Biological activity of carbonic nano-structures—comparison via enzymatic bioassay

Sachkova, Anna S.; Kovel, Ekaterina S.; Чурилов, Г. Н.; Стом, Д. И.; Kudryasheva, Nadezhda S.

doi:10.1007/s11368-018-2134-9

Cited by 23 publications

(31 citation statements)

References 40 publications

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“…In contrast to Fe 3 O 4 /HA, water solutions of HA did not show any low-concentration inhibitory effects under the conditions of the oxidative stress, but opposite, revealed an antioxidant activity and mitigation of the oxidative effect of Bq (i.e., T rel Bq > 1) [49,52,58]. Hence, humic acids-coated MNPs, Fe 3 O 4 /HA, show higher oxidative toxicity than its components Fe 3 O 4 or HA under the conditions of the oxidative stress.…”

Section: The Relative Bioluminescence Intensity I Relmentioning

confidence: 85%

“…Changes in general toxicity and oxidative toxicity of aqueous media under exposure to humic substances were studied previously in [47][48][49]. Later [41,[50][51][52], the toxicity and antioxidant activity of a series of fullerenols (i.e., carbon nanostructures, water-soluble derivatives of fullerenes, perspective pharmaceutical agents) were evaluated and compared. Effects of MNPs and MNPs with modified surface on bacterial enzyme reactions have not been studied yet.…”

Section: Introductionmentioning

confidence: 99%

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Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions

et al. 2020

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Current paper presents biological effects of magnetite nanoparticles (MNPs). “Relations of MNP’ characteristics (zeta-potential and hydrodynamic diameters) with effects on bacteria and their enzymatic reactions were the main focus.”. Photobacterium phosphoreum and bacterial enzymatic reactions were chosen as bioassays. Three types of MNPs were under study: bare Fe3O4, Fe3O4 modified with 3-aminopropyltriethoxysilane (Fe3O4/APTES), and humic acids (Fe3O4/HA). Effects of the MNPs were studied at a low concentration range (< 2 mg/L) and attributed to availability and oxidative activity of Fe3+, high negative surface charge, and low hydrodynamic diameter of Fe3O4/HA, as well as higher Fe3+ content in suspensions of Fe3O4/HA. Low-concentration suspensions of bare Fe3O4 provided inhibitory effects in both bacterial and enzymatic bioassays, whereas the MNPs with modified surface (Fe3O4/APTES and Fe3O4/HA) did not affect the enzymatic activity. Under oxidative stress (i.e., in the solutions of model oxidizer, 1,4-benzoquinone), MNPs did not reveal antioxidant activity, moreover, Fe3O4/HA demonstrated additional inhibitory activity. The study contributes to the deeper understanding of a role of humic substances and silica in biogeochemical cycling of iron. Bioluminescence assays, cellular and enzymatic, can serve as convenient tools to evaluate bioavailability of Fe3+ in natural dispersions of iron-containing nanoparticles, e.g., magnetite, ferrihydrite, etc.

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Section: The Relative Bioluminescence Intensity I Relmentioning

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions

et al. 2020

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“…Figure 11. Antioxidant activity of humic substances was studied in [80][81][82][83][84] and fullerenols -in [84][85][86][87]. Cellular and enzymatic assays were used to evaluate a General Toxicity (GT) under conditions of the oxidative stress; this stress was modeled in the solutions of exogenous organic or inorganic oxidizers (1,4-benzoquinone or potassium ferricianide, respectively).…”

Section: Luminescence Bioassays As Tools For Study Low-dose Radiationmentioning

confidence: 99%

“…The latter appears in the presence of Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 21 August 2019 doi:10.20944/preprints201908.0221.v1exogenous oxidizers, depending on their redox potential and concentration[25]. Justification for GTand OxT application to evaluate toxicity of bioactive compounds is presented in[80][81][82][83][84][85][86][87].Principle of antioxidant efficiency evaluation is presented inFigure forbacteria-based (upper line) and enzyme-based (lower line) bioassays; humic substances are chosen here as an example of an antioxidant agent.…”

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confidence: 99%

Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

Kudryasheva¹,

Kovel²

2019

Preprint

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Current paper reviews applications of luminescence bioassays for monitoring low-intensity factors, namely, radioactivity of different types (alpha, beta and gamma), and bioactive compounds (humic substances and fullerenols). Luminescence intensity is taken as a physiological parameter of luminous organisms. High rates of luminescence response can provide (1) a proper number of experiments under comparable conditions and, therefore, proper statistical processing, with this being highly important for ‘noisy’ low-dose exposures; (2) non-genetic, i.e. biochemical and physicochemical mechanisms of cellular response, in accordance to “exposome” concept. Bioassays based on luminous marine bacteria, their enzymes, and fluorescence coelenteramide-containing proteins were used to compare results of low-intensity exposures at cellular, biochemical and physicochemical levels, respectively. Results of the cellular exposures were discussed in terms of hormesis concept. Bioluminescence time dependence under low-dose radiation exposures corresponded to hormesis or threshold models; no bioluminescence monotonic dependency on intensity of exposure (dose rate, radioactivity, concentration) was found. Bioluminescence activation and absence of its dependency on intensity of exposure can be accepted as features of cellular adaptive response. Changes of biological luminescence were analyzed and discussed for bioassays of lower organization level – enzymes and florescent protein.

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“…Decrease of both general and oxidative toxicities under addition of humic substances (natural bioactive compounds, products of decomposition of organic matter in soils and bottom sediments) was studied in [51][52][53]. Description of the enzyme bioluminescence technique to evaluate antioxidant activity and toxicity of bioactive compounds is presented in [54][55][56].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Toxicity of Fullerenols with Different Number of Hydroxyl Substituents

Kovel¹,

Sachkova²,

Vnukova³

et al. 2018

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Fullerenols are nanosized water-soluble polyhydroxylated derivatives of fullerenes, specific allotropic form of carbon, bioactive compounds and perspective pharmaceutical agents. We studied biological effects of a series of fullerenols. Antioxidant activity and toxicity of the fullerenols were compared using bioluminescence assays (cellular and enzymatic); a content of Reactive Oxygen Species in fullerenol solutions was determined using chemiluminescence luminol method. Two groups of fullerenols with different number of hydroxyl substituents were under investigation: (I) С60Оy(OH)x, С60,70Оy(OH)x, where х+у=24–28 and (II) С60,70Оy(OH)x, Fe0,5С60Оy(OH)x, where х+у=40–42. Toxicity of the fullerenols was evaluated using effective concentrations ЕС50. Fullerenol’ antioxidant activity was investigated in model solutions of organic toxicant of oxidative type, 1,4-benzoquinone. Detoxification coefficients were calculated to analyze and compare the antioxidant activity. Higher toxicity and lower antioxidant activity were demonstrated in the solutions of fullerenols with higher number of the oxygen substituents (х+у=40–42). The differences were concerned with fullerenol’ ability to disturb Reactive Oxygen Species balance in aqueous solutions. Toxic effect of the prospective endohedral metal-fullerenol with gadolinium atom involved, Gd@C82Oy(OH)x, where х+у=40–42, was evaluated and explained by a high number of oxygen groups

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Biological activity of carbonic nano-structures—comparison via enzymatic bioassay

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Cited by 23 publications

References 40 publications

Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions

Effects of Modified Magnetite Nanoparticles on Bacterial Cells and Enzyme Reactions

Low-intensity Exposures via Luminescent Bioassays of Different Complexity: Cells, Enzyme Reactions and Fluorescent Proteins

Antioxidant Activity and Toxicity of Fullerenols with Different Number of Hydroxyl Substituents

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