Biological activity of SDS-CTAB cat-anionic vesicles in cultured cells and assessment of their cytotoxicity ending in apoptosis

Aiello, Cecilia; Andreozzi, Patrizia; Mesa, Camillo La; Risuleo, Gianfranco

doi:10.1016/j.colsurfb.2010.02.013

Cited by 51 publications

(35 citation statements)

References 30 publications

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“…Because the amphiphilic surfactants behaved like bi-tailed lipid molecule21, the mechanism between catanionic vesicles and cells includes two pathways, endocytosis and/or fusion2223. For example, the SDS-CTAB catanionic vesicles enter the cells via membrane fusion or endocytosis24. Azobenzene-containing surfactant/alkyl surfactant catanionic vesicles past the cell membrane through endocytosis25.…”

mentioning

confidence: 99%

A Light-Responsive Self-Assembly Formed by a Cationic Azobenzene Derivative and SDS as a Drug Delivery System

Geng

Wang

et al. 2017

Sci Rep

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The structure of a self-assembly formed from a cationic azobenzene derivative, 4-cholesterocarbonyl-4′-(N,N,N-triethylamine butyloxyl bromide) azobenzene (CAB) and surfactant sodium dodecyl sulfate (SDS) in aqueous solution was studied by cryo-TEM and synchrotron radiation small-angle X-ray scattering (SAXS). Both unilamellar and multilamellar vesicles could be observed. CAB in vesicles were capable to undergo reversible trans-to-cis isomerization upon UV or visible light irradiation. The structural change upon UV light irradiation could be catched by SAXS, which demonstrated that the interlamellar spacing of the cis-multilamellar vesicles increased by 0.2–0.3 nm. Based on this microstructural change, the release of rhodamine B (RhB) and doxorubicin (DOX) could be triggered by UV irradiation. When incubated NIH 3T3 cells and Bel 7402 cells with DOX-loaded CAB/SDS vesicles, UV irradiation induced DOX release decreased the viability of both cell lines significantly compared with the non-irradiated cells. The in vitro experiment indicated that CAB/SDS vesicles had high efficiency to deliver loaded molecules into cells. The in vivo experiment showed that CAB/SDS vesicles not only have high drug delivery efficiency into rat retinas, but also could maintain high drug concentration for a longer time. CAB/SDS catanionic vesicles may find potential applications as a smart drug delivery system for controlled release by light.

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confidence: 99%

A Light-Responsive Self-Assembly Formed by a Cationic Azobenzene Derivative and SDS as a Drug Delivery System

Geng

Wang

et al. 2017

Sci Rep

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“…The relationship between the extraction buffer and the concentration of organic and inorganic salts can affect the performance of the physical properties and the ability to separate the complexes formed by nucleic acids and surfactants agents (CTAB and SDS) in aqueous solution (Zhang and Teng, 2008). In addition, hyposaline concentrations in these buffers do not allow the precipitation of contaminants (polysaccharides, phenolic compounds, and proteins) from the complex of nucleic acids and can be removed by extracting the aqueous solution with an organic phase (Aiello et al 2010). On the other hand, brown colour and contamination (phenolic compounds) were reduced by adding of PVPP and high concentrations of β-mercaptoetanol in the extraction buffers.…”

Section: Resultsmentioning

confidence: 99%

Isolation of total RNA from tissues rich in polyphenols and polysaccharides of mangrove plants

Rubio-Piña¹,

Zapata-Pérez²

2011

Electro Journal of Biotech

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RNA extraction from mangrove tissues can be difficult due to the presence of polyphenolic and polysaccharide compounds upon cell disruption. Besides, a successful RNA isolation from mangrove tissues sometimes can be strain and species specific. Two different methods were used to extract RNA from tissues (stems, leaves and roots) of black mangrove (Avicennia germinans) and white mangrove (Laguncularia racemosa), collected from the mangrove area at Progreso, Yucatan, Mexico. An optimized and modified total RNA isolation method was developed for these recalcitrant species. The protocol is based on the CTAB method including β-mercaptoethanol and PVPP with sequential Phenol/Chloroform/isoamyl alcohol extractions to remove protein, and polyphenols, followed by two selective purifications with LiCl and sodium acetate to eliminate polysaccharides. Although, the introduced modifications are not new, their addition proved to be decisive for success in RNA isolation. This modified procedure resulted in high quantity and quality RNA. The RNA obtained is suitable for cDNA synthesis, RT-PCR experiments and the phytochelatin synthase 1 gene amplification.

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“…For further details about the experimental data treatment, see published work from our laboratory [35,[38][39][40].…”

Section: B Molecular Evaluation Of the Cell Damagementioning

confidence: 99%

Neurodegenerative and Morpho-functional Alterations During Senescence: Positive Modulation by Lipoic Acid and Superoxide Dismutase Ocular Senescence in a Rat Model

Scarsella

Risuleo

2015

LSMR

Self Cite

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Abstract-We have evaluated the modulation of degenerative events at the retinal and optic nerve level in a rat model system. Degeneration was ameliorated in aging rats by the combined oral administration of -lipoic acid and superoxide dismutase. Twenty four month-old rats were fed ad libitum with a diet supplemented by these two compounds. Positive control rats received a compound-free diet, while the negative control group consisted of untreated young animals. The cyto-protective effects of -lipoic acid and superoxide dismutase were investigated by morphological analysis of retinal and optic nerve head sections. The level of nuclear DNA damage was assessed by TUNEL fluorescent reaction. Lipo-peroxidation assays evaluated the oxidative stress and consequent alteration of the cell membrane function. Apoptotic phenomena were investigated by immuno-localization and Western blotting to measure the expression of caspase-3 and inducible nitric oxide synthase. The results reported in this work provide evidence that-lipoic acid and superoxide dismutase counteract the degenerative damage in aging animals, reducing the effects of oxidative stress.

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Biological activity of SDS-CTAB cat-anionic vesicles in cultured cells and assessment of their cytotoxicity ending in apoptosis

Cited by 51 publications

References 30 publications

A Light-Responsive Self-Assembly Formed by a Cationic Azobenzene Derivative and SDS as a Drug Delivery System

A Light-Responsive Self-Assembly Formed by a Cationic Azobenzene Derivative and SDS as a Drug Delivery System

Isolation of total RNA from tissues rich in polyphenols and polysaccharides of mangrove plants

Neurodegenerative and Morpho-functional Alterations During Senescence: Positive Modulation by Lipoic Acid and Superoxide Dismutase Ocular Senescence in a Rat Model

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