: Introductionmentioning

confidence: 99%

Purple Bacteria and 3D Redox Hydrogels for Bioinspired Photo‐bioelectrocatalysis

et al. 2019

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“…PPB growth on oxidized organic substrates, such as malic acid, produces CO 2 due to the substrates' oxidation, which then is be re-fixed into cell material using the Calvin cycle [11]. The electrons needed for this come from the transformation of glutamate during its metabolism, as has been previously reported [15]. Thereby, it can be concluded that an organic N source (N-glutamate) considerable decreases the CO 2 emissions as compared to an inorganic source (ammonium) in photo-fermentation processes focused on hydrogen production.…”

Section: Effect Of Nitrogen Sources On H 2 and Co 2 Productionmentioning

confidence: 74%

“…The mixed culture of purple phototrophic bacteria (PPB) was enriched from wastewater taken from a pilot-scale WWTP at Rey Juan Carlos University (Mostoles, Madrid, Spain). Enrichment process was performed following the procedure outlined by Vasiliadou et al [15]. Briefly, a 1 L suspended growth photo-bioreactor (PhBR) containing synthetic wastewater (SW) as growth medium was inoculated with 20% v/v of domestic wastewater.…”

Section: Enrichment Of Purple Phototrophic Bacteria (Ppb)mentioning

confidence: 99%

“…Highly reduced carbon sources relative to PPB biomass (e.g., butyrate), are expected to promote hydrogen production as compared to more-oxidized substrates (e.g., acetate and malate) [10]. However, it has been previously reported for both mixed and pure cultures of PPB, that H 2 yields are not strictly co-related with the carbon oxidation state [15][16][17]. Other factors affecting the H 2 yield from different organic substrates is the amount of organic carbon stored as polymers such as polyhydroxybutyrate (PHB), as well as the Calvin cycle flux competing for electrons [16].…”

Section: Introductionmentioning

confidence: 98%

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Optimization of H2 Production through Minimization of CO2 Emissions by Mixed Cultures of Purple Phototrophic Bacteria in Aqueous Samples

Vasiliadou

Melero

Molina

et al. 2020

Water

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One of the current challenges in the treatment of wastewater is the recovery and/or transformation of their resources into high value-added products, such as biohydrogen. The aim of the present study was to optimize the production of hydrogen by mixed cultures of purple phototrophic bacteria (PPB), targeting in low CO2 emission. Batch assays were conducted using different carbon (malic, butyric, acetic acid) and nitrogen (NH4Cl, Na-glutamate, N2 gas) sources by varying the chemical oxygen demand to nitrogen ratio (COD:N 100:3 to 100:44), under infrared radiation as sole energy source. Malate-glutamate (COD:N 100:5.5) and malate-NH4-N (COD:N 100:3) fed cultures, exhibited high H2 production rates of 2.3 and 2.5 mLH2/Lh, respectively. It was observed that the use of glutamate decreased the CO2 emission by 74% (13.4 mLCO2/L) as compared to NH4-N. The H2 production efficiency achieved by organic carbon substrates in combination with glutamate, was in the order of malic (370 mLH2/L) > butyric (145 mLH2/L) > acetic acid (95 mLH2/L). These substrates entailed partitioning of reducing power into biomass at 64%, 50% and 48%, respectively, whereas reductants were derived to biohydrogen at 5.8%, 6.1% and 2.1%, respectively. These results suggest that nitrogen source and carbon dioxide emissions play an important role in the optimization of hydrogen production by PPB.

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“…Employing extremophiles could extend the operational range of BES considerably, allowing operational temperature ranges beyond that of conventional photovoltaics. BES can be used for the remote storage/generation of energy [ 83 , 84 ], synthesis of biologically derived organic compounds [ [85] , [86] , [87] ], as self-powered biosensors [ 88 , 89 ] and for the treatment of wastewater and bioremediation applications [ [90] , [91] , [92] ].…”

Section: Resource Recovery Applications Of Anoxygenic Phototrophic Bamentioning

confidence: 99%

An overview of anoxygenic phototrophic bacteria and their applications in environmental biotechnology for sustainable Resource recovery

George

Vincent

Mackey

2020

Biotechnology Reports

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Highlights APB are phylogenetically and metabolically diverse, includes many extremophiles. Ability to harvest IR light and use of many inorganic electron donors useful. Electricity, polymers, fertilizers, feed, antioxidants and pigments recoverable. Purple non-sulfur bacteria well studied, have wide range of applications. Metabolic engineering key to improving productivity and metabolic traits.

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Biological and Bioelectrochemical Systems for Hydrogen Production and Carbon Fixation Using Purple Phototrophic Bacteria

Cited by 41 publications

References 39 publications

Purple Bacteria and 3D Redox Hydrogels for Bioinspired Photo‐bioelectrocatalysis

Purple Bacteria and 3D Redox Hydrogels for Bioinspired Photo‐bioelectrocatalysis

Optimization of H2 Production through Minimization of CO2 Emissions by Mixed Cultures of Purple Phototrophic Bacteria in Aqueous Samples

An overview of anoxygenic phototrophic bacteria and their applications in environmental biotechnology for sustainable Resource recovery

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