Biological activity of entomopathogenic actinomycetes against lepidopteran insects (Noctuidae: Lepidoptera)

VijayabharathiRajendran,; Ratna, KumariBhimineni; SathyaArumugam,; SrinivasVadlamudi,; AbhishekRathore,; SharmaHari, C; GopalakrishnanSubramaniam,

doi:10.4141/cjps2013-298

Cited by 35 publications

(23 citation statements)

References 39 publications

(28 reference statements)

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“…Biopesticides produced by microorganisms and used in agriculture have gained significant importance among researchers and the farming community because of their safety as regards non-target organisms and the environment. Moreover, biopesticides have reduced the emergence of natural and cross resistance in plant pests [29]. Actinobacteria, particularly Streptomyces spp., have provided a diverse range of secondary metabolites of high commercial significance and continue to be regularly screened for new bioactive compounds [30].…”

Section: Discussionmentioning

confidence: 99%

Insecticidal activity of endophytic actinomycetes isolated from Azadirachta indica against Myzus persicae

Chen¹,

Shafi²,

Mao-hai³

et al. 2018

Arch biol sci (Beogr)

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In the present study, 85 strains of actinomycetes were isolated from the neem tree (Azadirachta indica) and screened for their insecticidal activity against the green peach aphid, Myzus persicae. The results showed that crude extracts from 24 strains exerted a contact effect against M. persicae with different insecticidal efficacies. Crude extracts from 8 actinomycetes strains exhibited the highest insecticidal activity (above 60%). Out of these 8 strains, 3 isolates that produced the maximum mortalities were screened a second time. The crude extract from strain G30 was the most virulent against the green peach aphid, with LC 50 and LC 95 values of 1.680 mg/mL and 4.370 mg/mL, respectively, after 48 h of treatment. The following morphological, culture, physiological and biochemical characteristics of strain G30 were recorded: (i) ovateorbicular and smooth surface spores with short and curve filaments; (ii) an aerial off-white mycelium with a mustered yellow base; (iii) inability to produce soluble pigments; (iv) the ability to hydrolyze starch but not cellulose; (v) the ability to utilize glycerin and several sugars as a carbon source but not L-rhamnose and sorbitol. Molecular identification of G30 revealed a 99.6% genetic similarity of the 16S rDNA sequence with Streptomyces albidoflavus. We conclude that the isolate G30 was S. albidoflavus and that the insecticidal activity of its crude extract was sufficiently high to become a candidate for bioinsecticide development.

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Section: Discussionmentioning

confidence: 99%

Insecticidal activity of endophytic actinomycetes isolated from Azadirachta indica against Myzus persicae

Chen¹,

Shafi²,

Mao-hai³

et al. 2018

Arch biol sci (Beogr)

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“…Viruses, bacteria and fungi can act as biocontrol agents against insects and fungi. The mode of infection of virus and bacteria is via their digestive tract while fungi make entry into the host through the cuticle (Deshpande, 1999 (Gopalakrishnan et al, 2011;Vijayabharathi et al, 2014). This proves the efficiency of microbes as treasure box for biological pesticides.…”

Section: Biological Pesticides From Treasure Boxmentioning

confidence: 91%

Microbial agents against Helicoverpa armigera: Where are we and where do we need to go?

Vijayabharathi¹,

Kumari²,

Gopalakrishnan³

2014

Afr. J. Biotechnol.

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Plants are prone to various biotic stresses in nature by bacteria, viruses, fungi, parasites, harmful insects and weeds. The biggest percentage loss (70%) in plants is attributed to insects. Lepidoptera is one such diversified phytophagous insect group, which include Helicoverpa armigera, a key pest of many food crops including chickpea, pigeonpea, pea, lentil, chillies, sunflower, tomato, tobacco and cotton. Controlling this insect has been a big task for farmers leading to the manufacture of a plethora of pesticides. However, over reliance on chemical pesticides has resulted in problems including safety risks, environmental contamination, outbreaks of secondary pests, insecticide resistance and decrease in biodiversity. Hence, there is an urgent need for the development of eco-friendly methods such as entomopathogens, antagonist or competitor populations of a third organism and botanicals to suppress H. armigera. Also, many compounds from microorganisms have been found to be effective in crop production, and these have a role in controlling H. armigera. The actinomycetes play an astounding role in controlling the key plant pathogens. They are the representative genera of higher microbial mass in the soil. Numerous studies have shown that these productive actino-bacteria can generate an impressive array of secondary metabolites such as antibiotics, antitumor agents, insecticides etc. This review emphasizes the mechanism behind resistance to insecticides along with actinomycetes and its potential as a biocontrol agent against H. armigera.

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“…The most potential antagonistic isolates against B. cinerea were identified by 16 S rDNA analysis at Macrogen Inc. Seoul, Korea [27]. The sequences were submitted to GenBank, NCBI and accession numbers were obtained.…”

Section: Molecular Identificationmentioning

confidence: 99%

Deciphering the tri-dimensional effect of endophytic Streptomyces sp. on chickpea for plant growth promotion, helper effect with Mesorhizobium ciceri and host-plant resistance induction against Botrytis cinerea

Vijayabharathi

Gopalakrishnan

Sathya

et al. 2018

Microbial Pathogenesis

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A total of 219 endophytic actinobacteria, isolated from roots, stems and leaves of chickpea, were characterized for antagonistic potential against Botrytis cinerea, causal organism of Botrytis grey mold (BGM) disease, in chickpea. Among them, three most potential endophytes, AUR2, AUR4 and ARR4 were further characterized for their plant growth-promoting (PGP) and nodulating potentials and host-plant resistance against B. cinerea, in chickpea. The sequences of 16 S rDNA gene of the three endophytes were matched with Streptomyces but different species. In planta, the isolate AUR4 alone was able to significantly enhance PGP traits including seed numbers (11.8 vs. 9.8/Plant), seed weight (8 vs. 6.8 g/Plant), pod numbers (13.6 vs. 11.5/Plant), pod weight (9.3 vs. 7.5 g/Plant) and biomass (10.9 vs. 8 g/Plant) over the un-inoculated control in chickpea genotype JG11. Interestingly, consortium of the selected endophytes, AUR2, AUR4 and ARR4 were found less effective than single inoculation. Co-inoculation of the selected endophytes with Mesorhizobium ciceri significantly enhanced nodulation and nitrogenase activity in five chickpea genotypes including ICCV2, ICCV10, ICC4958, Annigeri and JG11 over the un-inoculated control. The selected endophytes showed antagonistic potential in planta by significant reduction of disease incidence (28─52%) in both single inoculation and consortium treatments over the un-inoculated control across the genotypes ICC4954 (susceptible), ICCV05530 (moderately resistant) and JG11 (unknown resistance). Further, antioxidant enzymes such as superoxide dismutase, catalase, ascorbate peroxidase, guaiacol peroxidase, glutathione reductase, phenylalanine ammonia-lyase and polyphenol oxidase and phenolics were found induced in the leaves of chickpea inoculated with selected endophytes over un-inoculated control. Principal component analysis revealed that, the antioxidant enzymes and phenolics were found in the magnitude of ICC4954 < JG11 < ICCV05530 which correlates with their resistance level. The selected endophytes enhanced the plant growth and also host plant resistance against BGM in chickpea.

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Biological activity of entomopathogenic actinomycetes against lepidopteran insects (Noctuidae: Lepidoptera)

Cited by 35 publications

References 39 publications

Insecticidal activity of endophytic actinomycetes isolated from Azadirachta indica against Myzus persicae

Insecticidal activity of endophytic actinomycetes isolated from Azadirachta indica against Myzus persicae

Microbial agents against Helicoverpa armigera: Where are we and where do we need to go?

Deciphering the tri-dimensional effect of endophytic Streptomyces sp. on chickpea for plant growth promotion, helper effect with Mesorhizobium ciceri and host-plant resistance induction against Botrytis cinerea

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