Biological Aerosols

Grinshpun, Sergey A.

doi:10.1002/9783527630134.ch13

Cited by 13 publications

(14 citation statements)

References 129 publications

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“…Exposure to bioaerosols may result in a range of adverse health effects, including infectious disease, acute toxic effects, and allergy (3). The importance of bioaerosol research has in recent years been highlighted by several infectious disease outbreaks as well as the emerging bioterrorism threat capitalized by the 2001 Amerithrax incidents in the United States (2).…”

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confidence: 99%

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Aerobiological Stabilities of Different Species of Gram-Negative Bacteria, Including Well-Known Biothreat Simulants, in Single-Cell Particles and Cell Clusters of Different Compositions

Dybwad

Skogan

2017

Appl Environ Microbiol

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The ability to perform controlled experiments with bioaerosols is a fundamental enabler of many bioaerosol research disciplines. A practical alternative to using hazardous biothreat agents, e.g., for detection equipment development and testing, involves using appropriate model organisms (simulants). Several species of Gram-negative bacteria have been used or proposed as biothreat simulants. However, the appropriateness of different bacterial genera, species, and strains as simulants is still debated. Here, we report aerobiological stability characteristics of four species of Gram-negative bacteria (Pantoea agglomerans, Serratia marcescens, Escherichia coli, and Xanthomonas arboricola) in single-cell particles and cell clusters produced using four spray liquids (H 2 O, phosphate-buffered saline [PBS], spent culture medium [SCM], and a SCM-PBS mixture). E. coli showed higher stability in cell clusters from all spray liquids than the other species, but it showed similar or lower stability in single-cell particles. The overall stability was higher in cell clusters than in single-cell particles. The highest overall stability was observed for bioaerosols produced using SCM-containing spray liquids. A key finding was the observation that stability differences caused by particle size or compositional changes frequently followed species-specific patterns. The results highlight how even moderate changes to one experimental parameter, e.g., bacterial species, spray liquid, or particle size, can strongly affect the aerobiological stability of Gram-negative bacteria. Taken together, the results highlight the importance of careful and informed selection of Gram-negative bacterial biothreat simulants and also the accompanying particle size and composition. The outcome of this work contributes to improved selection of simulants, spray liquids, and particle size for use in bioaerosol research.IMPORTANCE The outcome of this work contributes to improved selection of simulants, spray liquids, and particle size for use in bioaerosol research. Taken together, the results highlight the importance of careful and informed selection of Gramnegative bacterial biothreat simulants and also the accompanying particle size and composition. The results highlight how even moderate changes to one experimental parameter, e.g., bacterial species, spray liquid, or particle size, can strongly affect the aerobiological stability of Gram-negative bacteria. A key finding was the observation that stability differences caused by particle size or compositional changes frequently followed species-specific patterns.

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confidence: 99%

“…Bioaerosol research is inherently multidisciplinary and demands scientists with an integrated skill set, including microbiology, molecular biology, occupational and public health, and aerosol physics (2). Exposure to bioaerosols may result in a range of adverse health effects, including infectious disease, acute toxic effects, and allergy (3).…”

mentioning

confidence: 99%

Aerobiological Stabilities of Different Species of Gram-Negative Bacteria, Including Well-Known Biothreat Simulants, in Single-Cell Particles and Cell Clusters of Different Compositions

Dybwad

Skogan

2017

Appl Environ Microbiol

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“…The period should be long enough to collect a representative sample of the airborne fungus [35]. However, selecting long sampling periods may lead to excessive desiccation and reduce the recovery capability of the sampling device [28,30,[35][36].…”

Section: Discussionmentioning

confidence: 99%

Assessment of interstitial mould growth through impaction: a feasibility study

2020

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The application of insulation to solid walls is one of the measures to help reduce the energy consumption of a building. Where external wall insulation is not feasible (e.g. listed buildings), internal insulation may be the only option to improve the thermal property of external walls. However, such interventions may be followed by unintended consequences [1], such as moisture accumulation and the growth of mould [2]. The study aims to develop a method for the non-disruptive assessment of interstitial mould growth in internally insulated walls. Air sampling through impaction and culture-based analysis was used in the study. Mould species commonly found in buildings were cultivated and used in small-scale experiments, and the effects of several sampling periods and airflow rates assessed. Potential relationships between the sampling variables and the results were determined by means of statistical analysis - a strong correlation between the coverage of mould behind the wall and the sampling periods and airflow rates. The effect of the inertial impaction frequency on the sampling results and the feasibility and applicability of the suggested methodology in real-life scenarios are also discussed.

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“…sedimentation) and air sampling through the creation of an artificial airflow (e.g. impaction, liquid impingement, filtration and electrostatic precipitation) [7][8][9]. It can be argued that in both cases, the two terms attempt to define whether samples are collected from still or mixed air, respectively.…”

Section: Passive (Non-activated) and Active (Activated) Sampling: Terminologymentioning

confidence: 99%

Air sampling and analysis of indoor fungi : a critical review of passive (non- activated) and active (activated) sampling

Efthymiopoulos¹,

Aktaş²,

Altamirano³

2021

Air Sampling and Analysis of Indoor Fungi : A Critical Review of Passive (Non- Activated) and Active (Activated) Sampling

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For over a century, researchers have been concerned with what testing protocols to assess indoor fungi growth were most reliable. To that end, various sampling and analysis methods were developed. However, to present no testing procedure has been standardised. The vast number of different fungi species, the differences in their biological properties and their implications on the occupants' health and building fabric can make the decision-making process for an appropriate assessment protocol challenging. This research aims to make a critical review of passive (or nonactivated) and active (or activated) sampling and emphasize potential errors while interpreting results obtained from passive or active protocols. Peer-review under the responsibility of the organizing committee of the ICMB21.

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Biological Aerosols

Cited by 13 publications

References 129 publications

Aerobiological Stabilities of Different Species of Gram-Negative Bacteria, Including Well-Known Biothreat Simulants, in Single-Cell Particles and Cell Clusters of Different Compositions

Aerobiological Stabilities of Different Species of Gram-Negative Bacteria, Including Well-Known Biothreat Simulants, in Single-Cell Particles and Cell Clusters of Different Compositions

Assessment of interstitial mould growth through impaction: a feasibility study

Air sampling and analysis of indoor fungi : a critical review of passive (non- activated) and active (activated) sampling

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Biological Aerosols

Cited by 13 publications

References 129 publications

Aerobiological Stabilities of Different Species of Gram-Negative Bacteria, Including Well-Known Biothreat Simulants, in Single-Cell Particles and Cell Clusters of Different Compositions

Aerobiological Stabilities of Different Species of Gram-Negative Bacteria, Including Well-Known Biothreat Simulants, in Single-Cell Particles and Cell Clusters of Different Compositions

Assessment of interstitial mould growth through impaction: a feasibility study

Air sampling and analysis of indoor fungi : a critical review of passive (non- activated) and active (activated) sampling

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Air sampling and analysis of indoor fungi : a critical review of passive (non- activated) and active (activated) sampling