Biological activity of Brassica rapa L. polysaccharides on RAW264.7 macrophages and on tumor cells

Wufuer, Reziyamu; Bai, Jie; Li, Zhan; Zhou, Kai; Taoerdahong, Hailiqian

doi:10.1016/j.bmc.2020.115330

Cited by 25 publications

(4 citation statements)

References 27 publications

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“…The above results indicated that CCP2 holded a strong potential to stimulate macrophages, which was the key participant in innate and adaptive immunity. Compared with previous reports, CCP2 showed stronger effect on the activation of the phagocytosis of macrophages ( 22 , 34 , 39 ).…”

Section: Resultscontrasting

confidence: 85%

Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides

Zhang

Shi

et al. 2022

Front. Nutr.

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In this study, we investigated the structural features of the polysaccharide obtained from Craterellus cornucopioides (CCP2) by high-performance liquid chromatography, Fourier transform infrared spectroscopy and ion chromatography. The results showed that CCP2 was a catenarian pyranose that principally comprised of mannose, galactose, glucose, and xylose in the ratio of 1.86: 1.57: 1.00: 1.14, with a molecular weight of 8.28 × 104 Da. Moreover, the immunoregulation effect of CCP2 was evaluated both in vitro and in vivo. It displayed a remarkable immunological activity and activation in RAW264.7 cells by enhancing the phagocytosis of macrophages in a dose-dependent manner without showing cytotoxicity at the concentrations of 10–200 μg/mL in vitro. Additionally, Histopathological analysis indicated the protective function of CCP2 against immunosuppression induced by cyclophosphamide (Cy). Meanwhile, the intake of CCP2 had better immunoregulatory activity for immunosuppression BALB/c mice model. After prevention by CCP2, the spleen and thymus weight indexes of BALB/c mice model were significantly increased. The RT-qPCR and Western Blot results provided comprehensive evidence that the CCP2 could activate macrophages by enhancing the production of cytokines (IL-2, IL-6, and IL-8) and upregulating the protein expression of cell membrane receptor TLR4 and its downstream protein kinase (TRAF6, TRIF, and NF-κB p65) production of immunosuppressive mice through TLR4-NFκB p65 pathway. The results demonstrated that CCP2 could be a potential prebiotic and might provide meaningful information for further research on the immune mechanism.

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Section: Resultscontrasting

confidence: 85%

Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides

Zhang

Shi

et al. 2022

Front. Nutr.

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“…26 On isolated macrophages, ethanolic and ethyl acetate extracts of B. rapa seeds both inhibited macrophage proliferation. In contrast, other authors observed stimulation of macrophages under Brassica roots extract 12,28 or by aqueous extract of total plant from Brassica campestris with the experiments conducted on macrophage cell line RAW 264.7 and not on isolated macrophages 11 We indicated, for the first time, an inhibition of freshly isolated macrophage proliferation under B. rapa seeds extracts leading to an antiinflammatory effect of compounds present in B. rapa extracts. This conclusion is confirmed by previous publications indicating an antiinflammatory effect of B. rapa root compounds on cultured macrophage cell lines.…”

Section: Discussioncontrasting

confidence: 69%

“…Most research so far was carried out on other parts (leaves, roots… etc) and only a few scientific studies on the effects of the seeds of these plants on immunity were carried out. 28,30,31 In Moroccan culture, seeds of both plants (Brassica and Raphanus) are indicated by traditional herbalists to treat infections but also included in formulas to treat cancers. In this study, our goal was to verify the traditional herbalist use of these seeds in formulas by exploring their immunostimulant impact.…”

Section: Introductionmentioning

confidence: 99%

Immunostimulant effect of Brassica rapa and Raphanus sativus seeds on thymic cells and their cytotoxicity

Laaraj

Ouahidi

Aarab

2023

EJI

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Immunomodulators from natural products are widely used for the treatment of infectious diseases, allergies, and cancer in traditional Moroccan medicine. The purpose of this study was the study of two plant seeds of Brassica rapa (Turnip) and Raphanus sativus (Radish) used by Moroccans in traditional medicine to enhance immunity. We have prepared three different extracts from seeds using ethanol, Ethyl Acetate, or water. Immunomodulatory effects of these two plant seeds were tested on rabbit immunity cell proliferation (splenocytes, thymocytes, and macrophages) and their functions (IgG production, cytotoxicity, and phagocytosis). The results obtained indicated that only aqueous extract of B. rapa seeds revealed an immunostimulant effect on both splenocyte and thymocyte proliferation with an increase in cytotoxicity of thymocytes (MLR assay). With R. sativus seeds, we observed an important stimulation of thymocyte proliferation and their cytotoxicity under aqueous extract without effect on splenocyte or macrophages. We concluded that aqueous extract of both seeds (B. rapa and R. sativus) possessed immunostimulant properties leading to stimulation of cellular immunity responsible for defense against viruses.

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“…To further investigate the immunological activity of AMP-ZnONPs on RAW264.7 cells, the cytokine (IL-1β and IL-6) contents in cells were evaluated by RT-qPCR in this study ( Figures 6C,D ). LPS stimulated the production of IL-1β and IL-6 by more than 2,000 times compared to the control group ( P < 0.001), which indicated that LPS could promote inflammation and lead to excessive cytokine release ( 52 ). Notably, AMP-ZnONPs treatment exerted a significant action on IL-1β and IL-6 secretion than both AMP and ZnONPs ( P < 0.001), which showed ZnONPs exerted a synergistic effect with AMP.…”

Section: Resultsmentioning

confidence: 99%

Polysaccharide from Atractylodes macrocephala Koidz binding with zinc oxide nanoparticles: Characterization, immunological effect and mechanism

Liu

Wang

et al. 2022

Front. Nutr.

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Atractylodes macrocephala Koidz (A. macrocephala) has been used both as a traditional medicine and functional food for hundreds of years in Asia. And it has a variety of biological activities, such as enhancing the ability of immunity and modulating effect on gastrointestinal motility. In this study, a watersoluble polysaccharide with molecular weight of 2.743 × 10 3 Da was isolated from the root of A. macrocephala. Polysaccharide from A. macrocephala (AMP) consisted of arabinose, galactose, glucose, xylose, mannose, ribose, galactose uronic acid, glucose uronic acid, with a percentage ratio of 21.86, 12.28, 34.19, 0.43, 0.92, 0.85, 28.79, and 0.67%, respectively. Zinc plays an important role in immune system. Therefore, we supposed that AMP binding with zinc oxide (ZnO) nanoparticles (AMP-ZnONPs) might be an effective immunostimulator. AMP-ZnONPs was prepared by Borch reduction, and its structural features were characterized by Scanning Electron Microscope (SEM), Transmission electron microscope (TEM), TEM-energy dispersive spectroscopy mapping (TEM-EDS mapping), Fourier transform infrared spectroscopy (FT-IR), X-ray photoelectron spectrometer (XPS), X-ray diffraction (XRD), particle size and zeta-potential distribution analysis. Then, its immunostimulatory activity and the underlying mechanism were evaluated using RAW264.7 cells. The results showed that AMP-ZnONPs remarkably promoted cell proliferation, enhanced phagocytosis, the release of nitric oxide (NO), cytokines (IL-6 and IL-1β) and the expression of co-stimulatory molecules (CD80, CD86 and MHCII). Moreover, AMP-ZnONPs could promote Frontiers in Nutrition 01 frontiersin.org Bo et al. 10.3389/fnut.2022.992502the expression of Toll-like receptor 4 (TLR4), Myeloid differentiation factor 88 (MyD88), TNF receptor associated factor 6 (TRAF6), phospho-IκBα (P-IκBα) and phospho-p65 (P-p65), and TLR4 inhibitor (TAK242) inhibited the expression of these proteins induced by AMP-ZnONPs. Therefore, AMP-ZnONPs activated macrophages by TLR4/MyD88/NF-κB signaling pathway, indicating that AMP-ZnONPs could act as a potential immunostimulator in medicine and functional food. KEYWORDSpolysaccharide from Atractylodes macrocephala Koidz, zinc oxide nanoparticles, immunostimulatory activity, TLR4 signaling pathways, potential immunostimulator Materials and methods Reagents and materialsA. macrocephala was purchased from the Tongrentang Company in Beijing. The plant material was identified by Prof. Jingui Li. The purified AMP was prepared in our laboratory and the polysaccharide content was 96% (UV). RAW264.7 cells were obtained from American Type Culture Collection (ATCC,

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Biological activity of Brassica rapa L. polysaccharides on RAW264.7 macrophages and on tumor cells

Cited by 25 publications

References 27 publications

Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides

Extraction and immunomodulatory activity of the polysaccharide obtained from Craterellus cornucopioides

Immunostimulant effect of Brassica rapa and Raphanus sativus seeds on thymic cells and their cytotoxicity

Polysaccharide from Atractylodes macrocephala Koidz binding with zinc oxide nanoparticles: Characterization, immunological effect and mechanism

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