Biological Activity of Bacillus thuringiensis in
            Drosophila suzukii (Diptera: Drosophilidae)

Cossentine, J. E.; Robertson, M. C.; Xu, De-Xiang

doi:10.1093/jee/tow062

Cited by 35 publications

(37 citation statements)

References 43 publications

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“…suzukii was the most pronounced, and its horizontal transfer between sexes and its ability to achieve mortality rates exceeding 95% even at 20°C makes this strain a promising candidate as part of a control strategy. However, these studies confirm that the success of a biocontrol is likely to depend on the species or even strain of the biocontrol agent, as previously reported (Cossentine, Robertson, & Buitenhuis, ; Cossentine, Robertson, & Xu, ; Woltz et al., ).…”

Section: Natural Enemies and Pathogens As Control Measuressupporting

confidence: 89%

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Environmentally sustainable pest control options forDrosophila suzukii

Schetelig

Lee

Otto

et al. 2017

J Applied Entomology

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The spotted-wing drosophila or cherry vinegar fly (Drosophila suzukii) is native to Asia but has invaded other continents since 2008 and has spread throughout Europe. The females have a serrated ovipositor allowing them to penetrate the skins of intact ripening fruits to deposit their eggs, and the developing larvae rapidly destroy the fruits close to harvest. Drosophila suzukii has a rapid life cycle and the larvae develop well beneath the fruit surface. This means that the use of pesticides is problematic and often not effective, first due to their restricted use close to harvest to protect consumers, and second because the larvae are deep enough inside the fruit to avoid contact.There are currently no effective and environmentally sustainable pest control methods for this species, resulting in extensive damage to fruit crops. Here, we review the current status of D. suzukii as a fruit crop pest and discuss the feasibility of current pesticide-free control methods. We also consider the potential of new technologies as a basis for the urgently needed specific and long-term control of this species. K E Y W O R D Scherry vinegar fly, integrated pest management, invasive insects, RNA interference, sterile insect technique

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Section: Natural Enemies and Pathogens As Control Measuressupporting

confidence: 89%

“…fumosorosea or Lecanicillium lecanii conidia may lead to the infection of adult D . suzukii and result in dose‐dependent mortality (Cossentine, Robertson, & Buitenhuis, ; Cossentine, Robertson, & Xu, ). The virulence of M .…”

Section: Natural Enemies and Pathogens As Control Measuresmentioning

confidence: 99%

Environmentally sustainable pest control options forDrosophila suzukii

Schetelig

Lee

Otto

et al. 2017

J Applied Entomology

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“…israeliensis (Solbac) is active against larvae of Culicidae and Sciaridae. Cossentine, Robertson, and Xu () found lethal effects of one B. t . serovars var.…”

Section: Discussionmentioning

confidence: 99%

Screening of 25 different natural crop protection products against Drosophila suzukii

Cahenzli

Strack

Daniel

2018

J Applied Entomology

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Drosophila suzukii is a major pest of soft‐skinned fruits, and insecticides are often used to prevent fruit damage caused by oviposition. As D. suzukii produces many generations per year, repeated insecticide applications are required. Furthermore, D. suzukii attacks ripening and ripe fruits shortly before harvest. Therefore, the use of synthetic insecticides is limited by long pre‐harvest intervals and maximum residue limits. To be able to offer producers immediate and sustainable solutions, we tested 25 natural crop protection products with three different application methods in a laboratory screening. We show that application method is an important factor for the efficacy of the tested products. Of six natural insecticides, only Spinosad was toxic for D. suzukii and reduced the oviposition on treated blueberries. The tested oil products had no control effect and products based on different entomopathogenic fungi and Bacillus thuringiensis rather enhanced oviposition. Mineral products (Kaolin, CaCO3, Ca(OH)2 and clinoptilolith) applied as spray solutions were not toxic, but significantly reduced oviposition on blueberries. We provide the first study in which different application methods have been used to compare numerous, commercially available, natural crop protection products with different modes of action against adult D. suzukii. Our findings provide consultants and producers with important insights for the development of sustainable pest control strategies against D. suzukii.

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“…This invasive species is a pest of stone fruits and berries, causing direct economic loss estimated for different countries to range from few millions up to several hundred million US dollars (Bolda et al, 2010;Asplen et al, 2015;De Ros et al, 2015;Benito et al, 2016). Several pest-control tools against D. suzukii are developed including the use of insecticides and biopesticides (Cossentine et al, 2016;Murphy et al, 2016;Renkema et al, 2016;Smirle et al, 2016). However, currently there is no effective method available for a sustainable management of this pest.…”

Section: Introductionmentioning

confidence: 99%

CRISPR/Cas‐mediated gene editing using purified protein in Drosophila suzukii

Kalajdzic

Schetelig

2017

Entomologia Exp Applicata

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The spotted wing drosophila, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae), is an invasive pest of stone fruits and berries currently without effective control management. The sterile insect technique (SIT) is an environmentally friendly and effective pest control method that releases sterile males to mate with wild females resulting in the suppression or eradication of targeted pest populations. New molecular technologies and genome editing methods are paving the way to bring this technology to invasive species like D. suzukii. The CRISPR/Cas technology is emerging as a versatile option for efficient tailored gene manipulation. Components of this system, the guide RNA (gRNA) and Cas9 protein, can be delivered into the organism in the form of DNA, RNA, or protein. We report CRISPR/Cas-mediated site-specific white gene editing using purified Cas9 protein delivered directly into D. suzukii embryos. Mutant flies with 13, 3, and 1 nt deletions within the cutting site of the Cas9 protein were retrieved. Moreover, the two independent G 1 deletion events (13 and 3 nt) derived from the same G 0 female. Our results show that the recombinant Cas9 protein could be a method of choice for generating heritable gene modifications in D. suzukii.

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Biological Activity of Bacillus thuringiensis in Drosophila suzukii (Diptera: Drosophilidae)

Cited by 35 publications

References 43 publications

Environmentally sustainable pest control options forDrosophila suzukii

Environmentally sustainable pest control options forDrosophila suzukii

Screening of 25 different natural crop protection products against Drosophila suzukii

CRISPR/Cas‐mediated gene editing using purified protein in Drosophila suzukii

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