Biological Activity of Endophytic Fungi from the Roots of the Medicinal Plant Vernonia anthelmintica

Rustamova, Nigora; Gao, Yanhua; Zhang, Yong; Yili, Abulimiti

doi:10.3390/microorganisms8040586

Cited by 24 publications

(24 citation statements)

References 52 publications

(72 reference statements)

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“…and Schizophyllum sp., with the remaining two belonging to Aspergillus sp. isolated from the fresh root of the medicinal plant Vernonia anthelmintica, were examined for biological activities by Rustamova and colleagues [67]. The ethyl acetate extracts of these fungi expressed antimicrobial, cytotoxic, and antioxidant, antidiabetic, melanin content, and tyrosinase activity on murine B16 cells.…”

Section: Fungimentioning

confidence: 99%

“…The ethyl acetate extracts of these fungi expressed antimicrobial, cytotoxic, and antioxidant, antidiabetic, melanin content, and tyrosinase activity on murine B16 cells. The ethyl acetate extracts from these fungi have been suggested to contain compounds such as steroids, terpenes, or terpenoids [67]. The antibacterial activities of 24ξhydroperoxy-24-vinyllathosterol which is a steroid isolated from V. anthelmintica, were reported to be antibacterial against B. cereus, S. aureus, B. subtilis, and E. coli, with minimum inhibition concentrations ranging from 3.15 to 15.5 µg/mL [68].…”

Section: Fungimentioning

confidence: 99%

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Production and Functionalities of Specialized Metabolites from Different Organic Sources

et al. 2022

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Medicinal plants are rich sources of specialized metabolites that are of great importance to plants, animals, and humans. The usefulness of active biological compounds cuts across different fields, such as agriculture, forestry, food processing and packaging, biofuels, biocatalysts, and environmental remediation. In recent years, research has shifted toward the use of microbes, especially endophytes (bacteria, fungi, and viruses), and the combination of these organisms with other alternatives to optimize the production and regulation of these compounds. This review reinforces the production of specialized metabolites, especially by plants and microorganisms, and the effectiveness of microorganisms in increasing the production/concentration of these compounds in plants. The study also highlights the functions of these compounds in plants and their applications in various fields. New research areas that should be explored to produce and regulate these compounds, especially in plants and microbes, have been identified. Methods involving molecular studies are yet to be fully explored, and next-generation sequencing possesses an interesting and reliable approach.

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Section: Fungimentioning

confidence: 99%

Section: Fungimentioning

confidence: 99%

Production and Functionalities of Specialized Metabolites from Different Organic Sources

et al. 2022

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“…The rhizosphere soil of the sampled roots was removed by physical treatment, including hand shaking, hairbrush cleaning, and thoroughly water washing. Then, successively, all tissues were rinsed in sterile distilled water for six times, 70% ethanol for 2 min, 5.25% sodium hypochlorite for 4 min, and sterile distilled water for five times at last (Rustamova et al, 2020). In order to confirm the complete sterilization of the surface, 100 µl of the final eluate is reserved for plate culture as the control.…”

Section: Endophytes Isolationmentioning

confidence: 99%

Root Endophytes and Ginkgo biloba Are Likely to Share and Compensate Secondary Metabolic Processes, and Potentially Exchange Genetic Information by LTR-RTs

Zou

Liu

Hu³

et al. 2021

Front. Plant Sci.

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Ginkgo biloba is a pharmaceutical resource for terpenes and flavonoids. However, few insights discussed endophytes’ role in Ginkgo, and whether genetic exchange happens between Ginkgo and endophytes remains unclear. Herein, functional gene profiles and repetitive sequences were analyzed to focus on these issues. A total of 25 endophyte strains were isolated from the Ginkgo root and distributed in 16 genera of 6 phyla. Significant morphological diversities lead to the diversity in the COG functional classification. KEGG mapping revealed that endophytic bacteria and fungi potentially synthesize chalcone, while endophytic fungi might also promote flavonoid derivatization. Both bacteria and fungi may facilitate the lignin synthesis. Aspergillus sp. Gbtc_1 exhibited the feasibility of regulating alcohols to lignans. Although Ginkgo and the endophytes have not observed the critical levopimaradiene synthase in ginkgolides synthesis, the upstream pathways of terpenoid precursors are likely intact. The MVK genes in Ginkgo may have alternative non-homologous copies or be compensated by endophytes in long-term symbiosis. Cellulomonas sp. Gbtc_1 became the only bacteria to harbor both MEP and MVA pathways. Endophytes may perform the mutual transformation of IPP and DMAPP in the root. Ginkgo and bacteria may lead to the synthesis and derivatization of the carotenoid pathway. The isoquinoline alkaloid biosynthesis seemed lost in the Ginkgo root community, but L-dopa is more probably converted into dopamine as an essential signal-transduction substance. So, endophytes may participate in the secondary metabolism of the Ginkgo in a shared or complementary manner. Moreover, a few endophytic sequences predicted as Ty3/Gypsy and Ty1/Copia superfamilies exhibited extremely high similarity to those of Ginkgo. CDSs in such endophytic LTR-RT sequences were also highly homologous to one Ginkgo CDS. Therefore, LTR-RTs may be a rare unit flowing between the Ginkgo host and endophytes to exchange genetic information. Collectively, this research effectively expanded the insight on the symbiotic relationship between the Ginkgo host and the endophytes in the root.

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“…[8] Metabolites from endophytes support growth of the host, aid with improvement to tolerate biotic/abiotic stress, increase resistance to pest/ insect attack or organic toxins. [9] The products of such interactions, the metabolites exhibit broad spectrum pharmaceutical property. These metabolites are pilots of pharmaceutical revolution leading to discoveries of drug with a range of biological activities are a boon.…”

Section: Isolation Of Genomic Dna Of Endophytes Its Amplification By ...mentioning

confidence: 99%

“…Secondary metabolites conferring biological activity comprise phenols, alkaloids, quinones, steroids, polyketides, terpenoids, and lignans. [9] These exhibit assorted biological properties such as antioxidant, antibacterial, antidiabetic, cytotoxicity, antimalarial, anti-tubercular and acetylcholinesterase hindrance. [10][11][12] The endophytes are todays` established expedient supply of natural ad-mixture with therapeutic values.…”

Section: Isolation Of Genomic Dna Of Endophytes Its Amplification By ...mentioning

confidence: 99%

Buchanania lanzan fungal endophyte Penicillium sp. therapeutic properties attributed to lapachol in its extract

Sharon

Shenoy

Bhaskar

et al. 2021

Int. J. Pharm. Sci. Drug Res.

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Buchanania lanzan, Spr. (Family: Anacardiaceae) with known therapeutic potential is reported as vulnerable and is listed in Red Data Book of IUCN. With immense value for its bark, roots, seeds and gum, exploitation of the host on large scale is reported detrimental. Hence the authors have attempted to study the residing endophytes for metabolites equivalent to the host products. The leaf endophytic fungi were morphotyped based on internal transcribed spacer–deoxyribonucleic acid (ITS–DNA) sequences and B. lanzan endophytes identified by molecular typing include Penicillium sp, Fusarium sp., Aspergillus sp and Fusarium sp. Molecular typing identified Penicillium sp. as Penicillium gravinicasei with 98% similarity to the nearest genera and so its extract was screened for therapeutic capacities. Antioxidant activity of the extract exhibited 2,2-diphenyl-1-picrylhydrazyl (DPPH) scavenging capacity with IC50 of 55.95 ± 3.29 μg.mL-1. Antimicrobial activity by disc diffusion in opposition to Pseudomonas aeruginosa, Staphylococcus aureus, Escherichia coli and Bacillus subtilis significantly arrested bacterial growth. Bacterial biofilm inhibition capacity stained by acridine orange and ethidium bromide imaged by confocal laser scanning microscopy revealed bactericidal activity. Lapachol in the endophyte extract detected by TLC could support the therapeutic properties. Thus, studies on unexplored medicinal plant endophytes could pave path to identification of novel secondary metabolites as therapeutic agents and potential drug candidates.

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Biological Activity of Endophytic Fungi from the Roots of the Medicinal Plant Vernonia anthelmintica

Cited by 24 publications

References 52 publications

Production and Functionalities of Specialized Metabolites from Different Organic Sources

Production and Functionalities of Specialized Metabolites from Different Organic Sources

Root Endophytes and Ginkgo biloba Are Likely to Share and Compensate Secondary Metabolic Processes, and Potentially Exchange Genetic Information by LTR-RTs

Buchanania lanzan fungal endophyte Penicillium sp. therapeutic properties attributed to lapachol in its extract

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