Biological analysis and imaging applications of CdSe/CdSxSe1−x/CdS core–shell magic-sized quantum dot

Silva, Anielle Christine Almeida; Freschi, Ana Paula Peres; Rodrigues, Claudia; Matias, Bruna França; Maia, Larissa Prado; Goulart, Luiz Ricardo; Dantas, Noélio O.

doi:10.1016/j.nano.2016.01.001

Cited by 29 publications

(21 citation statements)

References 42 publications

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“…In our opinion, it is very important to describe the way how the concentration of the nanomaterials was determined, so that the results described by different research groups could be compared. In some studies, the concentration of the nanoparticles is expressed as a number of particles per volume (Fratoddi et al 2015) or a mass of nanoparticles per volume (Silva et al 2016;Yan et al 2016). In this case, the solutions containing the same number of the nanoparticles may have various cadmium content.…”

Section: Conclusion and Discussionmentioning

confidence: 99%

Comparative studies of biological activity of cadmium-based quantum dots with different surface modifications

et al. 2018

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This paper presents a modification of the surface of CdS/ZnS and CdSe x S 1−x /ZnS quantum dots (QDs) with 3-mercaptopropionic and 6-mercaptohexanoic acid. The obtained QDs were characterized using TEM, DLS, UV-Vis, and fluorescence spectroscopy. Flow cytometry was applied to evaluate the cytotoxicity of QDs and examine the type of death caused by the tested nanoparticles. In addition, the generation of reactive oxygen species after incubation of the tested cells with CdSe x S 1−x / ZnS-MPA and CdSe x S 1−x /ZnS-MHA QDs was evaluated. The study was conducted on three cell lines: adherent (A549 and MRC-5) and suspension ones (K562). The conducted research demonstrated that the tested nanoparticles exhibit concentration-dependent toxicity. It was observed that the surface modification influences the toxicity level of the examined QDs, and modification of their surface with the use of the ligand of longer carbon chain (MHA) reduces the toxicity in comparison with QDs-MPA. It was also found that all tested QDs caused the death of cells in the course of necrosis. Based on obtained results, it was concluded that the cytotoxicity of QDs is to a large extent related to reactive oxygen species (ROS) generation.

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Section: Conclusion and Discussionmentioning

confidence: 99%

Comparative studies of biological activity of cadmium-based quantum dots with different surface modifications

et al. 2018

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“…In the biological labeling assays, we use CdSe/CdS MSQDs bioconjugated with Pepstatin A, BnSP-6, BthTx-II and a specific BC Fab antibody to allow its tracking within the cell and, in addition, to verify the specificity of this treatment for breast cancer cells. Figure 1a shows the absorption and emission spectra of CdSe/CdSe MSQDs that observed a narrow band around 428 nm and a broad emission spectrum, respectively [41]. This broad emission is characteristics of the MSQDs and that is of great applicability in biological processes, enabling their visualization in different detection channels, varying from blue to red (Figure 1a) [43].…”

Section: Introductionmentioning

confidence: 98%

“…The MSQDs can be important tools in the investigation of metastases and have been proposed as vehicles for administering drugs that can initiate certain photoactivated chemical reactions due to highly stable luminescence over time [39]. The cultures of cells marked with MSQDs continue to maintain their functions, remaining viable for analysis for prolonged periods of time [39][40][41][42].…”

Section: Introductionmentioning

confidence: 99%

Biocompatible Magic Sized Quantum Dots: Luminescent Markers and Probes

Silva¹,

Correia²,

Silva³

et al. 2018

State of the Art in Nano-Bioimaging

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Nanoscience and nanobiotechnology have aroused great academic and technological interest. Works relating biomaterials at the nanoscale can reach new biotechnologies and help in the development and use of tools for bioimage and diagnosis applications. In this work we demonstrated the advantages of magic sized quantum dots as luminescent markers and probes to bioimage applications. The visualization of MSQDs bioconjugated with biological probes in cells were performed at periods greater than 2 h, and visualization with no commercial dye would not be possible. Therefore, we demonstrated that theses biocompatible nanocrystals are luminescent markers and probes to diagnosis.

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“…[24][25][26][27][28][29][30] However, following surface modification of QDs, it is important to retain its fundamental properties, such as uniform dispersion and to ensure that functional groups remain available for biological applications. [31][32][33][34][35] As a result, development of targetable fluorescent probe QDs with fundamental properties of bio-molecules.…”

Section: Introductionmentioning

confidence: 99%

<p>A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells</p>

Zhang

Yao

Qiao

et al. 2020

IJN

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Purpose: Quantum dots (QDs) are used as fluorescent probes due to their high fluorescence intensity, longevity of fluorescence, strong light-resistant bleaching ability and high light stability. Therefore, we explore a more precise probe that can target an organelle. Methods: In the current study, a new class of fluorescence probes were developed using QDs capped with 4 different L-cysteine-polyamine-morpholine linked by mercapto groups. Ligands were characterised by Electrospray ionization mass spectrometry (ESI-MS), H-Nuclear Magnetic Resonance (1 H NMR) spectroscopy, and 13 C NMR spectroscopy. Modified QDs were characterized by Transmission Electron Microscope (TEM), Ultraviolet and visible spectrophotometry (UV-Vis), and fluorescence microscopy. And the biological activity of modified QDs was explored by using MTT assay with HeLa, SMMC-7721 and HepG2 cells. The fluorescence imaging of modified QDs was obtained by confocal laser scanning fluorescence microscopy (CLSM). Results: Synthesized QDs ranged between 4 to 5 nm and had strong optical emission properties. UV-Vis and fluorescence spectra demonstrated that the cysteine-polyaminemorpholine were successfully incorporated into QD nanoparticles. The MTT results demonstrated that modified QDs had lesser cytotoxicity when compared to unmodified QDs. In addition, modified QDs had strong fluorescence intensity in HeLa cells and targeted lysosomes of HeLa cells. Conclusion: This study demonstrates the modified QDs efficiently entered cells and could be used as a potential lysosome-targeting fluorescent probe.

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Biological analysis and imaging applications of CdSe/CdSxSe1−x/CdS core–shell magic-sized quantum dot

Cited by 29 publications

References 42 publications

Comparative studies of biological activity of cadmium-based quantum dots with different surface modifications

Comparative studies of biological activity of cadmium-based quantum dots with different surface modifications

Biocompatible Magic Sized Quantum Dots: Luminescent Markers and Probes

<p>A Lysosome-Targetable Fluorescence Probe Based on L-Cysteine-Polyamine-Morpholine-Modified Quantum Dots for Imaging in Living Cells</p>

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