Biological and biochemical characterization of 4-META/ MMA-TBB resin

Ogawa, Takahiro

doi:10.15226/jdodt.2015.00147

Cited by 6 publications

(8 citation statements)

References 40 publications

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“…Several studies have shown that the cytotoxicity of a composite material depends on its monomer, photoinitiator, and inorganic-filler-particle components [ 22 , 47 ]. In any polymer-based materials, unreacted monomers have negative effects on various cells [ 10 , 12 , 13 , 16 , 17 , 28 , 33 , 48 , 49 , 50 ]. Bis-GMA and UDMA are the main components of composites, the former eluting at higher concentrations than the latter [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

“…The surface layer of composite material does not polymerize when in contact with oxygen, forming an unpolymerized layer due to its polymerization mechanism [ 13 , 24 , 33 , 35 ]. We therefore compared composite material with and without the unpolymerized surface layer.…”

Section: Discussionmentioning

confidence: 99%

“…In light of other potential methods to improve composite materials, N-acetyl cysteine, a precursor of glutathione, the most potent antioxidant in the body, effectively scavenges polymerization radicals and neutralizes chemicals that cause oxidative stress [ 9 , 10 , 15 , 17 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 , 70 , 71 ]. Tri-n-butyl borane, a polymerization initiator, also reduces cytotoxicity, due to the suppressive role of polymerization radicals [ 11 , 13 , 14 , 18 , 33 , 72 ]. These data provide design rules for the use of monomers and initiators to create novel composite materials with higher biocompatibility.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have reported cytotoxicity of monomer components, which varies with chemical composition, type, and amount of residual monomer leached [ 19 , 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 ]. In addition, the free radicals generated during and after polymerization cause significant cellular damage [ 11 , 13 , 14 , 15 , 32 , 33 ]. Even after photo-polymerization, bis-GMA is eluted as residual monomer for over a month [ 34 ].…”

Section: Introductionmentioning

confidence: 99%

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Conditional Mitigation of Dental-Composite Material-Induced Cytotoxicity by Increasing the Cure Time

Matsuura

Komatsu

Choi

et al. 2023

JFB

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Light-cured composite resins are widely used in dental restorations to fill cavities and fabricate temporary crowns. After curing, the residual monomer is a known to be cytotoxic, but increasing the curing time should improve biocompatibility. However, a biologically optimized cure time has not been determined through systematic experimentation. The objective of this study was to examine the behavior and function of human gingival fibroblasts cultured with flowable and bulk-fill composites cured for different periods of time, while considering the physical location of the cells with regard to the materials. Biological effects were separately evaluated for cells in direct contact with, and in close proximity to, the two composite materials. Curing time varied from the recommended 20 s to 40, 60, and 80 s. Pre-cured, milled-acrylic resin was used as a control. No cell survived and attached to or around the flowable composite, regardless of curing time. Some cells survived and attached close to (but not on) the bulk-fill composite, with survival increasing with a longer curing time, albeit to <20% of the numbers growing on milled acrylic even after 80 s of curing. A few cells (<5% of milled acrylic) survived and attached around the flowable composite after removal of the surface layer, but attachment was not cure-time dependent. Removing the surface layer increased cell survival and attachment around the bulk-fill composite after a 20-s cure, but survival was reduced after an 80-s cure. Dental-composite materials are lethal to contacting fibroblasts, regardless of curing time. However, longer curing times mitigated material cytotoxicity exclusively for bulk-fill composites when the cells were not in direct contact. Removing the surface layer slightly improved biocompatibility for cells in proximity to the materials, but not in proportion to cure time. In conclusion, mitigating the cytotoxicity of composite materials by increasing cure time is conditional on the physical location of cells, the type of material, and the finish of the surface layer. This study provides valuable information for clinical decision making and novel insights into the polymerization behavior of composite materials.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Conditional Mitigation of Dental-Composite Material-Induced Cytotoxicity by Increasing the Cure Time

Matsuura

Komatsu

Choi

et al. 2023

JFB

Self Cite

View full text Add to dashboard Cite

show abstract

“…In comparison, according to Nakagawa et al, testing 4-MET in luting materials containing resin showed biocompatibility in the cells present in dental pulp [ 49 , 50 ]. Additionally, it was reported that the lower pH values may result in higher toxicity of the dental adhesives [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

The Toxicity of Universal Dental Adhesives: An In Vitro Study

et al. 2021

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There is no consensus in the literature regarding the potential toxicity of universal dental adhesives (UDA). Being used in close proximity to the pulp, their biocompatibility should be an important factor in dental research. The aim of the present study was to evaluate the biocompatibility of UDA in an in vitro model. The study was performed using a monocyte/macrophage peripheral blood SC cell line (ATCC CRL-9855) on four specific UDA, namely: All-Bond Universal (Bisco); CLEARFIL Universal Bond Quick (Kuraray); G-Premio BOND (GC); Single Bond Universal (3M ESPE). The cytotoxicity of the investigated UDA was measured using the XTT colorimetric assay. The genotoxicity of the analyzed compounds was evaluated using an alkaline version of the comet assay. Furthermore, flow cytometry (FC) apoptosis detection was performed using the FITC Annexin V Apoptosis Detection Kit I. FC cell-cycle arrest assessment was performed using propidium iodide staining. The study observed significant differences in the toxicity of the UDA that were tested, as G-Premio BOND showed significant in vitro toxicity in all of the tests performed, while All-Bond Universal, CLEARFIL Universal Bond Quick and Single Bond Universal did not present any significant toxic effects toward SC cell line. The in vitro toxicity of UDA should be taken into consideration prior to in vivo and clinical studies. The flow cytometry could improve the accuracy of dental materials research and should be incorporated into the standardization criteria.

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Cell Type-Specific Effects of Implant Provisional Restoration Materials on the Growth and Function of Human Fibroblasts and Osteoblasts

et al. 2022

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Implant provisional restorations should ideally be nontoxic to the contacting and adjacent tissues, create anatomical and biophysiological stability, and establish a soft tissue seal through interactions between prosthesis, soft tissue, and alveolar bone. However, there is a lack of robust, systematic, and fundamental data to inform clinical decision making. Here we systematically explored the biocompatibility of fibroblasts and osteoblasts in direct contact with, or close proximity to, provisional restoration materials. Human gingival fibroblasts and osteoblasts were cultured on the “contact” effect and around the “proximity” effect with various provisional materials: bis-acrylic, composite, self-curing acrylic, and milled acrylic, with titanium alloy as a bioinert control. The number of fibroblasts and osteoblasts surviving and attaching to and around the materials varied considerably depending on the material, with milled acrylic the most biocompatible and similar to titanium alloy, followed by self-curing acrylic and little to no attachment on or around bis-acrylic and composite materials. Milled and self-curing acrylics similarly favored subsequent cellular proliferation and physiological functions such as collagen production in fibroblasts and alkaline phosphatase activity in osteoblasts. Neither fibroblasts nor osteoblasts showed a functional phenotype when cultured with bis-acrylic or composite. By calculating a biocompatibility index for each material, we established that fibroblasts were more resistant to the cytotoxicity induced by most materials in direct contact, however, the osteoblasts were more resistant when the materials were in close proximity. In conclusion, there was a wide variation in the cytotoxicity of implant provisional restoration materials ranging from lethal and tolerant to near inert, and this cytotoxicity may be received differently between the different cell types and depending on their physical interrelationships.

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Biological and biochemical characterization of 4-META/ MMA-TBB resin

Cited by 6 publications

References 40 publications

Conditional Mitigation of Dental-Composite Material-Induced Cytotoxicity by Increasing the Cure Time

Conditional Mitigation of Dental-Composite Material-Induced Cytotoxicity by Increasing the Cure Time

The Toxicity of Universal Dental Adhesives: An In Vitro Study

Cell Type-Specific Effects of Implant Provisional Restoration Materials on the Growth and Function of Human Fibroblasts and Osteoblasts

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